FastQCFastQC Report
Fri 10 Feb 2017
ERR1633293.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1633293.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences485717
Sequences flagged as poor quality0
Sequence length43
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT138642.854336990469759No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT113862.344163370851751No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT66741.3740511450083073No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT25000.5147030060714366No Hit
ATCATTAACTGAATCCATAGGTTAATGAGGCGAACCGGGGGAA23490.4836149445047219No Hit
GTATGAAACTGCTCGCCGGCAGCTTCGCCGCTCTCTTCCTGAG21630.445321040853007No Hit
TCTTTAGTGACCATGAAGTGCGATTGCCTCGGTGATAACGACA18600.3829390365171489No Hit
GTTAATGATAGTGTGTCGAAACACACTGGGTTTCCCCATTCGG17760.3656450155131486No Hit
GTACTGGTTCACTATCGGTCAGTCAGGAGTATTTAGCCTTGGA17250.3551450741892913No Hit
ATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTT16470.33908634039986246No Hit
ACCATGAAGTGCGATTGCCTCGGTGATAACGACATTGGGGCCA16440.33846869679257674No Hit
GTATGAAACTGCTTGTCCGGCGAGTCGCCGTCCGCCGCCTGGG14910.3069688728210048No Hit
ATACAGGGTGACAGCCCCGTACACAAAAATGCACATGCTGTGA14460.297704218711719No Hit
CCGTATTACCGCGGCTGCTGGCACGGAGTTAGCCGGTGCTTCT14090.29008661422186166No Hit
TCTTTAGTGGCCCCAATGTCGTTATCACCGAGGCAATCGCACT13220.2721749496105757No Hit
CATCTTTAGTGACCATGAAGTGCGATTGCCTCGGTGATAACGA12260.2524103541774325No Hit
GTTAATACCTTTGCTCATTGACGTTACCCGCAGAAGAAGCACC11860.24417510608028956No Hit
TATTCAGACAGGATACCACGTGTCCCGCCCTACTCATCGAGCT10810.22255757982528923No Hit
TCATTAACCTATGGATTCAGTTAATGATAGTGTGTCGAAACAC10620.2186458369791463No Hit
CCGTCATCTTTAGTGACCATGAAGTGCGATTGCCTCGGTGATA10010.20608708363100323No Hit
GTCATCTTTAGTGGCCCCAATGTCGTTATCACCGAGGCAATCG9680.1992930039508603No Hit
GGATACCACGTGTCCCGCCCTACTCATCGAGCTCACAGCATGT9530.19620478591443166No Hit
CCCCAATGTCGTTATCACCGAGGCAATCGCACTTCATGGTCAC9450.19455773629500306No Hit
GTGGATGCCCTGGCAGTCAGAGGCGATGAAGGACGTGCTAATC9370.19291068667557446No Hit
GAGTTAGCCGGTGCTTCTTCTGCGGGTAACGTCAATGAGCAAA9030.1859107257930029No Hit
CCCATATTCAGACAGGATACCACGTGTCCCGCCCTACTCATCG8900.18323427016143146No Hit
GGTTAATGAGGCGAACCGGGGGAACTGAAACATCTAAGTACCC8780.18076369573228857No Hit
AGCGTACACGGTGGATGCCCTGGCAGTCAGAGGCGATGAAGGA8700.17911664611285996No Hit
CGTTATCACCGAGGCAATCGCACTTCATGGTCACTAAAGATGA8680.17870488370800283No Hit
CATCTTTAGTGGCCCCAATGTCGTTATCACCGAGGCAATCGCA8670.17849900250557424No Hit
