##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	ERR1633289.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	854766
Sequences flagged as poor quality	0
Sequence length	43
%GC	46
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.254928249368835	33.0	33.0	33.0	27.0	33.0
2	31.52464417162124	33.0	33.0	33.0	27.0	33.0
3	31.27351111298297	33.0	33.0	33.0	27.0	33.0
4	31.589005645989662	33.0	33.0	33.0	27.0	33.0
5	31.697069139390194	33.0	33.0	33.0	27.0	33.0
6	34.58879740186203	37.0	37.0	37.0	27.0	37.0
7	34.783084493299924	37.0	37.0	37.0	27.0	37.0
8	34.9032799620013	37.0	37.0	37.0	27.0	37.0
9	35.05692903086927	37.0	37.0	37.0	27.0	37.0
10	35.04570139663955	37.0	37.0	37.0	27.0	37.0
11	35.09208953093595	37.0	37.0	37.0	33.0	37.0
12	35.07804475142905	37.0	37.0	37.0	33.0	37.0
13	35.06004801314044	37.0	37.0	37.0	33.0	37.0
14	35.08018451833601	37.0	37.0	37.0	33.0	37.0
15	35.08071214812007	37.0	37.0	37.0	33.0	37.0
16	35.070681332668826	37.0	37.0	37.0	33.0	37.0
17	35.062789114213714	37.0	37.0	37.0	33.0	37.0
18	35.03476624011718	37.0	37.0	37.0	27.0	37.0
19	35.00680771111626	37.0	37.0	37.0	27.0	37.0
20	35.00914519295339	37.0	37.0	37.0	27.0	37.0
21	35.01036307012679	37.0	37.0	37.0	27.0	37.0
22	34.903671882129146	37.0	37.0	37.0	27.0	37.0
23	34.95041800913934	37.0	37.0	37.0	27.0	37.0
24	34.948305150181454	37.0	37.0	37.0	27.0	37.0
25	34.965446683653774	37.0	37.0	37.0	27.0	37.0
26	34.8400497914049	37.0	37.0	37.0	27.0	37.0
27	34.855044538505275	37.0	37.0	37.0	27.0	37.0
28	34.861097657136575	37.0	37.0	37.0	27.0	37.0
29	34.858149481846496	37.0	37.0	37.0	27.0	37.0
30	34.86251792888346	37.0	37.0	37.0	27.0	37.0
31	34.84332320190555	37.0	37.0	37.0	27.0	37.0
32	34.8252293610181	37.0	37.0	37.0	27.0	37.0
33	34.81330562984489	37.0	37.0	37.0	27.0	37.0
34	34.774354618690964	37.0	37.0	37.0	27.0	37.0
35	34.69888601090825	37.0	37.0	37.0	27.0	37.0
36	34.71811466530021	37.0	37.0	37.0	27.0	37.0
37	34.7071619601154	37.0	37.0	37.0	27.0	37.0
38	34.67835056611985	37.0	37.0	37.0	27.0	37.0
39	34.573067950760795	37.0	37.0	37.0	27.0	37.0
40	34.330945545330536	37.0	37.0	37.0	27.0	37.0
41	34.505277467751405	37.0	37.0	37.0	27.0	37.0
42	34.53113483690273	37.0	37.0	37.0	27.0	37.0
43	34.11688228123253	37.0	37.0	37.0	27.0	37.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
14	6.0
15	35.0
16	53.0
17	43.0
18	28.0
19	58.0
20	134.0
21	401.0
22	913.0
23	2066.0
24	3769.0
25	6242.0
26	9439.0
27	13387.0
28	18042.0
29	23278.0
30	29371.0
31	36111.0
32	45048.0
33	58606.0
34	83775.0
35	158580.0
36	365381.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	42.01430566962186	19.9972858068758	13.88134296403928	24.10706555946306
2	16.51843896458212	22.4006336237052	37.69207011041618	23.388857301296497
3	18.483070220387802	27.4409604499945	29.898475138224967	24.177494191392732
4	12.911486886469515	18.254235662157832	38.666839813469416	30.167437637903237
5	14.711511688579096	36.52906175491304	34.122555178844266	14.636871377663594
6	29.34171457451513	39.732979552298524	16.80588605536486	14.119419817821486
7	26.615237386606395	32.52656282538145	22.648420737371396	18.20977905064076
8	25.362145897239714	34.71312616552366	20.036009855328825	19.888718081907797
9	27.00668955012249	14.708703902588544	19.715688270240044	38.56891827704892
10	15.619362492190843	27.92518654228175	32.62506931721664	23.830381648310766
11	34.56934412459082	23.461040799470265	23.181081137995662	18.78853393794325
12	23.621786547429355	25.826483505427216	29.566571435925155	20.985158511218273
13	29.384416319788105	22.278962897447958	25.351382717609265	22.98523806515467
14	22.664214533568252	20.974161349421948	28.05387673351537	28.30774738349443
15	24.659731435270004	27.950573607279654	24.774967651965568	22.614727305484774
