FastQCFastQC Report
Fri 10 Feb 2017
ERR1633299.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1633299.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences487715
Sequences flagged as poor quality0
Sequence length43
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT125642.576094645438422No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT102472.10102211332438No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT58661.2027516069835866No Hit
ATCATTAACTGAATCCATAGGTTAATGAGGCGAACCGGGGGAA23710.48614457213741635No Hit
GTATGAAACTGCTCGCCGGCAGCTTCGCCGCTCTCTTCCTGAG22870.46892139876772293No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT21620.44329167649139356No Hit
GTTAATGATAGTGTGTCGAAACACACTGGGTTTCCCCATTCGG19340.39654306305936865No Hit
GTACTGGTTCACTATCGGTCAGTCAGGAGTATTTAGCCTTGGA19020.3899818541566284No Hit
TCTTTAGTGACCATGAAGTGCGATTGCCTCGGTGATAACGACA17100.35061460074018636No Hit
ACCATGAAGTGCGATTGCCTCGGTGATAACGACATTGGGGCCA15970.3274453318023846No Hit
CCGTATTACCGCGGCTGCTGGCACGGAGTTAGCCGGTGCTTCT15810.3241647273510144No Hit
GTATGAAACTGCTTGTCCGGCGAGTCGCCGTCCGCCGCCTGGG15650.32088412289964424No Hit
ATACAGGGTGACAGCCCCGTACACAAAAATGCACATGCTGTGA15090.3094020073198487No Hit
ATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTT14870.3048911761992147No Hit
GTTAATACCTTTGCTCATTGACGTTACCCGCAGAAGAAGCACC13440.2755707739150938No Hit
TCTTTAGTGGCCCCAATGTCGTTATCACCGAGGCAATCGCACT12590.25814256276718983No Hit
TATTCAGACAGGATACCACGTGTCCCGCCCTACTCATCGAGCT11580.23743374716791568No Hit
TCATTAACCTATGGATTCAGTTAATGATAGTGTGTCGAAACAC11490.23558840716401996No Hit
CATCTTTAGTGACCATGAAGTGCGATTGCCTCGGTGATAACGA11290.23148765159980728No Hit
GGATACCACGTGTCCCGCCCTACTCATCGAGCTCACAGCATGT10140.2079083071055842No Hit
GAGTTAGCCGGTGCTTCTTCTGCGGGTAACGTCAATGAGCAAA10070.20647304265810976No Hit
GTGGATGCCCTGGCAGTCAGAGGCGATGAAGGACGTGCTAATC9920.20339747598495023No Hit
CCCATATTCAGACAGGATACCACGTGTCCCGCCCTACTCATCG9910.2031924382067396No Hit
GTCATCTTTAGTGGCCCCAATGTCGTTATCACCGAGGCAATCG9640.19765641819505242No Hit
GGTATTAACTTTACTCCCTTCCTCCCCGCTGAAAGTACTTTAC9500.19478588930010354No Hit
AGCGTACACGGTGGATGCCCTGGCAGTCAGAGGCGATGAAGGA9400.19273551151799717No Hit
CCCCAATGTCGTTATCACCGAGGCAATCGCACTTCATGGTCAC9350.191710322626944No Hit
GGTTAATGAGGCGAACCGGGGGAACTGAAACATCTAAGTACCC9230.1892498692884164No Hit
CCGTCATCTTTAGTGACCATGAAGTGCGATTGCCTCGGTGATA9100.1865843781716781No Hit
GTATCAACGCAGAGTACGGGGATTTCACATCTGACTTAACAAA9010.18473903816778242No Hit
GTAATACGGAGGGTGCAAGCGTTAATCGGAATTACTGGGCGTA8910.18268866038567605No Hit
