FastQCFastQC Report
Fri 10 Feb 2017
ERR1633292.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1633292.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences537385
Sequences flagged as poor quality0
Sequence length43
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT24020.4469793537221918No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT18590.34593447900481034No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT11620.21623231016868727No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TGCGAAT401.5979676E-632.37513
AACTGCG604.3137334E-827.75000210
GGTATCA6900.027.079711
ACTGCGA601.3351928E-624.66666811
GCGAATG551.900215E-523.54545414
CTGCGAA705.0935832E-621.14285912
CGCTACA705.0935832E-621.1428592
GTATCAA16000.020.6968771
CCGGCAG1450.020.41379416
GCCGCTC1450.020.41379427
TTCGCCG1450.020.41379424
CCGCTCT1403.6379788E-1219.8214328
CGGCAGC1600.019.6562517
CGAACTA951.6719605E-719.47368429
AGCTTCG1601.8189894E-1218.521
CGTTAGA500.007030125718.525
GCTTCGC1700.018.522
CTTTCCG500.007030125718.521
GCCGGCA1653.6379788E-1217.93939415
CGCTCTC1552.0008883E-1117.90322529
Produced by FastQC (version 0.11.5)