##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	ERR1633296.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	644016
Sequences flagged as poor quality	0
Sequence length	43
%GC	45
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	30.93512894089588	33.0	33.0	33.0	27.0	33.0
2	31.313021726168294	33.0	33.0	33.0	27.0	33.0
3	31.05405455765074	33.0	33.0	33.0	27.0	33.0
4	31.378451777595586	33.0	33.0	33.0	27.0	33.0
5	31.51528533452585	33.0	33.0	33.0	27.0	33.0
6	34.07436616481578	37.0	33.0	37.0	27.0	37.0
7	34.28254888077315	37.0	33.0	37.0	27.0	37.0
8	34.41482354475665	37.0	37.0	37.0	27.0	37.0
9	34.50422505030931	37.0	37.0	37.0	27.0	37.0
10	34.47931417852973	37.0	37.0	37.0	27.0	37.0
11	34.58570594519391	37.0	37.0	37.0	27.0	37.0
12	34.594831494869695	37.0	37.0	37.0	27.0	37.0
13	34.59663890338128	37.0	37.0	37.0	27.0	37.0
14	34.63644847332986	37.0	37.0	37.0	27.0	37.0
15	34.63366282825272	37.0	37.0	37.0	27.0	37.0
16	34.62735087327023	37.0	37.0	37.0	27.0	37.0
17	34.63462087898437	37.0	37.0	37.0	27.0	37.0
18	34.58973379543365	37.0	37.0	37.0	27.0	37.0
19	34.57568290228814	37.0	37.0	37.0	27.0	37.0
20	34.55537284787955	37.0	37.0	37.0	27.0	37.0
21	34.57357736453753	37.0	37.0	37.0	27.0	37.0
22	34.460418685250055	37.0	37.0	37.0	27.0	37.0
23	34.489995590171674	37.0	37.0	37.0	27.0	37.0
24	34.51106183697299	37.0	37.0	37.0	27.0	37.0
25	34.505721907530244	37.0	37.0	37.0	27.0	37.0
26	34.37662728876301	37.0	37.0	37.0	27.0	37.0
27	34.38917356090532	37.0	37.0	37.0	27.0	37.0
28	34.397083302278205	37.0	37.0	37.0	27.0	37.0
29	34.38648573948473	37.0	37.0	37.0	27.0	37.0
30	34.39641872251621	37.0	37.0	37.0	27.0	37.0
31	34.38438330724703	37.0	37.0	37.0	27.0	37.0
32	34.352821979578145	37.0	37.0	37.0	27.0	37.0
33	34.32325904946461	37.0	37.0	37.0	27.0	37.0
34	34.30869264117662	37.0	37.0	37.0	27.0	37.0
35	34.236955293036196	37.0	37.0	37.0	27.0	37.0
36	34.256425306203575	37.0	37.0	37.0	27.0	37.0
37	34.25343314451815	37.0	37.0	37.0	27.0	37.0
38	34.21977249012447	37.0	37.0	37.0	27.0	37.0
39	34.09761558719038	37.0	37.0	37.0	27.0	37.0
40	33.85973329855159	37.0	33.0	37.0	27.0	37.0
41	34.05539613922635	37.0	33.0	37.0	27.0	37.0
42	34.07092991478473	37.0	37.0	37.0	27.0	37.0
43	33.5743987727013	37.0	33.0	37.0	27.0	37.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
14	1.0
15	23.0
16	49.0
17	35.0
18	44.0
19	77.0
20	187.0
21	498.0
22	1088.0
23	2178.0
24	3800.0
25	6202.0
26	9132.0
27	12571.0
28	16448.0
29	21356.0
30	26753.0
31	32594.0
32	40623.0
33	53011.0
34	75560.0
35	131410.0
36	210376.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	41.64508335196641	20.755850786315868	14.295762838190356	23.303303023527366
2	16.144940498372712	23.683573078929715	37.920641723187	22.250844699510573
3	18.347059700380115	28.203336563066756	30.83805371295123	22.611550023601897
4	13.0535576755857	19.448895679610445	38.793756676852745	28.703789967951103
5	14.42883406623438	37.13867357332737	34.52305532781794	13.909437032620309
6	27.87881046433629	41.00690044967827	17.261372388263645	13.852916697721795
7	25.547657201063327	33.39295918113835	23.036539464858016	18.022844152940298
8	24.738515813271718	35.219156045812525	20.613928846488285	19.428399294427468
9	26.753062035725822	15.209094183995427	20.103071973367122	37.93477180691163
10	15.176175747186404	28.39991553004894	33.351500583836426	23.072408138928225
11	33.1983987975454	24.36973615562346	23.82518446746665	18.606680579364486
12	22.88110854388711	26.755701721696358	30.14645598867109	20.216733745745447
13	28.93763508981143	22.750055899232315	26.32217211994733	21.99013689100892
14	22.33950709299148	21.846972746018732	28.845246080842713	26.968274080147076
15	24.040085960597253	28.69866587165536	25.467224416784678	21.794023750962708