GGTGATATGAACCGTTATAACCGGCGATTTCCGAATGGGGAAA8890.1822785848292548No Hit
TAATGATAGTGTGTCGAAACACACTGGGTTTCCCCATTCGGAA8710.17858790482146333No Hit
CCTATGGATTCAGTTAATGATAGTGTGTCGAAACACACTGGGT8480.17387203592261874No Hit
TATCAACGCAGAGTACGGGGATTTCACATCTGACTTAACAAAC8470.1736669981444081No Hit
CTATCGGTCAGTCAGGAGTATTTAGCCTTGGAGGATGGTCCCC8350.1712065448058805No Hit
TCACGGTACTGGTTCACTATCGGTCAGTCAGGAGTATTTAGCC8240.1689511292455635No Hit
GTATTTAGCCTTGGAGGATGGTCCCCCCATATTCAGACAGGAT8190.1679259403545103No Hit
ATGATAGTGTGTCGAAACACACTGGGTTTCCCCATTCGGAAAT8090.16587556257240396No Hit
CGTTATCACCGAGGCAATCGCACTTCATGGTCACTAAAGATGA8090.16587556257240396No Hit
CCGTCATCTTTAGTGGCCCCAATGTCGTTATCACCGAGGCAAT8020.16444029812492952No Hit
CATCTTTAGTGGCCCCAATGTCGTTATCACCGAGGCAATCGCA7870.16136473145177No Hit
GATATGAACCGTTATAACCGGCGATTTCCGAATGGGGAAACCC7780.15951939144787428No Hit
ACCCTGTATCGCGCGCCTTTCCAGACGCTTCCACTAACACACA7680.15746901366576793No Hit
GTCAGGAGTATTTAGCCTTGGAGGATGGTCCCCCCATATTCAG7550.15480352254902965No Hit
GTTATAACCGGCGATTTCCGAATGGGGAAACCCAGTGTGTTTC7470.15316322032334456No Hit
GTCGTTATCACCGAGGCAATCGCACTTCATGGTCACTAAAGAT7290.14947254031555315No Hit
GTGGTATCCTGTCTGAATATGGGGGGACCATCCTCCAAGGCTA7240.14844735142449997No Hit
CGGTACTGGTTCACTATCGGTCAGTCAGGAGTATTTAGCCTTG7030.14414155808207663No Hit
GTGCTAATCTGCGATAAGCGTCGGTAAGGTGATATGAACCGTT6900.14147606696533838No Hit
GAATATGGGGGGACCATCCTCCAAGGCTAAATACTCCTGACTG6840.14024584029607456No Hit
GTATTACCGCGGCTGCTGGCACGGAGTTAGCCGGTGCTTCTTC6750.13840050029217882No Hit
GTATCAACGCAGAGTACGGGACTAGTATGAAACTGCTTGTCCG6720.1377853869575469No Hit
GGCTAACTCCGTGCCAGCAGCCGCGGTAATACGGAGGGTGCAA6690.137170273622915No Hit
GTCATCTTTAGTGACCATGAAGTGCGATTGCCTCGGTGATAAC6670.13676019806649375No Hit
CGCTTGCACCCTCCGTATTACCGCGGCTGCTGGCACGGAGTTA6620.13573500917544057No Hit
CTTTACGCCCAGTAATTCCGATTAACGCTTGCACCCTCCGTAT6570.1347098202843874No Hit
GTTCACTATCGGTCAGTCAGGAGTATTTAGCCTTGGAGGATGG6500.13327455583691294No Hit
GCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT6280.12876372471627898No Hit
ACTTAGATGTTTCAGTTCCCCCGGTTCGCCTCATTAACCTATG6240.12794357360343644No Hit
GTGTGTGTGTTAGTGGAAGCGTCTGGAAAGGCGCGCGATACAG6120.12548312026490882No Hit
GTCTGAATATGGGGGGACCATCCTCCAAGGCTAAATACTCCTG6110.12527808248669817No Hit
CTCCGTATTACCGCGGCTGCTGGCACGGAGTTAGCCGGTGCTT6090.12486800693027691No Hit
GTATCAACGCAGAGTACGGGCTCCGTCATCTTTAGTGACCATG6060.12425289359564501No Hit
ATCTTTAGTGGCCCCAATGTCGTTATCACCGAGGCAATCGCAC6010.12322770470459184No Hit
GGAGTAAAGTTAATACCTTTGCTCATTGACGTTACCCGCAGAA5960.12220251581353864No Hit
