##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	ERR1633295.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	803763
Sequences flagged as poor quality	0
Sequence length	43
%GC	46
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.652066591768968	33.0	33.0	33.0	27.0	33.0
2	31.821745962429222	33.0	33.0	33.0	27.0	33.0
3	31.548916782683452	33.0	33.0	33.0	27.0	33.0
4	31.84949543584365	33.0	33.0	33.0	27.0	33.0
5	31.959491790490482	33.0	33.0	33.0	33.0	33.0
6	35.06763685315199	37.0	37.0	37.0	33.0	37.0
7	35.22512979572337	37.0	37.0	37.0	33.0	37.0
8	35.34390983411777	37.0	37.0	37.0	33.0	37.0
9	35.46081369757005	37.0	37.0	37.0	33.0	37.0
10	35.46402359899622	37.0	37.0	37.0	33.0	37.0
11	35.5017498939364	37.0	37.0	37.0	33.0	37.0
12	35.48325439215291	37.0	37.0	37.0	33.0	37.0
13	35.47999223651748	37.0	37.0	37.0	33.0	37.0
14	35.46542575361145	37.0	37.0	37.0	33.0	37.0
15	35.47781746609386	37.0	37.0	37.0	33.0	37.0
16	35.46328084273598	37.0	37.0	37.0	33.0	37.0
17	35.443160483873974	37.0	37.0	37.0	33.0	37.0
18	35.42629979235173	37.0	37.0	37.0	33.0	37.0
19	35.41809090490605	37.0	37.0	37.0	33.0	37.0
20	35.4158178468031	37.0	37.0	37.0	33.0	37.0
21	35.42527088208838	37.0	37.0	37.0	33.0	37.0
22	35.32915175244444	37.0	37.0	37.0	33.0	37.0
23	35.37544276111242	37.0	37.0	37.0	33.0	37.0
24	35.359512692174185	37.0	37.0	37.0	33.0	37.0
25	35.38699094135958	37.0	37.0	37.0	33.0	37.0
26	35.263036492100284	37.0	37.0	37.0	33.0	37.0
27	35.27894292222956	37.0	37.0	37.0	33.0	37.0
28	35.30771881761166	37.0	37.0	37.0	33.0	37.0
29	35.30483115047595	37.0	37.0	37.0	33.0	37.0
30	35.301326386011795	37.0	37.0	37.0	33.0	37.0
31	35.28374657703826	37.0	37.0	37.0	33.0	37.0
32	35.27296479186029	37.0	37.0	37.0	33.0	37.0
33	35.2688902076856	37.0	37.0	37.0	33.0	37.0
34	35.25075675292344	37.0	37.0	37.0	33.0	37.0
35	35.19333684183024	37.0	37.0	37.0	33.0	37.0
36	35.221218194915664	37.0	37.0	37.0	33.0	37.0
37	35.20657581899142	37.0	37.0	37.0	33.0	37.0
38	35.19487212026431	37.0	37.0	37.0	33.0	37.0
39	35.08798240277296	37.0	37.0	37.0	33.0	37.0
40	34.877630595088355	37.0	37.0	37.0	27.0	37.0
41	35.05662614477153	37.0	37.0	37.0	27.0	37.0
42	35.07894118042259	37.0	37.0	37.0	33.0	37.0
43	34.7406262791395	37.0	37.0	37.0	27.0	37.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
14	4.0
15	30.0
16	48.0
17	37.0
18	21.0
19	31.0
20	80.0
21	233.0
22	547.0
23	1157.0
24	2315.0
25	3963.0
26	6476.0
27	9446.0
28	12844.0
29	17105.0
30	21801.0
31	27423.0
32	34936.0
33	45703.0
34	65562.0
35	131253.0
36	422748.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	41.093954312403035	20.66168758701259	13.714241635905111	24.530116464679264
2	16.258897212237937	21.40456826203744	38.962729063169114	23.373805462555506
3	17.595609651103622	27.628293414849896	29.973885336846806	24.802211597199673
4	12.428663673246964	18.0849329964181	39.9111429612958	29.575260369039132
5	13.637228884633904	37.36287935622814	35.43556993790458	13.564321821233374
6	29.143789898266032	40.30852378126388	17.232816141076412	13.314870179393676
7	26.67863536888361	32.25254210507326	23.611188870351086	17.45763365569204
