##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	ERR1633300.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	923886
Sequences flagged as poor quality	0
Sequence length	43
%GC	46
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.813091658494663	33.0	33.0	33.0	27.0	33.0
2	31.941657304039676	33.0	33.0	33.0	33.0	33.0
3	31.65969069777007	33.0	33.0	33.0	27.0	33.0
4	31.951095697954077	33.0	33.0	33.0	33.0	33.0
5	32.048419393734726	33.0	33.0	33.0	33.0	33.0
6	35.21167113691516	37.0	37.0	37.0	33.0	37.0
7	35.35338234370907	37.0	37.0	37.0	33.0	37.0
8	35.49008210969752	37.0	37.0	37.0	33.0	37.0
9	35.606578084309106	37.0	37.0	37.0	33.0	37.0
10	35.61520901929459	37.0	37.0	37.0	33.0	37.0
11	35.6348088400517	37.0	37.0	37.0	33.0	37.0
12	35.61855683493418	37.0	37.0	37.0	33.0	37.0
13	35.62645824268362	37.0	37.0	37.0	33.0	37.0
14	35.59528881268901	37.0	37.0	37.0	33.0	37.0
15	35.63025524794185	37.0	37.0	37.0	33.0	37.0
16	35.61637799468765	37.0	37.0	37.0	33.0	37.0
17	35.5914550063536	37.0	37.0	37.0	33.0	37.0
18	35.57903788995612	37.0	37.0	37.0	33.0	37.0
19	35.565105435086146	37.0	37.0	37.0	33.0	37.0
20	35.56601463816965	37.0	37.0	37.0	33.0	37.0
21	35.56892192326759	37.0	37.0	37.0	33.0	37.0
22	35.46976033839673	37.0	37.0	37.0	33.0	37.0
23	35.52002195076016	37.0	37.0	37.0	33.0	37.0
24	35.51946019314071	37.0	37.0	37.0	33.0	37.0
25	35.53587996787483	37.0	37.0	37.0	33.0	37.0
26	35.42856477963731	37.0	37.0	37.0	33.0	37.0
27	35.43677358461975	37.0	37.0	37.0	33.0	37.0
28	35.45862476539313	37.0	37.0	37.0	33.0	37.0
29	35.461143474411344	37.0	37.0	37.0	33.0	37.0
30	35.47238295633877	37.0	37.0	37.0	33.0	37.0
31	35.457614900539674	37.0	37.0	37.0	33.0	37.0
32	35.44803146708577	37.0	37.0	37.0	33.0	37.0
33	35.44443686775208	37.0	37.0	37.0	33.0	37.0
34	35.42931270741195	37.0	37.0	37.0	33.0	37.0
35	35.36896759989869	37.0	37.0	37.0	33.0	37.0
36	35.4022401032162	37.0	37.0	37.0	33.0	37.0
37	35.39595686047846	37.0	37.0	37.0	33.0	37.0
38	35.38136523337295	37.0	37.0	37.0	33.0	37.0
39	35.28285849119913	37.0	37.0	37.0	33.0	37.0
40	35.0867823519352	37.0	37.0	37.0	33.0	37.0
41	35.248788270414316	37.0	37.0	37.0	33.0	37.0
42	35.29287054896383	37.0	37.0	37.0	33.0	37.0
43	34.95563954860231	37.0	37.0	37.0	27.0	37.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
14	6.0
15	27.0
16	42.0
17	38.0
18	22.0
19	28.0
20	68.0
21	134.0
22	491.0
23	1008.0
24	2126.0
25	3781.0
26	6194.0
27	9230.0
28	13201.0
29	17504.0
30	22673.0
31	29197.0
32	37005.0
33	49060.0
34	70884.0
35	145106.0
36	516061.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	41.76694960200717	19.913171105526008	13.626356498529038	24.69352279393778
2	16.408626172493143	21.164407729958025	38.81810093453089	23.60886516301795
3	17.961956345263378	27.193181842781467	29.645757160515473	25.19910465143968
4	12.544513067629556	17.685948266344546	39.43603431592209	30.3335043501038
5	14.141355102252875	36.9024966283719	34.839688013456204	14.11646025591902
6	29.755186245922115	39.42098916965946	17.16597069335394	13.65785389106448
7	27.191233550459685	31.814531230043535	23.06063735136153	17.933597868135244
8	24.964660142052157	35.321240932322816	20.230418038589175	19.483680887035845
9	26.570918922897413	15.246902756400683	19.85299052047547	38.329187800226435
10	15.830957499085386	27.504042706567695	32.98188304617669	23.683116748170228
11	34.31386556350026	23.605509770685995	23.634950632437334	18.445674033376413
12	23.90262434975744	25.44426476859699	29.303507142656127	21.349603738989444
13	28.25316110429209	22.045685290176493	25.60391650052063	24.09723710501079
14	22.519769755142953	20.65969178015469	27.485317452586145	29.335221012116214
15	24.560497723745137	27.84564329365311	24.563203685303165	23.030655297298587
16	23.20383683701236	27.793147639427374	24.792885702348556	24.210129821211705
