FastQCFastQC Report
Fri 10 Feb 2017
ERR1633295.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1633295.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences803763
Sequences flagged as poor quality0
Sequence length43
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT39260.48845244182675734No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT27720.344877781136977No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT15770.1962021143048386No Hit
GTATTAGAGGCACCGCCTGCCCAGTGACACATGTTTAACGGCC13100.16298336698753238No Hit
TTATTACCCTCACTGTCAACCCAACACAGGCATGCTCATAAGG11270.14021546152286182No Hit
GTACATGCTAAGACTTCACCAGTCAAAGCGAACTACTATACTC10600.1318796709975453No Hit
GTATGAATGGCTCCACGAGGGTTCAGCTGTCTCTTACTTTTAA10450.1300134492381461No Hit
CCCCTATACCTTCTGCATAATGAATTAACTAGAAATAACTTTG10160.12640542050330758No Hit
AGCATGTACTGCTCGGAGGTTGGGTTCTGCTCCGAGGTCGCCC9960.12391712482410859No Hit
CTCCAGGACAGGCTGCATCAGAAGAGGCCATCAAGCAGATCAC9080.11296862383563314No Hit
CTCTAATACTGGTGATGCTAGAGGTGATGTTTTTGGTAAACAG8940.11122681686019387No Hit
TGCTAAGACTTCACCAGTCAAAGCGAACTACTATACTCAATTG8160.1015224637113179No Hit
CCATAGGGTCTTCTCGTCTTGCTGTGTCATGCCCGCCTCTTCA8060.10027831587171841No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ATACCGT250.00549537329.5999986
CGAGTCG951.0913936E-1125.3157921
GTATCAA21700.023.0184331
GGTATCA8050.022.2919251
AGTCGCC1059.822543E-1021.14285923
TTAACGG3700.021.035
TACTAGG450.003824822420.5555552
TCGGCGT555.141246E-420.18181811
CCGAGTT1101.7516868E-920.18181813
TAACGGC3950.020.1392436
AACGGCC3850.019.70129837
GCTCGGA2750.019.50909211
GTCGCCG1153.0468073E-919.30434824
GCCGTCC1153.0468073E-919.30434827
TCGCCGT1254.110916E-1019.2425
GTCCGGC1254.110916E-1019.2415
GTCGCCC2600.019.21153837
TTTAACG4050.019.18518434
CTGCTCG5000.018.8699999
CGGCGTA609.233524E-418.512
Produced by FastQC (version 0.11.5)