8	24.579260304343446	35.77124102502852	20.447942988169398	19.201555682458636
9	26.20822307073105	15.445722184275713	20.276375001088628	38.06967974390461
10	15.464508816653666	27.790157048781793	33.67796228490239	23.067371849662152
11	33.82713561087037	23.83973882848551	23.975599772569776	18.357525788074344
12	23.315952588014127	26.12561165418164	29.556473736661182	21.001962021143047
13	27.681418527600798	22.338425630440813	26.05755676735555	23.92259907460284
14	22.15354526147633	20.986410173148055	27.920170498019935	28.939874067355674
15	24.190588519252564	28.32215466499453	24.970669214681443	22.51658760107146
16	22.8140633495197	28.30013324823362	25.252966359486567	23.632837042760116
17	22.454504623875447	27.39277622881372	26.309496704874448	23.84322244243639
18	23.04958053555588	27.09977941258804	27.745367726556214	22.10527232529987
19	25.041461226754652	25.927418903333443	27.573177665555644	21.457942204356257
20	24.187353734869607	26.15559561711599	27.842162428477053	21.81488821953735
21	23.173622075163948	26.92286158979699	27.675944277106563	22.2275720579325
22	22.934372445608968	26.677888880179857	27.502634483050354	22.885104191160828
23	22.721249920685572	26.749800625308705	28.195624829707267	22.333324624298456
24	23.086158482040105	27.362170191959574	26.508187114858483	23.04348421114184
25	23.04684341030876	26.747187914845544	27.886080847214913	22.319887827630783
26	23.1386615208712	26.97449372514037	27.86231762347856	22.024527130509863
27	23.10158591525114	26.89810304778896	26.817109023431033	23.183202013528863
28	22.449154788165167	26.60398649850764	28.213416143813536	22.733442569513652
29	24.235377841478147	26.277522105396738	27.182141999569527	22.304958053555588
30	23.476074414970583	26.25114617119723	27.704062018281505	22.568717395550678
31	22.991852075798462	26.537549003873036	27.832209245760254	22.63838967456825
32	22.130901770795617	26.285235822002257	27.97752571342548	23.606336693776647
33	22.111493064497868	26.61866744301492	28.268283063539872	23.00155642894734
34	23.183948502232624	26.341098060000274	28.06187893695032	22.413074500816784
35	23.057418666945356	27.064445613943416	27.279061116274324	22.599074602836904
36	23.91700040932464	26.68261664197033	26.897978633005003	22.502404315700026
37	22.956642691937798	26.323057916326082	27.295981526892877	23.424317864843243
38	23.282982670264744	26.66208820261694	27.22008850867731	22.834840618441007
39	22.523181584621337	26.786129742225008	27.61099975987947	23.07968891327419
40	22.87166739449315	27.13822358083166	27.783189820880033	22.20691920379515
41	22.64162445895121	26.159950134554588	27.730562367264977	23.467863039229226
42	22.25369916256409	27.416041793414227	27.43594815884782	22.894310885173862
43	23.43103626317708	25.762942558938395	27.48210604369696	23.323915134187565
>>END_MODULE
>>Per sequence GC content	pass
#GC Content	Count
0	37.0
1	39.0
2	41.0
3	182.5
4	324.0
5	324.0
6	409.0
7	494.0
8	480.5
9	467.0
10	634.5
11	802.0
12	802.0
13	1358.0
14	1914.0
15	3431.0
16	4948.0
17	5620.5
