##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	ERR1633294.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	992841
Sequences flagged as poor quality	0
Sequence length	43
%GC	45
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.53793306279656	33.0	33.0	33.0	27.0	33.0
2	31.74071376987856	33.0	33.0	33.0	27.0	33.0
3	31.47171097889793	33.0	33.0	33.0	27.0	33.0
4	31.76715607030733	33.0	33.0	33.0	27.0	33.0
5	31.876922890976502	33.0	33.0	33.0	33.0	33.0
6	34.96545670454786	37.0	37.0	37.0	27.0	37.0
7	35.13725964177547	37.0	37.0	37.0	33.0	37.0
8	35.2632919067605	37.0	37.0	37.0	33.0	37.0
9	35.38659765259493	37.0	37.0	37.0	33.0	37.0
10	35.39640083356751	37.0	37.0	37.0	33.0	37.0
11	35.431998678539664	37.0	37.0	37.0	33.0	37.0
12	35.40454715306882	37.0	37.0	37.0	33.0	37.0
13	35.40537004414604	37.0	37.0	37.0	33.0	37.0
14	35.39434914553287	37.0	37.0	37.0	33.0	37.0
15	35.410348686244824	37.0	37.0	37.0	33.0	37.0
16	35.40537608740976	37.0	37.0	37.0	33.0	37.0
17	35.38020690120573	37.0	37.0	37.0	33.0	37.0
18	35.36252834038884	37.0	37.0	37.0	33.0	37.0
19	35.34489409683927	37.0	37.0	37.0	33.0	37.0
20	35.350602966638164	37.0	37.0	37.0	33.0	37.0
21	35.34239420007836	37.0	37.0	37.0	33.0	37.0
22	35.248443607788154	37.0	37.0	37.0	33.0	37.0
23	35.29982242876755	37.0	37.0	37.0	33.0	37.0
24	35.279590589026846	37.0	37.0	37.0	33.0	37.0
25	35.30361558396561	37.0	37.0	37.0	33.0	37.0
26	35.18186396411913	37.0	37.0	37.0	33.0	37.0
27	35.19718363766202	37.0	37.0	37.0	33.0	37.0
28	35.20917850894554	37.0	37.0	37.0	33.0	37.0
29	35.21597214458307	37.0	37.0	37.0	33.0	37.0
30	35.22725391074704	37.0	37.0	37.0	33.0	37.0
31	35.200986864966296	37.0	37.0	37.0	33.0	37.0
32	35.18860522480438	37.0	37.0	37.0	33.0	37.0
33	35.17700115124174	37.0	37.0	37.0	33.0	37.0
34	35.14131366452433	37.0	37.0	37.0	33.0	37.0
35	35.0855141961301	37.0	37.0	37.0	33.0	37.0
36	35.09714143553701	37.0	37.0	37.0	33.0	37.0
37	35.092301788503896	37.0	37.0	37.0	33.0	37.0
38	35.067348145372726	37.0	37.0	37.0	33.0	37.0
39	34.94709223329818	37.0	37.0	37.0	27.0	37.0
40	34.72074078326741	37.0	37.0	37.0	27.0	37.0
41	34.89372316413202	37.0	37.0	37.0	27.0	37.0
42	34.919675960199065	37.0	37.0	37.0	27.0	37.0
43	34.549335694235026	37.0	37.0	37.0	27.0	37.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
14	3.0
15	37.0
16	62.0
17	53.0
18	41.0
19	44.0
20	106.0
21	267.0
22	721.0
23	1621.0
24	3067.0
25	5464.0
26	8365.0
27	12304.0
28	16925.0
29	22054.0
30	28669.0
31	36396.0
32	45886.0
33	59346.0
34	84731.0
35	166870.0
36	499809.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	41.44238604167233	20.60793218652332	14.354866489196155	23.59481528260819
2	15.802933198769994	22.597374604795732	39.30095554071599	22.298736655718287
3	17.653984877739738	27.930252678928447	31.20368719664075	23.21207524669106
4	12.281926310456559	18.371219560836025	40.00398855405851	29.34286557464891
5	13.54758717659726	37.390075550868666	35.13734827631011	13.924988996223966
6	27.99501632184811	40.92065094008003	17.3893906476465	13.694942090425355
7	25.408700889669138	33.56962494498112	23.35107031236623	17.67060385298351
8	24.295632432584874	35.476979697655516	20.83143222328651	19.3959556464731
9	26.3501406569632	15.381012669702399	20.33507882933924	37.933767843995156
10	15.035539426756147	28.186990666179177	33.689382287798345	23.08808761926633
11	33.05786122853508	24.2747831727336	24.212537556366023	18.454818042365293
12	22.8313496320156	26.623497619457698	30.119626405436524	20.425526343090183
13	28.015261255326884	22.837695058926858	26.727743918714076	22.419299767032182
14	22.137180072136424	21.841966639169815	28.682941175878113	27.337912112815648
15	23.86585566067477	28.634997950326387	25.795066883821278	21.704079505177567
