FastQCFastQC Report
Fri 10 Feb 2017
ERR1633300.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1633300.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences923886
Sequences flagged as poor quality0
Sequence length43
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT39600.4286243107915912No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT27430.2968981021468016No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT15850.17155796277895757No Hit
CTCCAGGACAGGCTGCATCAGAAGAGGCCATCAAGCAGATCAC15100.16344007810487443No Hit
GTATTAGAGGCACCGCCTGCCCAGTGACACATGTTTAACGGCC13250.14341596257546926No Hit
TTATTACCCTCACTGTCAACCCAACACAGGCATGCTCATAAGG10770.11657282391983426No Hit
GTACATGCTAAGACTTCACCAGTCAAAGCGAACTACTATACTC10340.11191857004002657No Hit
GTATGAATGGCTCCACGAGGGTTCAGCTGTCTCTTACTTTTAA9840.10650664692397115No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA9300.024.4677411
GTATCAA24400.023.2008191
TCGCCGT1301.8189894E-1221.34615325
CGAGTCG1303.274181E-1119.92307721
CCGCTCT1950.019.92307728
TTAACGG2900.019.77586235
GTATTAG4350.019.5632171
GCCGGCA1900.019.47368415
TGTCCGG2000.019.42514
TTCGCCG1950.018.9743624
GCCGCTC2150.018.93023327
TTTAACG3200.018.534
CCGTCCG1501.2732926E-1118.528
GCACCGC3000.018.510
TTGGTCG609.2349475E-418.510
CGCCGTC1501.2732926E-1118.526
AGCTAGT701.2188686E-418.56
GAGGCAC3350.018.2238797
AGCTTCG1950.018.02564221
AACGGCC3200.017.92187537
Produced by FastQC (version 0.11.5)