FastQCFastQC Report
Fri 10 Feb 2017
ERR1633297.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1633297.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1113702
Sequences flagged as poor quality0
Sequence length43
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT66760.5994422206299351No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT47820.42937877457344964No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT25050.22492551867555233No Hit
GCTTTATTGAGCCTGTGTGGGAGCAGGGAGCAAGCTTTGGCCA19910.17877313679961068No Hit
ATCATTAACTGAATCCATAGGTTAATGAGGCGAACCGGGGGAA11930.10712021707781794No Hit
GTATTAGAGGCACCGCCTGCCCAGTGACACATGTTTAACGGCC11690.10496524204859109No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT11660.10469587016993774No Hit
GTACTGGTTCACTATCGGTCAGTCAGGAGTATTTAGCCTTGGA11550.1037081732815421No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTTACG454.0081104E-628.77777716
GTATCAA36450.023.1947881
GGTATCA13900.021.9604321
TTTACCG1001.2896635E-820.3530
CGCTCTC3600.020.04166629
CCGTTTA1254.129106E-1019.2427
ACGGTAC1551.8189894E-1219.0967733
GCCGGCA3500.019.02857215
GCTTTAT4300.018.9302331
CCGCTCT3650.018.75342428
TTCGCCG3950.018.73417724
GCCGCTC3700.018.527
GTTACGG1409.458745E-1118.517
TTACCGG1103.8513463E-818.531
GGACCGT1750.017.971436
TAGGACC3000.017.8833334
GTCGCCC3000.017.26666637
ATCGTTA752.0671937E-417.26666614
CGTGTTT650.001579892717.07692316
GCTTCGC4350.017.01149422
Produced by FastQC (version 0.11.5)