TAATGATAGTGTGTCGAAACACACTGGGTTTCCCCATTCGGAA8450.1739696160521456No Hit
GTAATACGGAGGGTGCAAGCGTTAATCGGAATTACTGGGCGTA8330.1714990416230027No Hit
GGTATTAACTTTACTCCCTTCCTCCCCGCTGAAAGTACTTTAC8310.17108727921814554No Hit
GGTGATATGAACCGTTATAACCGGCGATTTCCGAATGGGGAAA8300.17088139801571697No Hit
ATGATAGTGTGTCGAAACACACTGGGTTTCCCCATTCGGAAAT8150.16779317997928836No Hit
GTATCAACGCAGAGTACGGGGATTTCACATCTGACTTAACAAA7860.1618226251088597No Hit
TCACGGTACTGGTTCACTATCGGTCAGTCAGGAGTATTTAGCC7830.16120498150157395No Hit
CCGTCATCTTTAGTGGCCCCAATGTCGTTATCACCGAGGCAAT7830.16120498150157395No Hit
CCTATGGATTCAGTTAATGATAGTGTGTCGAAACACACTGGGT7760.15976381308457394No Hit
GCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT7730.1591461694772882No Hit
GTATTTAGCCTTGGAGGATGGTCCCCCCATATTCAGACAGGAT7640.15729323865543104No Hit
CTATCGGTCAGTCAGGAGTATTTAGCCTTGGAGGATGGTCCCC7550.15544030783357388No Hit
GATATGAACCGTTATAACCGGCGATTTCCGAATGGGGAAACCC7490.1542050206190024No Hit
GTCAGGAGTATTTAGCCTTGGAGGATGGTCCCCCCATATTCAG7270.14967563416557378No Hit
GTCGTTATCACCGAGGCAATCGCACTTCATGGTCACTAAAGAT7120.14658741612914516No Hit
GTCATCTTTAGTGACCATGAAGTGCGATTGCCTCGGTGATAAC7010.14432272290243084No Hit
ACCCTGTATCGCGCGCCTTTCCAGACGCTTCCACTAACACACA7010.14432272290243084No Hit
CGGTACTGGTTCACTATCGGTCAGTCAGGAGTATTTAGCCTTG6890.14185214847328792No Hit
TATCAACGCAGAGTACGGGGATTTCACATCTGACTTAACAAAC6850.14102862366357363No Hit
GTGCTAATCTGCGATAAGCGTCGGTAAGGTGATATGAACCGTT6750.1389698116392879No Hit
GTGGTATCCTGTCTGAATATGGGGGGACCATCCTCCAAGGCTA6700.13794040562714502No Hit
GTATCAACGCAGAGTACGGGCTCCGTCATCTTTAGTGACCATG6610.13608747480528785No Hit
GAATATGGGGGGACCATCCTCCAAGGCTAAATACTCCTGACTG6580.13546983119800213No Hit
GTTATAACCGGCGATTTCCGAATGGGGAAACCCAGTGTGTTTC6240.12846987031543058No Hit
GTATCAACGCAGAGTACGGGACTAGTATGAAACTGCTTGTCCG6240.12846987031543058No Hit
CAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT6200.1276463455057163No Hit
GTCTGAATATGGGGGGACCATCCTCCAAGGCTAAATACTCCTG6000.12352872145714479No Hit
TTAGTGGCCCCAATGTCGTTATCACCGAGGCAATCGCACTTCA5990.12332284025471624No Hit
ATCTTTAGTGGCCCCAATGTCGTTATCACCGAGGCAATCGCAC5900.12146990943285906No Hit
GTGTGTGTGTTAGTGGAAGCGTCTGGAAAGGCGCGCGATACAG5840.1202346222182876No Hit
GTATTACCGCGGCTGCTGGCACGGAGTTAGCCGGTGCTTCTTC5770.11879345380128759No Hit
GTTCACTATCGGTCAGTCAGGAGTATTTAGCCTTGGAGGATGG5750.11838169139643043No Hit
ATATTGCACAATGGGCGCAAGCCTGATGCAGCCATGCCGCGTG5680.11694052297943042No Hit
TCTAAGTACCCCGAGGAAAAGAAATCAACCGAGATTCCCCCAG5620.11570523576485896No Hit