17	22.83701668820612	26.891196532905575	25.8606581331463	24.411128645742007
18	23.70682097141855	26.462896937500947	27.252280043208792	22.578002047871706
19	25.658252208605823	25.451192030185542	26.863595725013695	22.026960036194943
20	24.943012449587936	25.650026085469417	26.936440210155798	22.470521254786846
21	23.959016588626735	26.36548232141195	27.055069564859735	22.62043152510158
22	23.495323016043105	26.382800475383327	26.79886912454567	23.3230073840279
23	23.26726457593253	26.394490229314005	27.463453283197275	22.874791911556187
24	23.693615879015375	26.699181500747926	26.1103642657211	23.4968383545156
25	23.48612274674581	26.245229389773193	27.373398882546113	22.895248980934877
26	23.73777717164239	26.50229573778583	27.1297541038613	22.63017298671048
27	23.781938464269402	26.383991098468858	26.297075613225008	23.536994824036732
28	22.97047471224805	26.02095929584386	27.623105015120913	23.38546097678718
29	24.558008239111754	25.873105556313224	26.529247114903786	23.039639089671237
30	24.02763977373832	25.831541986781918	27.170451765694036	22.970366473785727
31	23.6060509629976	25.962402287728136	27.227276958412617	23.20426979086164
32	22.58406340176169	25.813682640498936	27.487590460294886	24.114663497444493
33	22.659180894612536	26.12335288119963	27.76089257765568	23.456573646532146
34	23.572280562753413	25.988487757147528	27.3897428903566	23.049488789742455
35	23.694048832864663	26.464195799048802	26.68998123145063	23.151774136635904
36	24.331681614398313	26.11880686578214	26.41245781405931	23.137053705760234
37	23.568383978109853	25.821584048248376	26.843138655635002	23.76689331800677
38	23.91766949602007	26.183425227787843	26.537797953427155	23.36110732276493
39	23.02113031261433	26.23884332049625	27.13678960391217	23.60323676297725
40	23.455383023446615	26.523185761013806	27.313975966731824	22.707455248807754
41	23.28685573761265	25.508883130602694	27.38259915184341	23.821661979941247
42	22.738411449031588	26.829500609382546	26.888707048272188	23.543380893313678
43	23.775444156530135	25.485720099665976	27.05755904949312	23.68127669431077
>>END_MODULE
>>Per sequence GC content	pass
#GC Content	Count
0	27.0
1	42.5
2	58.0
3	199.5
4	341.0
5	341.0
6	427.0
7	513.0
8	492.5
9	472.0
10	670.0
11	868.0
12	868.0
13	1372.0
14	1876.0
15	3393.0
16	4910.0
17	5543.0
18	6176.0
19	6176.0
20	5491.5
21	4807.0
22	5105.0
23	5403.0
24	7353.0
25	9303.0
26	9303.0
27	12304.5
28	15306.0
29	18409.0
30	21512.0
31	26296.0
32	31080.0
33	31080.0
34	38781.0
35	46482.0
36	51235.0
37	55988.0
38	60232.0
39	64476.0
40	64476.0
41	67835.5
42	71195.0
43	72843.5
44	74492.0
45	73609.5
46	72727.0
47	72727.0
48	70205.0
49	67683.0
50	68732.0
51	69781.0
52	66940.0
53	64099.0
54	64099.0
55	62498.0
56	60897.0
57	53240.0
58	45583.0
59	41686.0
60	37789.0
61	37789.0
62	33726.0
63	29663.0
64	24049.0
65	18435.0
66	15596.0
67	12757.0
68	12757.0
69	10900.5
70	9044.0
71	7675.5
72	6307.0
73	6163.0
74	6019.0
75	6019.0
76	5103.0
77	4187.0
78	3121.5
79	2056.0
80	1364.0
81	672.0
82	672.0
83	533.5
84	395.0
85	337.0
86	279.0
87	216.0
88	153.0
89	153.0
90	105.0
91	57.0
92	37.0
93	17.0
94	9.0
95	1.0
96	1.0
97	0.5
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
43	923886.0
>>END_MODULE
>>Sequence Duplication Levels	warn
#Total Deduplicated Percentage	52.51165440009831
#Duplication Level	Percentage of deduplicated	Percentage of total
1	85.52107250934975	44.90853003536722
2	8.22545900195948	8.638649207861476
3	2.117834927086954	3.336330473029426
4	1.029668490520477	2.1627838368352874
5	0.5768600333664113	1.5145937354683086
6	0.36577930164102795	1.1524605764689784
7	0.27482265441893183	1.0101974575115218
8	0.21143210924418868	0.8882119879771735
9	0.16007212391140052	0.7565086844932659
>10	1.2110518700089306	12.661751558573675
