##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	ERR1633292.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	537385
Sequences flagged as poor quality	0
Sequence length	43
%GC	45
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	30.676224680629343	33.0	33.0	33.0	27.0	33.0
2	31.152823394772835	33.0	33.0	33.0	27.0	33.0
3	30.92743191566568	33.0	33.0	33.0	27.0	33.0
4	31.256384156610252	33.0	33.0	33.0	27.0	33.0
5	31.382913553597515	33.0	33.0	33.0	27.0	33.0
6	33.666269062217964	37.0	33.0	37.0	27.0	37.0
7	33.88846729998046	37.0	33.0	37.0	27.0	37.0
8	34.003316058319456	37.0	33.0	37.0	27.0	37.0
9	34.08248276375411	37.0	33.0	37.0	27.0	37.0
10	34.02701415186505	37.0	33.0	37.0	27.0	37.0
11	34.17758218037348	37.0	33.0	37.0	27.0	37.0
12	34.231556519069194	37.0	33.0	37.0	27.0	37.0
13	34.20744903560762	37.0	33.0	37.0	27.0	37.0
14	34.264497520399715	37.0	37.0	37.0	27.0	37.0
15	34.2656586990705	37.0	37.0	37.0	27.0	37.0
16	34.25851298417336	37.0	37.0	37.0	27.0	37.0
17	34.2539017650288	37.0	37.0	37.0	27.0	37.0
18	34.2095052895038	37.0	33.0	37.0	27.0	37.0
19	34.178066004819634	37.0	33.0	37.0	27.0	37.0
20	34.18379374191687	37.0	33.0	37.0	27.0	37.0
21	34.1894116880821	37.0	33.0	37.0	27.0	37.0
22	34.07210845111047	37.0	33.0	37.0	27.0	37.0
23	34.10398503865943	37.0	33.0	37.0	27.0	37.0
24	34.11112331010356	37.0	33.0	37.0	27.0	37.0
25	34.1190003442597	37.0	33.0	37.0	27.0	37.0
26	34.00829200666189	37.0	33.0	37.0	27.0	37.0
27	33.984342696576945	37.0	33.0	37.0	27.0	37.0
28	34.00520297365948	37.0	33.0	37.0	27.0	37.0
29	33.986899522688574	37.0	33.0	37.0	27.0	37.0
30	33.98962940908287	37.0	33.0	37.0	27.0	37.0
31	33.99902118592815	37.0	33.0	37.0	27.0	37.0
32	33.980042241595875	37.0	33.0	37.0	27.0	37.0
33	33.94290313276329	37.0	33.0	37.0	27.0	37.0
34	33.90499734826986	37.0	33.0	37.0	27.0	37.0
35	33.85164081617462	37.0	33.0	37.0	27.0	37.0
36	33.89841733580208	37.0	33.0	37.0	27.0	37.0
37	33.90668701210492	37.0	33.0	37.0	27.0	37.0
38	33.882877266764055	37.0	33.0	37.0	27.0	37.0
39	33.770090344911004	37.0	33.0	37.0	27.0	37.0
40	33.54316179275566	37.0	33.0	37.0	27.0	37.0
41	33.73597699973018	37.0	33.0	37.0	27.0	37.0
42	33.771757678387004	37.0	33.0	37.0	27.0	37.0
43	33.27959470398318	37.0	33.0	37.0	14.0	37.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
14	2.0
15	25.0
16	43.0
17	34.0
18	60.0
19	94.0
20	216.0
21	562.0
22	1309.0
23	2487.0
24	4187.0
25	6269.0
26	9072.0
27	12459.0
28	16313.0
29	20061.0
30	24873.0
31	30617.0
32	38156.0
33	49161.0
34	68961.0
35	111847.0
36	140577.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	40.895447398047956	21.63160490151381	13.629520734668812	23.84342696576942
2	16.427142551429608	23.58067307423914	37.334499474306135	22.65768490002512
3	18.510751137452665	28.12322636471059	30.194181080603293	23.171841417233455
4	13.39095806544656	19.79921285484336	38.57085702057184	28.238972059138234
5	14.633828633102897	37.21745117560037	35.193762386371034	12.954957804925705
6	28.684648808582303	40.7162462666431	17.41656354382798	13.182541380946622
7	25.999423132391115	32.84237557803065	23.65752672664849	17.500674562929746
8	24.616801734324554	35.92154600519181	20.781562566874772	18.680089693608863
9	26.11312187723885	15.672562501744558	20.496106143640034	37.718209477376554
10	14.734501335169384	28.871107306679566	34.048028880597705	22.346362477553335
11	33.16337448942564	24.337300073504096	24.19047796272691	18.30884747434335
12	22.481461149827407	26.384993998716006	30.90112303097407	20.23242182048252
13	28.070191761958373	22.754077616606345	26.57666291392577	22.599067707509512
14	21.99205411390344	21.63179098783926	28.51512416610065	27.86103073215665
