FastQCFastQC Report
Fri 10 Feb 2017
ERR1633290.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1633290.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences883433
Sequences flagged as poor quality0
Sequence length43
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT264502.9940018088525107No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT197432.234804450365789No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT110451.2502362940936098No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT46780.5295251592367503No Hit
GTATGAAACTGCTCGCCGGCAGCTTCGCCGCTCTCTTCCTGAG43810.49590631094831183No Hit
ATCATTAACTGAATCCATAGGTTAATGAGGCGAACCGGGGGAA41640.47134304469042926No Hit
GTACTGGTTCACTATCGGTCAGTCAGGAGTATTTAGCCTTGGA34470.39018239074157296No Hit
GTTAATGATAGTGTGTCGAAACACACTGGGTTTCCCCATTCGG32970.3732031744342808No Hit
TCTTTAGTGACCATGAAGTGCGATTGCCTCGGTGATAACGACA31920.3613177230191763No Hit
GTATGAAACTGCTTGTCCGGCGAGTCGCCGTCCGCCGCCTGGG28870.32679331652768234No Hit
ATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTT27200.3078897890388971No Hit
ATACAGGGTGACAGCCCCGTACACAAAAATGCACATGCTGTGA26370.2984946226821955No Hit
CCGTATTACCGCGGCTGCTGGCACGGAGTTAGCCGGTGCTTCT26220.29679670105146627No Hit
ACCATGAAGTGCGATTGCCTCGGTGATAACGACATTGGGGCCA26040.2947591950945912No Hit
TCTTTAGTGGCCCCAATGTCGTTATCACCGAGGCAATCGCACT23930.2708750974890003No Hit
GTTAATACCTTTGCTCATTGACGTTACCCGCAGAAGAAGCACC23850.2699695392859447No Hit
TATTCAGACAGGATACCACGTGTCCCGCCCTACTCATCGAGCT19920.22548399256083934No Hit
CATCTTTAGTGACCATGAAGTGCGATTGCCTCGGTGATAACGA19500.22072981199479755No Hit
TCATTAACCTATGGATTCAGTTAATGATAGTGTGTCGAAACAC18840.21325895681958904No Hit
GGTTAATGAGGCGAACCGGGGGAACTGAAACATCTAAGTACCC18510.20952352923198475No Hit
GTCATCTTTAGTGGCCCCAATGTCGTTATCACCGAGGCAATCG17830.20182628450601234No Hit
GGATACCACGTGTCCCGCCCTACTCATCGAGCTCACAGCATGT17730.2006943367521929No Hit
CCCCAATGTCGTTATCACCGAGGCAATCGCACTTCATGGTCAC16730.1893748592139981No Hit
GGTATTAACTTTACTCCCTTCCTCCCCGCTGAAAGTACTTTAC16680.18880888533708837No Hit
CCGTCATCTTTAGTGACCATGAAGTGCGATTGCCTCGGTGATA16550.18733735325712306No Hit
GAGTTAGCCGGTGCTTCTTCTGCGGGTAACGTCAATGAGCAAA16350.1850734577494841No Hit
GTATTTAGCCTTGGAGGATGGTCCCCCCATATTCAGACAGGAT16000.18111164061111595No Hit
CTATCGGTCAGTCAGGAGTATTTAGCCTTGGAGGATGGTCCCC15930.18031927718344234No Hit
GCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT15900.17997969285729648No Hit
GTATCAACGCAGAGTACGGGGATTTCACATCTGACTTAACAAA15840.17930052420500478No Hit