18	6293.0
19	6293.0
20	5616.0
21	4939.0
22	5129.0
23	5319.0
24	7162.0
25	9005.0
26	9005.0
27	12036.0
28	15067.0
29	18237.0
30	21407.0
31	25660.0
32	29913.0
33	29913.0
34	37340.5
35	44768.0
36	48689.5
37	52611.0
38	56262.0
39	59913.0
40	59913.0
41	62509.0
42	65105.0
43	66106.5
44	67108.0
45	65377.0
46	63646.0
47	63646.0
48	60689.0
49	57732.0
50	58101.5
51	58471.0
52	55291.5
53	52112.0
54	52112.0
55	50968.5
56	49825.0
57	42518.0
58	35211.0
59	32165.0
60	29119.0
61	29119.0
62	25975.0
63	22831.0
64	18445.0
65	14059.0
66	11832.5
67	9606.0
68	9606.0
69	8078.0
70	6550.0
71	5604.5
72	4659.0
73	4442.5
74	4226.0
75	4226.0
76	3458.5
77	2691.0
78	2058.0
79	1425.0
80	956.5
81	488.0
82	488.0
83	379.5
84	271.0
85	232.5
86	194.0
87	149.5
88	105.0
89	105.0
90	78.0
91	51.0
92	32.0
93	13.0
94	8.0
95	3.0
96	3.0
97	1.5
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
43	803763.0
>>END_MODULE
>>Sequence Duplication Levels	warn
#Total Deduplicated Percentage	52.67074632491975
#Duplication Level	Percentage of deduplicated	Percentage of total
1	86.48944138893364	45.554634271805384
2	7.595368189757282	8.001074223341414
3	1.9225492007866083	3.0378630375542595
4	0.8986200354393249	1.8932395171646028
5	0.5368747982514818	1.413879815347313
6	0.38048516469373167	1.202426255398731
7	0.2669837631733825	0.9843563844084375
8	0.19758187291643803	0.8325427765427391
9	0.16344184331639022	0.7747743481375381
>10	1.2474104187547863	13.19280234200165
>50	0.16562398084696497	6.127334673876375
>100	0.12505783676245139	12.172597259013228
>500	0.0086412324825164	3.0635990151565573
>1k	0.0019202738850036447	1.7488760802517604
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT	3926	0.48845244182675734	No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT	2772	0.344877781136977	No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT	1577	0.1962021143048386	No Hit
GTATTAGAGGCACCGCCTGCCCAGTGACACATGTTTAACGGCC	1310	0.16298336698753238	No Hit
TTATTACCCTCACTGTCAACCCAACACAGGCATGCTCATAAGG	1127	0.14021546152286182	No Hit
GTACATGCTAAGACTTCACCAGTCAAAGCGAACTACTATACTC	1060	0.1318796709975453	No Hit
GTATGAATGGCTCCACGAGGGTTCAGCTGTCTCTTACTTTTAA	1045	0.1300134492381461	No Hit
CCCCTATACCTTCTGCATAATGAATTAACTAGAAATAACTTTG	1016	0.12640542050330758	No Hit
AGCATGTACTGCTCGGAGGTTGGGTTCTGCTCCGAGGTCGCCC	996	0.12391712482410859	No Hit
CTCCAGGACAGGCTGCATCAGAAGAGGCCATCAAGCAGATCAC	908	0.11296862383563314	No Hit
CTCTAATACTGGTGATGCTAGAGGTGATGTTTTTGGTAAACAG	894	0.11122681686019387	No Hit
TGCTAAGACTTCACCAGTCAAAGCGAACTACTATACTCAATTG	816	0.1015224637113179	No Hit
CCATAGGGTCTTCTCGTCTTGCTGTGTCATGCCCGCCTCTTCA	806	0.10027831587171841	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	2.4882956791989676E-4	1.2441478395994838E-4	1.2441478395994838E-4
2	0.0	0.0	3.732443518798452E-4	1.2441478395994838E-4	1.2441478395994838E-4
3	0.0	0.0	3.732443518798452E-4	2.4882956791989676E-4	1.2441478395994838E-4
4	0.0	0.0	4.976591358397935E-4	3.732443518798452E-4	1.2441478395994838E-4
5	0.0	0.0	7.464887037596904E-4	3.732443518798452E-4	1.2441478395994838E-4
