FastQCFastQC Report
Fri 10 Feb 2017
ERR1633294.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1633294.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences992841
Sequences flagged as poor quality0
Sequence length43
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT70510.7101842087504444No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT51140.5150875114947913No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT29560.2977314595186943No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT12280.1236854642384833No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA13600.025.0294111
CGGATTT451.3230351E-424.66666613
GTATCAA34850.022.6140611
GCCGCTC2500.021.4627
AACGGCC2000.021.27499837
CCGCTCT2450.021.14285728
TTCGCCG2450.021.14285724
GACCGTT759.263684E-619.7333347
CGCTCTC2700.019.18518629
GTATTGG1650.019.0606061
TTAACGG2200.018.535
TATCGCC609.2356047E-418.531
ATACCGC609.2356047E-418.56
GTCGCCC1653.6379788E-1217.93939437
GTTCTAT1156.402297E-817.6956521
CTTAGAC1054.795638E-717.6190473
TGCTCGC3050.017.59016210
GCCGGCA2850.017.52631615
CGGCAAT752.0669083E-417.26666626
CCGTTTA752.0669083E-417.26666627
Produced by FastQC (version 0.11.5)