16	21.84138282278701	29.053004894288343	25.80541477230379	23.300197510620855
17	22.08392338078557	27.676641574122385	26.61082954460759	23.628605500484458
18	23.48342277210504	26.225745944200145	28.342152989987827	21.948678293706987
19	25.061489155548934	25.95277135971777	28.840277260192295	20.145462224541006
20	24.898605003602395	25.588184144493304	28.45379617897692	21.05941467292738
21	22.873189485975505	26.98690715758615	28.217466646791383	21.922436709646963
22	22.505962584780505	26.85119624357159	28.352245906933987	22.290595264713918
23	22.185939479764478	26.729304861991004	29.071948523018058	22.012807135226453
24	22.48220541104569	26.850264589699634	28.6224255298005	22.045104469454174
25	22.521800700603713	26.744056048296937	29.127071377108642	21.60707187399071
26	22.575526073886362	26.95895754142754	28.45022483913443	22.015291545551662
27	22.675989416412015	26.64700876996845	28.43376562072992	22.24323619288962
28	22.090444957889243	26.879301135375517	28.685933268738665	22.34432063799657
29	22.793377804278155	26.891412635710914	28.189206479342126	22.126003080668806
30	22.66248043526869	26.88194082134605	28.681585550669546	21.77399319271571
31	22.756888027626644	26.756322824277657	28.624133561899086	21.862655586196617
32	21.68641772875208	26.775887555588678	28.80502968870339	22.732665026955853
33	21.80629052694343	26.73629226603066	29.18483391716976	22.27258328985615
34	22.206591140592778	26.542352985019008	28.69726839084743	22.553787483540784
35	22.10426449032322	27.223081414126355	28.695405083103527	21.977249012446894
36	22.430343345506945	26.88520160989789	28.65627562048148	22.028179424113688
37	22.852693100792525	26.694988944374053	28.29355171300092	22.1587662418325
38	22.785148195075898	26.85756254502994	28.068712578569478	22.288576681324688
39	22.29556408536434	26.684430200491917	28.25240366698964	22.76760204715411
40	22.23997540433778	27.27665151176368	28.482056346426177	22.00131673747236
41	22.34602867009515	26.639400263347497	28.45410673026757	22.560464336289783
42	22.106748900648434	26.951970137387892	28.32833345755385	22.612947504409828
43	22.072122431740826	26.797936697224912	28.465597128021663	22.664343743012598
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	35.0
1	46.5
2	58.0
3	214.5
4	371.0
5	371.0
6	522.0
7	673.0
8	695.5
9	718.0
10	974.0
11	1230.0
12	1230.0
13	1968.0
14	2706.0
15	5067.5
16	7429.0
17	8095.5
18	8762.0
19	8762.0
20	7952.5
21	7143.0
22	7208.0
23	7273.0
24	9030.5
25	10788.0
26	10788.0
27	13114.5
28	15441.0
29	18594.0
30	21747.0
31	24501.5
32	27256.0
33	27256.0
34	30151.5
35	33047.0
36	35157.5
37	37268.0
38	39126.0
39	40984.0
40	40984.0
41	42943.5
42	44903.0
43	44564.5
44	44226.0
45	45344.0
46	46462.0
47	46462.0
48	46130.5
49	45799.0
50	47641.5
51	49484.0
52	48890.5
53	48297.0
54	48297.0
55	43543.5
56	38790.0
57	34586.0
58	30382.0
59	26991.0
60	23600.0
61	23600.0
62	20375.0
63	17150.0
64	13938.5
65	10727.0
66	9217.0
67	7707.0
68	7707.0
69	6350.5
70	4994.0
71	4289.5
72	3585.0
73	2973.5
74	2362.0
75	2362.0
76	1710.5
77	1059.0
78	804.0
79	549.0
80	433.5
81	318.0
82	318.0
83	285.5
84	253.0
85	223.0
86	193.0
87	166.0
88	139.0
89	139.0
90	108.5
91	78.0
92	54.0
93	30.0
94	15.0
95	0.0
96	0.0
97	0.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
43	644016.0
>>END_MODULE
>>Sequence Duplication Levels	warn
#Total Deduplicated Percentage	61.56689321756321
#Duplication Level	Percentage of deduplicated	Percentage of total
1	89.03724432036202	54.81746513457815
2	6.163711889484383	7.589611834474195
3	1.5674318890779606	2.895057352219985
4	0.7455830173850018	1.8361292006468393
5	0.4286972664135696	1.3196779411972746
6	0.29780597310343837	1.1000993127367136