6	0.0	0.0	0.0013685626235594324	3.732443518798452E-4	1.2441478395994838E-4
7	0.0	0.0	0.0029859548150387616	3.732443518798452E-4	1.2441478395994838E-4
8	0.0	0.0	0.0036080287348385035	3.732443518798452E-4	1.2441478395994838E-4
9	0.0	0.0	0.004603347006518091	4.976591358397935E-4	1.2441478395994838E-4
10	4.976591358397935E-4	0.0	0.005349835710277781	7.464887037596904E-4	1.2441478395994838E-4
11	4.976591358397935E-4	0.0	0.008957864445116284	0.0013685626235594324	1.2441478395994838E-4
12	4.976591358397935E-4	0.0	0.009206694013036181	0.0014929774075193808	1.2441478395994838E-4
13	4.976591358397935E-4	0.0	0.009455523580956077	0.0014929774075193808	1.2441478395994838E-4
14	4.976591358397935E-4	0.0	0.009579938364916026	0.0014929774075193808	1.2441478395994838E-4
15	4.976591358397935E-4	0.0	0.010202012284715768	0.0014929774075193808	1.2441478395994838E-4
16	6.220739197997419E-4	0.0	0.011819404476195098	0.0014929774075193808	2.4882956791989676E-4
17	6.220739197997419E-4	0.0	0.011819404476195098	0.0014929774075193808	2.4882956791989676E-4
18	6.220739197997419E-4	0.0	0.012068234044114994	0.0016173921914793292	2.4882956791989676E-4
19	6.220739197997419E-4	0.0	0.012317063612034891	0.0016173921914793292	2.4882956791989676E-4
20	6.220739197997419E-4	0.0	0.012690307963914736	0.0016173921914793292	2.4882956791989676E-4
21	6.220739197997419E-4	0.0	0.01318796709975453	0.0016173921914793292	2.4882956791989676E-4
22	6.220739197997419E-4	0.0	0.013312381883714478	0.001990636543359174	2.4882956791989676E-4
23	6.220739197997419E-4	0.0	0.013436796667674427	0.0023638808952390193	2.4882956791989676E-4
24	6.220739197997419E-4	0.0	0.013436796667674427	0.0026127104631589165	2.4882956791989676E-4
25	6.220739197997419E-4	0.0	0.013436796667674427	0.002737125247118865	2.4882956791989676E-4
26	6.220739197997419E-4	0.0	0.013561211451634374	0.0033591991669186068	2.4882956791989676E-4
27	6.220739197997419E-4	0.0	0.013561211451634374	0.004478932222558142	2.4882956791989676E-4
28	6.220739197997419E-4	0.0	0.013561211451634374	0.012192648828074942	2.4882956791989676E-4
29	6.220739197997419E-4	0.0	0.013561211451634374	0.029610718582467718	2.4882956791989676E-4
30	6.220739197997419E-4	0.0	0.013561211451634374	0.05635989713385662	2.4882956791989676E-4
31	6.220739197997419E-4	0.0	0.013561211451634374	0.11458601602711246	2.4882956791989676E-4
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
ATACCGT	25	0.005495373	29.599998	6
CGAGTCG	95	1.0913936E-11	25.31579	21
GTATCAA	2170	0.0	23.018433	1
GGTATCA	805	0.0	22.291925	1
AGTCGCC	105	9.822543E-10	21.142859	23
TTAACGG	370	0.0	21.0	35
TACTAGG	45	0.0038248224	20.555555	2
TCGGCGT	55	5.141246E-4	20.181818	11
CCGAGTT	110	1.7516868E-9	20.181818	13
TAACGGC	395	0.0	20.13924	36
AACGGCC	385	0.0	19.701298	37
GCTCGGA	275	0.0	19.509092	11
GTCGCCG	115	3.0468073E-9	19.304348	24
GCCGTCC	115	3.0468073E-9	19.304348	27
TCGCCGT	125	4.110916E-10	19.24	25
GTCCGGC	125	4.110916E-10	19.24	15
GTCGCCC	260	0.0	19.211538	37
TTTAACG	405	0.0	19.185184	34
CTGCTCG	500	0.0	18.869999	9
CGGCGTA	60	9.233524E-4	18.5	12
>>END_MODULE