15	23.50791332098961	29.17293932655359	24.79711938368209	22.52202796877471
16	22.218707258297123	29.07133619285987	25.791750793192964	22.918205755650046
17	21.856211096327584	28.121923760432466	27.097332452524725	22.924532690715225
18	22.904063194916123	27.364552415865724	28.096988192822653	21.634396196395507
19	24.406710272895594	26.69408338528243	28.220177340268148	20.67902900155382
20	24.138932050578262	26.51153270001954	28.12973938610121	21.219795863300988
21	22.842840793844267	27.323799510592966	27.772825813895064	22.060533881667705
22	22.364412851121635	27.2063790392363	28.03036928831285	22.398838821329214
23	22.22466202071141	27.23373372907692	28.415009723010503	22.126594527201167
24	22.153018785414556	27.548405705406736	28.02236757631865	22.276207932860054
25	22.554406989402384	27.21475292388139	28.37462899038864	21.856211096327584
26	22.50695497641356	27.49611544795631	28.04916400718293	21.947765568447203
27	22.603533779320227	27.260716246266643	27.72295467867544	22.41279529573769
28	22.24568977548685	27.27374228904789	28.334620430417672	22.14594750504759
29	22.722629027605905	27.27020664886441	27.86959070312718	22.137573620402502
30	22.623258929817542	27.251784102645217	28.144068033160586	21.980888934376658
31	22.476250732714906	27.342966402113937	28.096988192822653	22.083794672348503
32	21.86272411771821	27.163951357034527	28.204546088930655	22.76877843631661
33	22.03150441489807	27.230384175218887	28.5614596611368	22.17665174874624
34	22.496161969537667	26.821180345562308	28.2304120881677	22.452245596732322
35	22.29742177396094	27.582273416637975	28.309870949133302	21.81043386026778
36	22.6792709137769	27.220893772621118	28.072610884189174	22.027224429412804
37	22.704764740363053	27.002056253896185	27.953329549578047	22.339849456162714
38	22.73211943020367	27.139201875750164	27.81655610037496	22.312122593671205
39	22.44536040269081	27.24341021800013	27.927277464015553	22.383951915293505
40	22.091424211691805	27.48829982228756	28.3459716962699	22.074304269750737
41	22.32105473729263	26.74302408887483	28.262418936144478	22.673502237688066
42	22.178140439349814	27.22796505298808	27.9507243410218	22.643170166640306
43	22.418191799175638	26.87142365343283	28.172167068302983	22.538217479088548
>>END_MODULE
>>Per sequence GC content	pass
#GC Content	Count
0	18.0
1	24.5
2	31.0
3	119.5
4	208.0
5	208.0
6	285.5
7	363.0
8	368.0
9	373.0
10	505.0
11	637.0
12	637.0
13	989.0
14	1341.0
15	2523.0
16	3705.0
17	4178.0
18	4651.0
19	4651.0
20	4721.0
21	4791.0
22	5424.0
23	6057.0
24	7640.5
25	9224.0
26	9224.0
27	11594.5
28	13965.0
29	16647.5
30	19330.0
31	22134.5
32	24939.0
33	24939.0
34	27913.0
35	30887.0
36	32981.0
37	35075.0
38	36594.5
39	38114.0
40	38114.0
41	38905.5
42	39697.0
43	39662.5
44	39628.0
45	39417.0
46	39206.0
47	39206.0
48	38191.0
49	37176.0
50	37371.0
51	37566.0
52	36738.0
53	35910.0
54	35910.0
55	33268.5
56	30627.0
57	27655.5
58	24684.0
59	21609.0
60	18534.0
61	18534.0
62	16352.0
63	14170.0
64	11672.0
65	9174.0
66	7759.0
67	6344.0
68	6344.0
69	5175.5
70	4007.0
71	3488.0
72	2969.0
73	2411.0
74	1853.0
75	1853.0
76	1371.0
77	889.0
78	699.0
79	509.0
80	395.5
81	282.0
82	282.0
83	238.0
84	194.0
85	156.5
86	119.0
87	100.0
88	81.0
89	81.0
90	62.5
91	44.0
92	28.0
93	12.0
94	6.5
95	1.0
96	1.0
97	0.5
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
43	537385.0
>>END_MODULE
>>Sequence Duplication Levels	pass
#Total Deduplicated Percentage	70.86159069475951
#Duplication Level	Percentage of deduplicated	Percentage of total
1	90.62907340395058	64.22120304596062
2	5.379739377067665	7.624337795644988
3	1.3765815887619504	2.9264028330237326
4	0.6335128711749823	1.7956691910825402
5	0.3851195557543187	1.3645092164205075