16	21.93180982654826	29.116041742836966	25.771195992107497	23.180952438507273
17	21.99476049035042	27.563930176130917	26.650490864096067	23.790818469422597
18	22.9969350580808	26.630145209555206	28.628249639166796	21.7446700931972
19	24.938333529739403	25.969415042287736	28.830094647582037	20.262156780390818
20	24.661048445823653	25.688403279074894	28.63570299776097	21.01484527734048
21	22.538855667725244	27.29168114531934	28.148716662587464	22.02074652436795
22	22.15510842118728	27.28231408654558	28.17248683323916	22.39009065902798
23	22.052876543172573	26.897156745138446	29.01924880217477	22.030717909514212
24	22.629202460414106	27.14160676281499	28.080427782494883	22.148762994276023
25	22.664857716391648	26.740031888288257	28.88861358465253	21.70649681066757
26	22.555474642969013	27.02295735168068	28.4108935871907	22.010674418159603
27	22.53704268860774	26.891314923537607	28.212070210637958	22.359572177216695
28	21.771965501021814	27.065864524128237	28.783662237961565	22.378507736888384
29	23.07217369145714	26.960711735313108	28.0413480104065	21.925766562823252
30	22.569676312722784	27.106354391085784	28.50688075935623	21.817088536835204
31	22.581158513800297	26.82876714398378	28.50214686943831	22.087927472777615
32	21.477759278676043	26.78626285578456	28.73924424958276	22.996733615956632
33	21.622696887014133	26.754233558042024	29.306605992298866	22.316463562644977
34	22.094877226061374	26.691585057426114	28.59893981010051	22.614597906412005
35	22.144331267544352	27.379107027207784	28.383799621490247	22.092762083757624
36	22.667879348254154	27.038871279489868	28.284186491089713	22.00906288116627
37	22.851090960183956	26.524589536491742	28.095133057559064	22.529186445765234
38	22.650555325575798	26.962021109120194	28.094025125876147	22.293398439427865
39	22.28312489109535	26.689067030874025	28.342101101787698	22.685706976242923
40	22.22158432216236	27.283220576104334	28.544348994451273	21.950846107282032
41	22.2426350241378	26.555208739365117	28.58141434529799	22.620741891199096
42	21.961119655614546	27.10303059603703	28.40595825514861	22.529891493199816
43	22.269225384527836	26.636188473280214	28.281970627723872	22.812615514468078
>>END_MODULE
>>Per sequence GC content	pass
#GC Content	Count
0	52.0
1	68.0
2	84.0
3	361.0
4	638.0
5	638.0
6	816.5
7	995.0
8	968.5
9	942.0
10	1364.0
11	1786.0
12	1786.0
13	2711.5
14	3637.0
15	6772.0
16	9907.0
17	10997.0
18	12087.0
19	12087.0
20	11178.5
21	10270.0
22	10831.0
23	11392.0
24	14315.5
25	17239.0
26	17239.0
27	20765.5
28	24292.0
29	29739.0
30	35186.0
31	38685.0
32	42184.0
33	42184.0
34	47247.5
35	52311.0
36	55519.0
37	58727.0
38	62417.0
39	66107.0
40	66107.0
41	69456.5
42	72806.0
43	71823.0
44	70840.0
45	71696.0
46	72552.0
47	72552.0
48	71356.0
49	70160.0
50	72151.5
51	74143.0
52	73102.5
53	72062.0
54	72062.0
55	65509.5
56	58957.0
57	51564.5
58	44172.0
59	39570.0
60	34968.0
61	34968.0
62	30385.5
63	25803.0
64	20887.0
65	15971.0
66	13756.0
67	11541.0
68	11541.0
69	9633.5
70	7726.0
71	6629.5
72	5533.0
73	4543.0
74	3553.0
75	3553.0
76	2582.0
77	1611.0
78	1232.0
79	853.0
80	689.0
81	525.0
82	525.0
83	484.0
84	443.0
85	409.0
86	375.0
87	308.0
88	241.0
89	241.0
90	178.0
91	115.0
92	80.5
93	46.0
94	27.5
95	9.0
96	9.0
97	4.5
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
43	992841.0
>>END_MODULE
>>Sequence Duplication Levels	warn
#Total Deduplicated Percentage	53.60640465986225
#Duplication Level	Percentage of deduplicated	Percentage of total
1	86.70829529336851	46.48119964863142
2	7.48686061991499	8.026873600463002
3	1.907493303289925	3.0676157370641124
4	0.9016473188015341	1.9333628412861945
5	0.5438596948008484	1.4577181438841729
6	0.36176005639060865	1.1635593583589734