GTGGATGCCCTGGCAGTCAGAGGCGATGAAGGACGTGCTAATC15830.17918732942962284No Hit
GGTGATATGAACCGTTATAACCGGCGATTTCCGAATGGGGAAA15810.17896093987885894No Hit
CCCATATTCAGACAGGATACCACGTGTCCCGCCCTACTCATCG15620.17681023914660193No Hit
GTAATACGGAGGGTGCAAGCGTTAATCGGAATTACTGGGCGTA14960.16933938397139342No Hit
AGCGTACACGGTGGATGCCCTGGCAGTCAGAGGCGATGAAGGA14790.1674150727899003No Hit
ACTTAGATGTTTCAGTTCCCCCGGTTCGCCTCATTAACCTATG14700.16639631981146277No Hit
CATCTTTAGTGGCCCCAATGTCGTTATCACCGAGGCAATCGCA14310.16198172357156682No Hit
ACCCTGTATCGCGCGCCTTTCCAGACGCTTCCACTAACACACA14300.16186852879618488No Hit
TAATGATAGTGTGTCGAAACACACTGGGTTTCCCCATTCGGAA14110.1597178280639279No Hit
CCGTCATCTTTAGTGGCCCCAATGTCGTTATCACCGAGGCAAT14010.1585858803101084No Hit
GTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT13970.15813310120858062No Hit
CCTATGGATTCAGTTAATGATAGTGTGTCGAAACACACTGGGT13720.15530323182403194No Hit
TCACGGTACTGGTTCACTATCGGTCAGTCAGGAGTATTTAGCC13680.15485045272250414No Hit
GTTATAACCGGCGATTTCCGAATGGGGAAACCCAGTGTGTTTC13680.15485045272250414No Hit
CGTTATCACCGAGGCAATCGCACTTCATGGTCACTAAAGATGA13650.1545108683963583No Hit
GTATTACCGCGGCTGCTGGCACGGAGTTAGCCGGTGCTTCTTC13500.1528129467656291No Hit
GATATGAACCGTTATAACCGGCGATTTCCGAATGGGGAAACCC13470.15247336243948323No Hit
TATCAACGCAGAGTACGGGGATTTCACATCTGACTTAACAAAC13210.1495302982795526No Hit
ATGATAGTGTGTCGAAACACACTGGGTTTCCCCATTCGGAAAT12970.14681362367038586No Hit
GTTCACTATCGGTCAGTCAGGAGTATTTAGCCTTGGAGGATGG12690.14364416995969134No Hit
GAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT12550.14205944310434407No Hit
GTCAGGAGTATTTAGCCTTGGAGGATGGTCCCCCCATATTCAG12440.14081430057514266No Hit
GTGCTAATCTGCGATAAGCGTCGGTAAGGTGATATGAACCGTT12420.14058791102437876No Hit
CGGTACTGGTTCACTATCGGTCAGTCAGGAGTATTTAGCCTTG12270.13888998939364955No Hit
GTCATCTTTAGTGACCATGAAGTGCGATTGCCTCGGTGATAAC12270.13888998939364955No Hit
GTATCAACGCAGAGTACGGGCTCCGTCATCTTTAGTGACCATG12260.1387767946182676No Hit
GTCGTTATCACCGAGGCAATCGCACTTCATGGTCACTAAAGAT12260.1387767946182676No Hit
GAATATGGGGGGACCATCCTCCAAGGCTAAATACTCCTGACTG12240.13855040506750368No Hit
GTGGTATCCTGTCTGAATATGGGGGGACCATCCTCCAAGGCTA11880.1344753931537536No Hit
TCTAAGTACCCCGAGGAAAAGAAATCAACCGAGATTCCCCCAG11520.13040038124000347No Hit
GTATCAACGCAGAGTACGGGACTAGTATGAAACTGCTTGTCCG11490.13006079691385766No Hit
CGCTTGCACCCTCCGTATTACCGCGGCTGCTGGCACGGAGTTA11410.12915523871080206No Hit
ATCTTTAGTGGCCCCAATGTCGTTATCACCGAGGCAATCGCAC11330.1282496805077465No Hit
CTTTACGCCCAGTAATTCCGATTAACGCTTGCACCCTCCGTAT11160.12632536932625338No Hit