GTATGAAGAAGGCCTTCGGGTTGTAAAGTACTTTCAGCGGGGA5810.11912694914037912No Hit
AGTTAATACCTTTGCTCATTGACGTTACCCGCAGAAGAAGCAC5780.11851183580574721No Hit
GTAAAGTACTTTCAGCGGGGAGGAAGGGAGTAAAGTTAATACC5760.11810176024932593No Hit
CGTGGTATCCTGTCTGAATATGGGGGGACCATCCTCCAAGGCT5750.11789672247111531No Hit
CCGTTATAACCGGCGATTTCCGAATGGGGAAACCCAGTGTGTT5730.11748664691469404No Hit
GCCTGAATCAGTGTGTGTGTTAGTGGAAGCGTCTGGAAAGGCG5610.11502619357616639No Hit
CAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT5590.11461611801974513No Hit
CACTATCATTAACTGAATCCATAGGTTAATGAGGCGAACCGGG5570.11420604246332386No Hit
TTAGTGGCCCCAATGTCGTTATCACCGAGGCAATCGCACTTCA5570.11420604246332386No Hit
CTATCATTAACTGAATCCATAGGTTAATGAGGCGAACCGGGGG5470.11215566468121752No Hit
TGTATGAAGAAGGCCTTCGGGTTGTAAAGTACTTTCAGCGGGG5460.11195062690300688No Hit
TCGGTAAGGTGATATGAACCGTTATAACCGGCGATTTCCGAAT5440.11154055134658561No Hit
GGGTTTCCCCATTCGGAAATCGCCGGTTATAACGGTTCATATC5430.11133551356837497No Hit
ATCCATAGGTTAATGAGGCGAACCGGGGGAACTGAAACATCTA5410.1109254380119537No Hit
GAGTACGGGGATTTCACATCTGACTTAACAAACCGCCTGCGTG5400.11072040023374306No Hit
CATCTGACTTAACAAACCGCCTGCGTGCGCTTTACGCCCAGTA5390.11051536245553244No Hit
ATATTGCACAATGGGCGCAAGCCTGATGCAGCCATGCCGCGTG5380.1103103246773218No Hit
GGTTCACTATCGGTCAGTCAGGAGTATTTAGCCTTGGAGGATG5360.10990024912090053No Hit
CGGTAATACGGAGGGTGCAAGCGTTAATCGGAATTACTGGGCG5230.10723475800416228No Hit
GTCCAGACTCCTACGGGAGGCAGCAGTGGGGAATATTGCACAA5220.10702972022595163No Hit
TCTAAGTACCCCGAGGAAAAGAAATCAACCGAGATTCCCCCAG5220.10702972022595163No Hit
AATACGGAGGGTGCAAGCGTTAATCGGAATTACTGGGCGTAAA5120.10497934244384527No Hit
CAGTAATTCCGATTAACGCTTGCACCCTCCGTATTACCGCGGC5090.10436422910921336No Hit
GCCTTGGAGGATGGTCCCCCCATATTCAGACAGGATACCACGT5050.10354407799637083No Hit
CATTAACCTATGGATTCAGTTAATGATAGTGTGTCGAAACACA5030.10313400243994957No Hit
GGGTGACAGCCCCGTACACAAAAATGCACATGCTGTGAGCTCG4950.10149370021426449No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CCCAGAT200.001840897637.07
TAACAAC352.3855102E-531.7142851
TCGCAGG250.0054932829.634
TGCCCAG250.0054932829.65
GAGAGGT250.0054932829.69
TACGCCG250.0054932829.631
CTAGTGC250.0054932829.69
GGTCTAA250.0054932829.61
AGAGGAG250.0054932829.65
TAAGAGG250.0054932829.63
AGATGGG250.0054932829.610
GTGCCCA250.0054932829.64
ACAACGC454.0020386E-628.7777793
GATGTGC405.9332277E-527.751
AGCAACG556.249338E-726.909092
TAGCAAC556.249338E-726.909091
CCTAGTT358.8620454E-426.428572
GAATCAA358.8620454E-426.428572
TTGTAGG358.8620454E-426.428573
GGTATCA24700.024.7914981
Produced by FastQC (version 0.11.5)