16	22.704108492850676	27.864936134567824	25.24000720665071	24.19094816593079
17	22.85338911468168	26.684847080955485	26.079769199991574	24.381994604371254
18	24.306184382626356	25.58864063381089	27.57889293678036	22.526282046782395
19	25.713704101473382	25.447666378868604	27.58813523233259	21.25049428732542
20	25.08955667399031	25.20151714036356	27.54882622846487	22.160099957181263
21	23.546561281099155	26.349667628333368	27.58158373168797	22.522187358879506
22	23.323810259182046	26.020337729858227	27.718697280893252	22.937154730066474
23	22.962892768313196	25.91130204055847	28.225736634353733	22.900068556774603
24	23.272217191605655	26.2090443466399	27.54473154056198	22.974006921192466
25	23.209275988984118	25.92838273866766	28.384727516068725	22.477613756279496
26	23.526321823750592	26.1004766216719	27.75765531151216	22.615546243065353
27	23.458934959977352	26.017178970618858	27.680090223523163	22.843795845880628
28	22.857366811501628	25.979156868663473	27.856980740927927	23.306495578906976
29	23.728833388319142	25.95224891958735	27.406331089444365	22.912586602649146
30	23.53802093204456	25.915747701710174	27.8934819588051	22.652749407440165
31	23.487948748546387	25.807062985659236	27.78468025167122	22.920308014123165
32	22.439357672158227	25.888020815053476	27.95490227734842	23.717719235439873
33	22.41490653582384	26.010393487808358	28.52277699393752	23.051922982430277
34	23.109950559568347	25.653804667008277	28.018428435384656	23.217816338038716
35	22.891645198802948	26.30123331999635	27.758591240175672	23.048530241025027
36	23.48069530140413	26.040811169372667	27.6344637011767	22.844029828046505
37	23.498594937093895	25.981496690322263	27.409723830849615	23.11018454173423
38	23.273738075683873	26.210565230718114	27.315546009083185	23.200150684514824
39	23.01354990722607	25.801564404761066	27.715538521653883	23.469347166358983
40	23.06116527798251	26.38511592646409	27.840017033901677	22.713701761651727
41	23.26449578013164	25.74271789004242	27.671081910136806	23.321704419689134
42	22.697790974371934	26.5308868158069	27.625455387790343	23.145866822030825
43	23.111237461480684	26.128554481577414	27.435578860179277	23.324629196762622
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	22.0
1	45.5
2	69.0
3	289.5
4	510.0
5	510.0
6	691.5
7	873.0
8	885.0
9	897.0
10	1213.5
11	1530.0
12	1530.0
13	2429.5
14	3329.0
15	6008.5
16	8688.0
17	9468.5
18	10249.0
19	10249.0
20	9016.0
21	7783.0
22	7420.5
23	7058.0
24	8659.0
25	10260.0
26	10260.0
27	13053.0
28	15846.0
29	18746.5
30	21647.0
31	24869.0
32	28091.0
33	28091.0
34	33384.0
35	38677.0
36	41149.5
37	43622.0
38	46945.0
39	50268.0
40	50268.0
41	53124.0
42	55980.0
43	58418.5
44	60857.0
45	62561.5
46	64266.0
47	64266.0
48	65398.5
49	66531.0
50	68396.5
51	70262.0
52	68399.5
53	66537.0
54	66537.0
55	62195.5
56	57854.0
57	51918.5
58	45983.0
59	41969.5
60	37956.0
61	37956.0
62	32849.0
63	27742.0
64	23022.5
65	18303.0
66	15534.0
67	12765.0
68	12765.0
69	10328.0
70	7891.0
71	6666.5
72	5442.0
73	4362.0
74	3282.0
75	3282.0
76	2433.5
77	1585.0
78	1219.5
79	854.0
80	674.5
81	495.0
82	495.0
83	407.0
84	319.0
85	263.5
86	208.0
87	183.0
88	158.0
89	158.0
90	107.0
91	56.0
92	37.0
93	18.0
94	10.5
95	3.0
96	3.0
97	1.5
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
43	854766.0
>>END_MODULE
>>Sequence Duplication Levels	warn
#Total Deduplicated Percentage	59.48168101543544
#Duplication Level	Percentage of deduplicated	Percentage of total
1	85.58480083216968	50.907278228686906
2	8.368801676644889	9.955807836232651
3	2.194424470144347	3.9158416903677593
4	0.9954437252129531	2.368426645277346
5	0.59751461730845	1.7770586934400603
6	0.400840302737227	1.4305593015327787