GTCCAGACTCCTACGGGAGGCAGCAGTGGGGAATATTGCACAA11130.12598578500010751No Hit
ATATTGCACAATGGGCGCAAGCCTGATGCAGCCATGCCGCGTG11120.12587259022472558No Hit
GTCTGAATATGGGGGGACCATCCTCCAAGGCTAAATACTCCTG10850.12281633128941301No Hit
CCGTTATAACCGGCGATTTCCGAATGGGGAAACCCAGTGTGTT10760.12179757831097549No Hit
ATCCATAGGTTAATGAGGCGAACCGGGGGAACTGAAACATCTA10710.12123160443406573No Hit
GTGTGTGTGTTAGTGGAAGCGTCTGGAAAGGCGCGCGATACAG10660.120665630557156No Hit
TTAGTGGCCCCAATGTCGTTATCACCGAGGCAATCGCACTTCA10260.1161378395418781No Hit
GGCTAACTCCGTGCCAGCAGCCGCGGTAATACGGAGGGTGCAA10130.1146663074619128No Hit
GTAAAGTACTTTCAGCGGGGAGGAAGGGAGTAAAGTTAATACC10040.11364755448347527No Hit
GGTTCACTATCGGTCAGTCAGGAGTATTTAGCCTTGGAGGATG10020.11342116493271137No Hit
GCCTGAATCAGTGTGTGTGTTAGTGGAAGCGTCTGGAAAGGCG10000.11319477538194746No Hit
AGTTAATACCTTTGCTCATTGACGTTACCCGCAGAAGAAGCAC9890.11194963285274605No Hit
GCCTTGGAGGATGGTCCCCCCATATTCAGACAGGATACCACGT9790.11081768509892659No Hit
TCGGTAAGGTGATATGAACCGTTATAACCGGCGATTTCCGAAT9580.10844059481590568No Hit
CAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT9560.10821420526514179No Hit
GCCCAGAGCCTGAATCAGTGTGTGTGTTAGTGGAAGCGTCTGG9550.10810101048975983No Hit
GGGTTTCCCCATTCGGAAATCGCCGGTTATAACGGTTCATATC9550.10810101048975983No Hit
CTCCGTATTACCGCGGCTGCTGGCACGGAGTTAGCCGGTGCTT9470.10719545228670425No Hit
GTATGAAGAAGGCCTTCGGGTTGTAAAGTACTTTCAGCGGGGA9390.10628989408364867No Hit
CAGTAATTCCGATTAACGCTTGCACCCTCCGTATTACCGCGGC9150.10357321947448193No Hit
TCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTT8960.10142251874222492No Hit
TGTATGAAGAAGGCCTTCGGGTTGTAAAGTACTTTCAGCGGGG8940.10119612919146104No Hit
CATTAACCTATGGATTCAGTTAATGATAGTGTGTCGAAACACA8920.10096973964069714No Hit
GGAGTAAAGTTAATACCTTTGCTCATTGACGTTACCCGCAGAA8880.10051696053916936No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTGAAT251.2333944E-437.018
GTTGCGC251.2333944E-437.09
CTCGTGA303.5988103E-430.83333216
CCTCGTG303.5988103E-430.83333215
CGCTCGT303.5988103E-430.83333213
GCTCGTT303.5988103E-430.83333214
TTGGCTG250.005495663329.635
TAGCAAC250.005495663329.61
GCAGCTC250.005495663329.619
GGTTGCG405.9385988E-527.758
CTCACTC358.867765E-426.4285727
AGTCTCT358.867765E-426.4285722
ATACGGG358.867765E-426.428573
CTCTCAC358.867765E-426.4285725
GTGAATT358.867765E-426.4285719
TACCCTA509.0879785E-625.90000212
TGTCAAC659.386713E-825.6153852
GATAAGG451.3228733E-424.6666682
CTCCACT1051.8189894E-1224.6666682
CGAGGGA451.3228733E-424.66666816
Produced by FastQC (version 0.11.5)