FastQCFastQC Report
Fri 10 Feb 2017
ERR1633296.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1633296.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences644016
Sequences flagged as poor quality0
Sequence length43
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT51630.8016881568159797No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT39700.6164443119425604No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT22260.34564358649474547No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT8610.1336923306253261No Hit
ATCATTAACTGAATCCATAGGTTAATGAGGCGAACCGGGGGAA7300.11335122108767486No Hit
GTATGAAACTGCTCGCCGGCAGCTTCGCCGCTCTCTTCCTGAG6920.10745074656530273No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTAGCG250.00549457129.615
GCGGAAT250.00549457129.619
CGTCAGG250.00549457129.61
GGTATCA10600.025.655661
GTATCAA26250.021.7771421
TTATACT1201.0913936E-1121.5833344
CGGGAAT705.0961753E-621.14285917
CCGGTCT705.0961753E-621.14285924
GTATTGG555.139719E-420.1818181
GCTCGGA1101.7498678E-920.18181811
TTCGCCG1600.019.6562524
GTCGCCA851.2435685E-619.58823612
TCGCCAT801.614872E-518.513
ACGGGTT609.2308037E-418.515
TATTGTC1501.2732926E-1118.55
CGCTCTC1800.018.529
GCTTCGC1800.018.522
CGGTCTG801.614872E-518.525
TCCGGTC801.614872E-518.523
GTACTGC1351.1477823E-917.8148166
Produced by FastQC (version 0.11.5)