FastQCFastQC Report
Fri 10 Feb 2017
ERR1633291.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1633291.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1298626
Sequences flagged as poor quality0
Sequence length43
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT80950.6233511418992074No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT56760.4370773417442743No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT32020.2465682960298038No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT14050.10819127293000448No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA16950.025.9764021
GTATCAA45600.022.922151
ACGGCAT609.237696E-418.516
CGGGCTA500.007035363518.49999817
TTCGTGC953.607116E-617.52631612
CCGCTCT3500.016.91428628
TTCGCCG3550.016.67605824
GTCGCCA2450.016.61224412
TCCGCCG2350.016.53191631
GCCGCTC3500.016.38571527
GTCCGGC3200.016.187515
CGCCGTC2750.016.14545426
CGTCCGC2650.016.05660429
TGCTCGC3950.015.9240510
CGAGTCG2800.015.85714321
TCGCCAT2450.015.85714213
TAGTACC2100.015.8571424
CTGCTCG5550.015.6666679
TATCAAC66250.015.6377362
CGCTCTC3800.015.57894829
Produced by FastQC (version 0.11.5)