7	0.22887865679482627	0.9863943475866457
8	0.1624566404274601	0.8001560506945199
9	0.144994228035392	0.803415973915619
>10	1.0280898068617013	12.974637362644525
>50	0.12197343509339131	5.075934819218439
>100	0.07032007400086754	7.22661526260212
>500	0.002045674880023216	0.7865318138670239
>1k	5.11418720005804E-4	0.975450584211385
>5k	2.55709360002902E-4	0.812823009406614
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT	5163	0.8016881568159797	No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT	3970	0.6164443119425604	No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT	2226	0.34564358649474547	No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	861	0.1336923306253261	No Hit
ATCATTAACTGAATCCATAGGTTAATGAGGCGAACCGGGGGAA	730	0.11335122108767486	No Hit
GTATGAAACTGCTCGCCGGCAGCTTCGCCGCTCTCTTCCTGAG	692	0.10745074656530273	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	4.6582693597674595E-4	0.0	0.0
2	0.0	0.0	9.316538719534919E-4	0.0	0.0
3	0.0	0.0	9.316538719534919E-4	0.0	0.0
4	0.0	0.0	0.0015527564532558198	0.0	0.0
5	0.0	0.0	0.0023291346798837297	0.0	0.0
6	0.0	0.0	0.004037166778465131	1.5527564532558197E-4	0.0
7	0.0	0.0	0.009005987428883755	1.5527564532558197E-4	0.0
8	0.0	0.0	0.010558743882139574	1.5527564532558197E-4	0.0
9	1.5527564532558197E-4	0.0	0.012422051626046558	6.211025813023279E-4	0.0
10	0.0012422051626046558	0.0	0.014440635015279124	6.211025813023279E-4	0.0
11	0.0013974808079302377	0.0	0.02034110953765124	6.211025813023279E-4	0.0
12	0.0013974808079302377	0.0	0.020962212118953567	6.211025813023279E-4	0.0
13	0.0013974808079302377	0.0	0.023912449380139623	6.211025813023279E-4	0.0
14	0.0013974808079302377	0.0	0.024999378897418698	6.211025813023279E-4	0.0
15	0.0013974808079302377	0.0	0.027173237931976846	6.211025813023279E-4	0.0
16	0.0013974808079302377	0.0	0.028570718739907082	6.211025813023279E-4	0.0
17	0.0013974808079302377	0.0	0.02903654567588383	7.763782266279099E-4	0.0
18	0.0013974808079302377	0.0	0.02981292390251174	9.316538719534919E-4	0.0
19	0.0013974808079302377	0.0	0.030278750838488484	9.316538719534919E-4	0.0
20	0.0013974808079302377	0.0	0.031210404710441977	0.001086929517279074	0.0
21	0.0013974808079302377	0.0	0.032452609873046635	0.0017080320985814017	0.0
22	0.0013974808079302377	0.0	0.0327631611636978	0.0020185833892325655	0.0
23	0.0015527564532558198	0.0	0.0327631611636978	0.0026396859705348935	0.0
24	0.0017080320985814017	0.0	0.03291843680902338	0.0038818911331395495	0.0
25	0.0018633077439069838	0.0	0.03307371245434896	0.0038818911331395495	0.0
26	0.0018633077439069838	0.0	0.03307371245434896	0.004813545005093041	0.0
27	0.0020185833892325655	0.0	0.03307371245434896	0.006055750167697697	0.0
28	0.0020185833892325655	0.0	0.03307371245434896	0.016303942759186108	0.0
29	0.0020185833892325655	0.0	0.03307371245434896	0.04254552681920946	0.0
30	0.0020185833892325655	0.0	0.03307371245434896	0.07608506620953517	0.0
31	0.0020185833892325655	0.0	0.03307371245434896	0.15372288887232616	0.0
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
CGTAGCG	25	0.005494571	29.6	15
GCGGAAT	25	0.005494571	29.6	19
CGTCAGG	25	0.005494571	29.6	1
GGTATCA	1060	0.0	25.65566	1
GTATCAA	2625	0.0	21.777142	1
TTATACT	120	1.0913936E-11	21.583334	4
CGGGAAT	70	5.0961753E-6	21.142859	17
CCGGTCT	70	5.0961753E-6	21.142859	24
GTATTGG	55	5.139719E-4	20.181818	1
GCTCGGA	110	1.7498678E-9	20.181818	11
TTCGCCG	160	0.0	19.65625	24
GTCGCCA	85	1.2435685E-6	19.588236	12
TCGCCAT	80	1.614872E-5	18.5	13
ACGGGTT	60	9.2308037E-4	18.5	15
TATTGTC	150	1.2732926E-11	18.5	5
CGCTCTC	180	0.0	18.5	29
GCTTCGC	180	0.0	18.5	22
CGGTCTG	80	1.614872E-5	18.5	25
TCCGGTC	80	1.614872E-5	18.5	23
GTACTGC	135	1.1477823E-9	17.814816	6
>>END_MODULE