FastQCFastQC Report
Fri 10 Feb 2017
ERR1633289.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1633289.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences854766
Sequences flagged as poor quality0
Sequence length43
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT63200.7393836441786407No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT46700.5463483573282044No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT27520.32195946024993977No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT11480.13430576321472779No Hit
ATCATTAACTGAATCCATAGGTTAATGAGGCGAACCGGGGGAA10130.11851196701787389No Hit
GTATGAAACTGCTCGCCGGCAGCTTCGCCGCTCTCTTCCTGAG9230.10798276955330466No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA13850.025.7797831
CTACGAC400.001930784723.1253
GCCGGCA2700.021.24074215
GTATAAC705.0994004E-621.1428571
GTATCAA32100.020.7476621
AGCTTCG2950.020.69491821
TAGGACC1600.019.656254
TTCGCCG2900.019.1379324
CCGGCAG3000.018.516
AGGACCG801.6158807E-518.55
AACGGCC1306.9485395E-1018.537
TTAACGG1205.167749E-918.535
TGCTCGC3400.018.510
GGACCGT1205.167749E-918.56
TAACGGC1258.569259E-917.7636
TATAGAG1054.79371E-717.6190475
CGCTCTC3050.017.59016229
CGAACTA1502.5102054E-1017.26666629
TGCCGAG752.0664852E-417.26666611
TCACGTA1401.8662831E-917.17857225
Produced by FastQC (version 0.11.5)