CCGTTATAACCGGCGATTTCCGAATGGGGAAACCCAGTGTGTT5460.11241113652600176No Hit
CACTATCATTAACTGAATCCATAGGTTAATGAGGCGAACCGGG5390.11096996810900175No Hit
CTATCATTAACTGAATCCATAGGTTAATGAGGCGAACCGGGGG5380.11076408690657318No Hit
TCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTT5380.11076408690657318No Hit
AGTTAATACCTTTGCTCATTGACGTTACCCGCAGAAGAAGCAC5380.11076408690657318No Hit
CGCTTGCACCCTCCGTATTACCGCGGCTGCTGGCACGGAGTTA5370.1105582057041446No Hit
CTTTACGCCCAGTAATTCCGATTAACGCTTGCACCCTCCGTAT5330.1097346808944303No Hit
GCCTGAATCAGTGTGTGTGTTAGTGGAAGCGTCTGGAAAGGCG5310.10932291848957315No Hit
CATTAACCTATGGATTCAGTTAATGATAGTGTGTCGAAACACA5280.10870527488228741No Hit
GAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT5250.1080876312750017No Hit
TATCAACGCAGAGTACGGGCTCCGTCATCTTTAGTGACCATGA5250.1080876312750017No Hit
GGAGTAAAGTTAATACCTTTGCTCATTGACGTTACCCGCAGAA5220.10746998766771598No Hit
CGTGGTATCCTGTCTGAATATGGGGGGACCATCCTCCAAGGCT5210.1072641064652874No Hit
TGTATGAAGAAGGCCTTCGGGTTGTAAAGTACTTTCAGCGGGG5180.10664646285800168No Hit
GCCTTGGAGGATGGTCCCCCCATATTCAGACAGGATACCACGT5080.10458765083371593No Hit
GTATGAAGAAGGCCTTCGGGTTGTAAAGTACTTTCAGCGGGGA5060.10417588842885878No Hit
GGCTAACTCCGTGCCAGCAGCCGCGGTAATACGGAGGGTGCAA5040.10376412602400163No Hit
GTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT5030.10355824482157305No Hit
TCGGTAAGGTGATATGAACCGTTATAACCGGCGATTTCCGAAT5010.10314648241671592No Hit
CTCCGTATTACCGCGGCTGCTGGCACGGAGTTAGCCGGTGCTT5010.10314648241671592No Hit
GGAGTATTTAGCCTTGGAGGATGGTCCCCCCATATTCAGACAG5000.10294060121428733No Hit
GGGTTTCCCCATTCGGAAATCGCCGGTTATAACGGTTCATATC5000.10294060121428733No Hit
GGTTCACTATCGGTCAGTCAGGAGTATTTAGCCTTGGAGGATG4970.1023229576070016No Hit
GTAAAGTACTTTCAGCGGGGAGGAAGGGAGTAAAGTTAATACC4940.10170531399971589No Hit
ATCCATAGGTTAATGAGGCGAACCGGGGGAACTGAAACATCTA4940.10170531399971589No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TGTCAAC251.2325763E-437.02
GAATCAA352.38549E-531.7142852
TTCCTGC352.38549E-531.71428536
AGCAACG303.5964462E-430.8333322
TAGCAAC303.5964462E-430.8333321
TACCCTA250.00549325829.612
TAGACCG250.00549325829.65
CCCTAAA250.00549325829.614
CCTAAAC250.00549325829.615
ACCCTAA250.00549325829.613
GGAATCA405.9331753E-527.7500021
TCCTGCT358.8619924E-426.4285737
AGCGACG358.8619924E-426.4285729
TAGCTAG451.3216761E-424.6666665
GACCCAA551.8997282E-523.54545435
GGTATCA26950.023.4768091
GCTCTCT5650.022.5929230
CGCTCTC5700.022.39473729
CCGCTCT5950.022.38655528
GCCGCTC6000.022.227
Produced by FastQC (version 0.11.5)