FastQCFastQC Report
Fri 10 Feb 2017
ERR1633298.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1633298.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences608167
Sequences flagged as poor quality0
Sequence length43
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT26640.43803757849406494No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT19500.32063561488867365No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT11640.1913947978104698No Hit
GATATGGACAATACTAGTTAAGAAAGCTAACAGGATGTGAAAG8260.13581795789643306No Hit
GTATTAGAGGCACCGCCTGCCCAGTGACACATGTTTAACGGCC8210.13499581529415441No Hit
GTACATGCTAAGACTTCACCAGTCAAAGCGAACTACTATACTC7940.1305562452418497No Hit
ATCATTAACTGAATCCATAGGTTAATGAGGCGAACCGGGGGAA7700.12660996075091216No Hit
ATACTAGTTAAGAAAGCTAACAGGATGTGAAAGTCTTCCAGAA7370.12118381957587307No Hit
GTATGAATGGCTCCACGAGGGTTCAGCTGTCTCTTACTTTTAA7140.11740196360539128No Hit
AGCATGTACTGCTCGGAGGTTGGGTTCTGCTCCGAGGTCGCCC7060.11608653544174545No Hit
TTATTACCCTCACTGTCAACCCAACACAGGCATGCTCATAAGG6710.11033153722579489No Hit
TGCTAAGACTTCACCAGTCAAAGCGAACTACTATACTCAATTG6140.10095911155981827No Hit
GATAGAAACCGACCTGGATTACTCCGGTCTGAACTCAGATCAC6110.10046582599845108No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA5800.026.1551721
CGTTATT451.3222115E-424.66666610
GTATCAA15600.022.1762831
CCGGCAG957.1286195E-921.42105316
AGCTTCG1059.804353E-1021.14285721
GCTTCGC1059.804353E-1021.14285722
CGCCGTT555.139268E-420.18181825
GTATAAC656.895303E-519.9230773
CGGGAAT759.254012E-619.73333417
CTATTAG851.2433356E-619.5882361
TCGGTAG851.2433356E-619.58823628
TTCGCCG1052.2540917E-819.3809524
ACCGCAT500.00703116518.58
GCCGGCA1103.842797E-818.515
TTATACC801.6146307E-518.54
ACTAGTG701.2179996E-418.58
CGCTCTC1205.158654E-918.529
TTGCGGT701.2179996E-418.535
ACGTCGC500.00703116518.516
TAGGACC1156.39011E-817.6956524
Produced by FastQC (version 0.11.5)