7	0.2936822048130994	1.2228097858621791
8	0.20257792795714513	0.9639740553211814
9	0.15265936333774877	0.817239199866789
>10	1.0297297988615248	11.613205346244047
>50	0.10919058087079646	4.5130609864894975
>100	0.06714653394301585	7.765759949166384
>500	0.0021917266244330276	0.8642185655869321
>1k	7.969914997938282E-4	1.1357386881540825
>5k	1.9924787494845705E-4	0.7490210277714496
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT	6320	0.7393836441786407	No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT	4670	0.5463483573282044	No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT	2752	0.32195946024993977	No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	1148	0.13430576321472779	No Hit
ATCATTAACTGAATCCATAGGTTAATGAGGCGAACCGGGGGAA	1013	0.11851196701787389	No Hit
GTATGAAACTGCTCGCCGGCAGCTTCGCCGCTCTCTTCCTGAG	923	0.10798276955330466	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	3.50973248818975E-4	0.0	0.0
2	0.0	0.0	4.6796433175863334E-4	0.0	0.0
3	0.0	0.0	7.0194649763795E-4	0.0	0.0
4	0.0	0.0	9.359286635172667E-4	1.1699108293965833E-4	0.0
5	0.0	0.0	0.0011699108293965834	1.1699108293965833E-4	0.0
6	0.0	0.0	0.0028077859905518	1.1699108293965833E-4	0.0
7	0.0	0.0	0.006551500644620867	4.6796433175863334E-4	0.0
8	0.0	0.0	0.007838402556957108	4.6796433175863334E-4	0.0
9	0.0	0.0	0.009476277718112325	8.189375805776084E-4	0.0
10	8.189375805776084E-4	0.0	0.010880170713388225	9.359286635172667E-4	0.0
11	8.189375805776084E-4	0.0	0.017080698109190117	0.001052919746456925	0.0
12	8.189375805776084E-4	0.0	0.019537510850922944	0.001052919746456925	0.0
13	8.189375805776084E-4	0.0	0.020941403846198844	0.001052919746456925	0.0
14	8.189375805776084E-4	0.0	0.0224622879244144	0.001052919746456925	0.0
15	8.189375805776084E-4	0.0	0.02456812741732825	0.001052919746456925	0.0
16	8.189375805776084E-4	0.0	0.025621047163785177	0.001052919746456925	0.0
17	8.189375805776084E-4	0.0	0.02620600257848347	0.001052919746456925	0.0
18	8.189375805776084E-4	0.0	0.0266739669102421	0.0011699108293965834	0.0
19	8.189375805776084E-4	0.0	0.027726886656699028	0.0012869019123362417	0.0
20	8.189375805776084E-4	0.0	0.02831184207139732	0.0014038929952759	0.0
21	8.189375805776084E-4	0.0	0.02959874398373356	0.0016378751611552168	0.0
22	9.359286635172667E-4	0.0	0.029832726149612878	0.001988848409974192	0.0
23	9.359286635172667E-4	0.0	0.029949717232552533	0.002456812741732825	0.0
24	9.359286635172667E-4	0.0	0.03030069048137151	0.0032757503223104336	0.0
25	9.359286635172667E-4	0.0	0.030534672647250828	0.0036267235711294086	0.0
26	9.359286635172667E-4	0.0	0.030534672647250828	0.00491362548346565	0.0
27	9.359286635172667E-4	0.0	0.030534672647250828	0.007253447142258817	0.0
28	0.001052919746456925	0.0	0.030651663730190484	0.0168467159433108	0.0
29	0.001052919746456925	0.0	0.030651663730190484	0.04293572743885461	0.0
30	0.001052919746456925	0.0	0.030651663730190484	0.07931995423308835	0.0
31	0.001052919746456925	0.0	0.030651663730190484	0.15173743457273686	0.0
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GGTATCA	1385	0.0	25.779783	1
CTACGAC	40	0.0019307847	23.125	3
GCCGGCA	270	0.0	21.240742	15
GTATAAC	70	5.0994004E-6	21.142857	1
GTATCAA	3210	0.0	20.747662	1
AGCTTCG	295	0.0	20.694918	21
TAGGACC	160	0.0	19.65625	4
TTCGCCG	290	0.0	19.13793	24
CCGGCAG	300	0.0	18.5	16
AGGACCG	80	1.6158807E-5	18.5	5
AACGGCC	130	6.9485395E-10	18.5	37
TTAACGG	120	5.167749E-9	18.5	35
TGCTCGC	340	0.0	18.5	10
GGACCGT	120	5.167749E-9	18.5	6
TAACGGC	125	8.569259E-9	17.76	36
TATAGAG	105	4.79371E-7	17.619047	5
CGCTCTC	305	0.0	17.590162	29
CGAACTA	150	2.5102054E-10	17.266666	29
TGCCGAG	75	2.0664852E-4	17.266666	11
TCACGTA	140	1.8662831E-9	17.178572	25
>>END_MODULE