6	0.2631000904054646	1.1186214550839755
7	0.20062812174460828	0.9951779491447368
8	0.13482155645190605	0.7642935960100304
9	0.11066499121388776	0.7057707580473904
>10	0.7901885571348537	10.935177516193267
>50	0.06367245567366603	3.1132326344886962
>100	0.03210152508557147	3.4160956364208945
>500	0.0	0.0
>1k	7.959055806338923E-4	1.0195083724786038
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT	2402	0.4469793537221918	No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT	1859	0.34593447900481034	No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT	1162	0.21623231016868727	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	3.7217265089274914E-4	0.0	0.0
2	0.0	1.8608632544637457E-4	3.7217265089274914E-4	0.0	0.0
3	0.0	1.8608632544637457E-4	5.582589763391238E-4	0.0	0.0
4	0.0	1.8608632544637457E-4	5.582589763391238E-4	1.8608632544637457E-4	0.0
5	0.0	1.8608632544637457E-4	5.582589763391238E-4	1.8608632544637457E-4	0.0
6	0.0	1.8608632544637457E-4	0.001302604278124622	1.8608632544637457E-4	0.0
7	0.0	1.8608632544637457E-4	0.0027912948816956186	3.7217265089274914E-4	0.0
8	0.0	1.8608632544637457E-4	0.0037217265089274916	3.7217265089274914E-4	0.0
9	0.0	1.8608632544637457E-4	0.00409389915982024	9.304316272318729E-4	0.0
10	0.0	1.8608632544637457E-4	0.004838244461605739	9.304316272318729E-4	0.0
11	0.0	1.8608632544637457E-4	0.007071280366962234	0.001302604278124622	0.0
12	0.0	1.8608632544637457E-4	0.007629539343301357	0.0014886906035709965	0.0
13	0.0	1.8608632544637457E-4	0.008373884645086855	0.0014886906035709965	0.0
14	0.0	1.8608632544637457E-4	0.008746057295979605	0.0014886906035709965	0.0
15	0.0	1.8608632544637457E-4	0.009676488923211478	0.0014886906035709965	0.0
16	0.0	1.8608632544637457E-4	0.010793006875889724	0.0014886906035709965	0.0
17	0.0	1.8608632544637457E-4	0.01135126585222885	0.001674776929017371	0.0
18	0.0	1.8608632544637457E-4	0.011537352177675224	0.001674776929017371	0.0
19	0.0	1.8608632544637457E-4	0.012095611154014347	0.001674776929017371	0.0
20	0.0	1.8608632544637457E-4	0.012281697479460722	0.001674776929017371	0.0
21	0.0	1.8608632544637457E-4	0.01265387013035347	0.0018608632544637458	0.0
22	0.0	1.8608632544637457E-4	0.01265387013035347	0.00204694957991012	0.0
23	0.0	1.8608632544637457E-4	0.01302604278124622	0.002605208556249244	0.0
24	0.0	1.8608632544637457E-4	0.01302604278124622	0.003535640183481117	0.0
25	0.0	1.8608632544637457E-4	0.01302604278124622	0.003535640183481117	0.0
26	0.0	1.8608632544637457E-4	0.01302604278124622	0.004652158136159364	0.0
27	0.0	1.8608632544637457E-4	0.01302604278124622	0.005582589763391237	0.0
28	0.0	1.8608632544637457E-4	0.01302604278124622	0.011537352177675224	0.0
29	1.8608632544637457E-4	1.8608632544637457E-4	0.01302604278124622	0.024563394958921445	0.0
30	1.8608632544637457E-4	1.8608632544637457E-4	0.013212129106692595	0.042241595876327025	0.0
31	1.8608632544637457E-4	1.8608632544637457E-4	0.013212129106692595	0.09750923453390027	0.0
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
TGCGAAT	40	1.5979676E-6	32.375	13
AACTGCG	60	4.3137334E-8	27.750002	10
GGTATCA	690	0.0	27.07971	1
ACTGCGA	60	1.3351928E-6	24.666668	11
GCGAATG	55	1.900215E-5	23.545454	14
CTGCGAA	70	5.0935832E-6	21.142859	12
CGCTACA	70	5.0935832E-6	21.142859	2
GTATCAA	1600	0.0	20.696877	1
CCGGCAG	145	0.0	20.413794	16
GCCGCTC	145	0.0	20.413794	27
TTCGCCG	145	0.0	20.413794	24
CCGCTCT	140	3.6379788E-12	19.82143	28
CGGCAGC	160	0.0	19.65625	17
CGAACTA	95	1.6719605E-7	19.473684	29
AGCTTCG	160	1.8189894E-12	18.5	21
CGTTAGA	50	0.0070301257	18.5	25
GCTTCGC	170	0.0	18.5	22
CTTTCCG	50	0.0070301257	18.5	21
GCCGGCA	165	3.6379788E-12	17.939394	15
CGCTCTC	155	2.0008883E-11	17.903225	29
>>END_MODULE