>50	0.16855443889170732	6.17475355658414
>100	0.12840033381816388	12.664505685620108
>500	0.007528358329654958	2.677458878270798
>1k	0.0014638474529884641	1.4532643259386326
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT	3960	0.4286243107915912	No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT	2743	0.2968981021468016	No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT	1585	0.17155796277895757	No Hit
CTCCAGGACAGGCTGCATCAGAAGAGGCCATCAAGCAGATCAC	1510	0.16344007810487443	No Hit
GTATTAGAGGCACCGCCTGCCCAGTGACACATGTTTAACGGCC	1325	0.14341596257546926	No Hit
TTATTACCCTCACTGTCAACCCAACACAGGCATGCTCATAAGG	1077	0.11657282391983426	No Hit
GTACATGCTAAGACTTCACCAGTCAAAGCGAACTACTATACTC	1034	0.11191857004002657	No Hit
GTATGAATGGCTCCACGAGGGTTCAGCTGTCTCTTACTTTTAA	984	0.10650664692397115	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	5.411923116055444E-4	0.0	0.0
2	2.1647692464221775E-4	0.0	5.411923116055444E-4	0.0	0.0
3	2.1647692464221775E-4	0.0	6.494307739266533E-4	0.0	0.0
4	2.1647692464221775E-4	0.0	9.741461608899799E-4	3.2471538696332667E-4	0.0
5	2.1647692464221775E-4	0.0	9.741461608899799E-4	3.2471538696332667E-4	0.0
6	2.1647692464221775E-4	0.0	0.002056530784101069	3.2471538696332667E-4	0.0
7	2.1647692464221775E-4	0.0	0.004978969266771009	3.2471538696332667E-4	0.0
8	2.1647692464221775E-4	0.0	0.00584487696533988	3.2471538696332667E-4	0.0
9	2.1647692464221775E-4	0.0	0.007684930824798731	0.0011906230855321977	0.0
10	4.329538492844355E-4	0.0	0.00876731544800982	0.0012988615478533067	0.0
11	4.329538492844355E-4	0.0	0.01168975393067976	0.0016235769348166332	0.0
12	5.411923116055444E-4	0.0	0.013205092403175283	0.001731815397137742	0.0
13	5.411923116055444E-4	0.0	0.014287477026386372	0.001731815397137742	0.0
14	5.411923116055444E-4	0.0	0.014720430875670808	0.001731815397137742	0.0
15	5.411923116055444E-4	0.0	0.015586338574239678	0.001840053859458851	0.0
16	5.411923116055444E-4	0.0	0.01645224627280855	0.002056530784101069	0.0
17	5.411923116055444E-4	0.0	0.016885200122092985	0.002056530784101069	0.0
18	6.494307739266533E-4	0.0	0.017209915509056314	0.0022730077087432863	0.0
19	7.576692362477621E-4	0.0	0.01742639243369853	0.0022730077087432863	0.0
20	7.576692362477621E-4	0.0	0.017642869358340748	0.0023812461710643953	0.0
21	7.576692362477621E-4	0.0	0.0182923001322674	0.0030306769449910485	0.0
22	7.576692362477621E-4	0.0	0.01861701551923073	0.003571869256596593	0.0
23	7.576692362477621E-4	0.0	0.019049969368515163	0.0038965846435599196	0.0
24	7.576692362477621E-4	0.0	0.019049969368515163	0.004546015417486573	0.0
25	7.576692362477621E-4	0.0	0.01915820783083627	0.005087207729092117	0.0
26	7.576692362477621E-4	0.0	0.01926644629315738	0.005303684653734335	0.0
27	7.576692362477621E-4	0.0	0.01937468475547849	0.006386069276945424	0.0
28	7.576692362477621E-4	0.0	0.019482923217799597	0.01645224627280855	0.0
29	7.576692362477621E-4	0.0	0.019482923217799597	0.034636307942754845	0.0
30	7.576692362477621E-4	0.0	0.019482923217799597	0.060288823512857644	0.0
31	7.576692362477621E-4	0.0	0.019482923217799597	0.12274241627213747	0.0
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GGTATCA	930	0.0	24.467741	1
GTATCAA	2440	0.0	23.200819	1
TCGCCGT	130	1.8189894E-12	21.346153	25
CGAGTCG	130	3.274181E-11	19.923077	21
CCGCTCT	195	0.0	19.923077	28
TTAACGG	290	0.0	19.775862	35
GTATTAG	435	0.0	19.563217	1
GCCGGCA	190	0.0	19.473684	15
TGTCCGG	200	0.0	19.425	14
TTCGCCG	195	0.0	18.97436	24
GCCGCTC	215	0.0	18.930233	27
TTTAACG	320	0.0	18.5	34
CCGTCCG	150	1.2732926E-11	18.5	28
GCACCGC	300	0.0	18.5	10
TTGGTCG	60	9.2349475E-4	18.5	10
CGCCGTC	150	1.2732926E-11	18.5	26
AGCTAGT	70	1.2188686E-4	18.5	6
GAGGCAC	335	0.0	18.223879	7
AGCTTCG	195	0.0	18.025642	21
AACGGCC	320	0.0	17.921875	37
>>END_MODULE