FastQCFastQC Report
Fri 10 Feb 2017
ERR1633288.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1633288.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences978568
Sequences flagged as poor quality0
Sequence length43
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT46670.47692137899461257No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT32920.3364099377866433No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT19590.2001904824192085No Hit
GTATTAGAGGCACCGCCTGCCCAGTGACACATGTTTAACGGCC12940.13223403994408156No Hit
TTATTACCCTCACTGTCAACCCAACACAGGCATGCTCATAAGG11860.12119750492556469No Hit
GTACATGCTAAGACTTCACCAGTCAAAGCGAACTACTATACTC11660.11915370214435787No Hit
CCCCTATACCTTCTGCATAATGAATTAACTAGAAATAACTTTG11480.11731427964127172No Hit
AGCATGTACTGCTCGGAGGTTGGGTTCTGCTCCGAGGTCGCCC11210.11455514588664252No Hit
GTATGAATGGCTCCACGAGGGTTCAGCTGTCTCTTACTTTTAA11060.1130222938007374No Hit
CTCTAATACTGGTGATGCTAGAGGTGATGTTTTTGGTAAACAG10160.1038251812853067No Hit
TGCTAAGACTTCACCAGTCAAAGCGAACTACTATACTCAATTG9880.10096385739161713No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA11700.024.3504261
GTATCAA27800.020.8956831
GCCGGCA1800.020.55555515
TAATTCG450.003825407720.5555555
TAGGACC3150.019.9682544
GGACCGT759.263464E-619.7333346
AGGACCG701.2189624E-418.55
CGCCGTT609.23548E-418.525
TGCGGTA1351.1514203E-917.81481436
GGACCCT1156.401933E-817.695656
GTTTAGG2350.017.3191491
CGGAATA752.066871E-417.26666627
ATTTCGG2150.017.20930329
CCGGCAG2050.017.14634316
GCCGCTC1851.8189894E-1217.027
TGGGGCG2100.016.73809437
TTGCGGT1554.0017767E-1016.70967935
AGCTTCG2050.016.24390421
CGGTTGG2400.016.187533
GTCTTAT700.002592124715.8571421
Produced by FastQC (version 0.11.5)