7	0.265336058499233	0.9956598485933936
8	0.1913451280429108	0.8205859490849146
9	0.16378095288283184	0.7901737232233426
>10	1.1610411775502234	12.727306390029078
>50	0.17905603476417017	6.744451736367452
>100	0.1224767126318637	11.971331196287323
>500	0.006285741055763267	2.1508002223337264
>1k	3.80954003379592E-4	0.4272193377442229
>5k	3.80954003379592E-4	1.242142266648774
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT	7051	0.7101842087504444	No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT	5114	0.5150875114947913	No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT	2956	0.2977314595186943	No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	1228	0.1236854642384833	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	1.0072106208345546E-4	0.0	4.028842483338218E-4	0.0	0.0
2	2.014421241669109E-4	0.0	5.036053104172772E-4	0.0	0.0
3	2.014421241669109E-4	0.0	6.043263725007327E-4	0.0	0.0
4	2.014421241669109E-4	0.0	0.00110793168291801	1.0072106208345546E-4	0.0
5	3.0216318625036635E-4	0.0	0.0017122580554187429	1.0072106208345546E-4	0.0
6	3.0216318625036635E-4	0.0	0.0034245161108374857	1.0072106208345546E-4	0.0
7	3.0216318625036635E-4	0.0	0.006345426911257694	2.014421241669109E-4	0.0
8	3.0216318625036635E-4	0.0	0.007453358594175704	2.014421241669109E-4	0.0
9	3.0216318625036635E-4	0.0	0.009064895587510991	7.050474345841882E-4	0.0
10	6.043263725007327E-4	0.0	0.00976994302209518	7.050474345841882E-4	0.0
11	6.043263725007327E-4	0.0	0.014906717188351407	7.050474345841882E-4	0.0
12	6.043263725007327E-4	0.0	0.015913927809185963	7.050474345841882E-4	0.0
13	6.043263725007327E-4	0.0	0.017626185864604706	7.050474345841882E-4	0.0
14	6.043263725007327E-4	0.0	0.018734117547522716	7.050474345841882E-4	0.0
15	6.043263725007327E-4	0.0	0.01994277029252418	9.064895587510991E-4	0.0
16	6.043263725007327E-4	0.0	0.021151423037525645	0.0012086527450014654	0.0
17	7.050474345841882E-4	0.0	0.021453586223776012	0.001309373807084921	0.0
18	7.050474345841882E-4	0.0	0.022460796844610567	0.0014100948691683764	0.0
19	7.050474345841882E-4	0.0	0.023165844279194756	0.0014100948691683764	0.0
20	7.050474345841882E-4	0.0	0.023367286403361665	0.001510815931251832	0.0
21	7.050474345841882E-4	0.0	0.0239716127758624	0.0021151423037525647	0.0
22	7.050474345841882E-4	0.0	0.024072333837945853	0.002920910800420208	0.0
23	7.050474345841882E-4	0.0	0.024273775962112765	0.00332379504875403	0.0
24	7.050474345841882E-4	0.0	0.024676660210446587	0.00443172673167204	0.0
25	7.050474345841882E-4	0.0	0.024676660210446587	0.004633168855838951	0.0
26	7.050474345841882E-4	0.0	0.024676660210446587	0.005237495228339684	0.0
27	7.050474345841882E-4	0.0	0.024676660210446587	0.0065468690354246045	0.0
28	7.050474345841882E-4	0.0	0.024676660210446587	0.015913927809185963	0.0
29	7.050474345841882E-4	0.0	0.024676660210446587	0.04038914589546564	0.0
30	7.050474345841882E-4	0.0	0.024676660210446587	0.07201555938967065	0.0
31	7.050474345841882E-4	0.0	0.024676660210446587	0.14312462922059022	0.0
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GGTATCA	1360	0.0	25.029411	1
CGGATTT	45	1.3230351E-4	24.666666	13
GTATCAA	3485	0.0	22.614061	1
GCCGCTC	250	0.0	21.46	27
AACGGCC	200	0.0	21.274998	37
CCGCTCT	245	0.0	21.142857	28
TTCGCCG	245	0.0	21.142857	24
GACCGTT	75	9.263684E-6	19.733334	7
CGCTCTC	270	0.0	19.185186	29
GTATTGG	165	0.0	19.060606	1
TTAACGG	220	0.0	18.5	35
TATCGCC	60	9.2356047E-4	18.5	31
ATACCGC	60	9.2356047E-4	18.5	6
GTCGCCC	165	3.6379788E-12	17.939394	37
GTTCTAT	115	6.402297E-8	17.695652	1
CTTAGAC	105	4.795638E-7	17.619047	3
TGCTCGC	305	0.0	17.590162	10
GCCGGCA	285	0.0	17.526316	15
CGGCAAT	75	2.0669083E-4	17.266666	26
CCGTTTA	75	2.0669083E-4	17.266666	27
>>END_MODULE