##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	ERR1633288.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	978568
Sequences flagged as poor quality	0
Sequence length	43
%GC	45
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.57058988235871	33.0	33.0	33.0	27.0	33.0
2	31.761386025294104	33.0	33.0	33.0	27.0	33.0
3	31.489052370402465	33.0	33.0	33.0	27.0	33.0
4	31.795143515831295	33.0	33.0	33.0	27.0	33.0
5	31.90485484912648	33.0	33.0	33.0	33.0	33.0
6	34.98366286246842	37.0	37.0	37.0	27.0	37.0
7	35.15443995716189	37.0	37.0	37.0	33.0	37.0
8	35.27621177066898	37.0	37.0	37.0	33.0	37.0
9	35.40916829489622	37.0	37.0	37.0	33.0	37.0
10	35.40293060880797	37.0	37.0	37.0	33.0	37.0
11	35.44152169292272	37.0	37.0	37.0	33.0	37.0
12	35.41737722876693	37.0	37.0	37.0	33.0	37.0
13	35.42068614546971	37.0	37.0	37.0	33.0	37.0
14	35.40608419649937	37.0	37.0	37.0	33.0	37.0
15	35.42547273158329	37.0	37.0	37.0	33.0	37.0
16	35.40716025866368	37.0	37.0	37.0	33.0	37.0
17	35.3875387300627	37.0	37.0	37.0	33.0	37.0
18	35.38244352972916	37.0	37.0	37.0	33.0	37.0
19	35.36469719017994	37.0	37.0	37.0	33.0	37.0
20	35.35766548671119	37.0	37.0	37.0	33.0	37.0
21	35.36487091341634	37.0	37.0	37.0	33.0	37.0
22	35.25708790804523	37.0	37.0	37.0	33.0	37.0
23	35.31530665216929	37.0	37.0	37.0	33.0	37.0
24	35.2969594346024	37.0	37.0	37.0	33.0	37.0
25	35.32343383392876	37.0	37.0	37.0	33.0	37.0
26	35.20547269070724	37.0	37.0	37.0	33.0	37.0
27	35.2137633766892	37.0	37.0	37.0	33.0	37.0
28	35.23812244013702	37.0	37.0	37.0	33.0	37.0
29	35.24235311189411	37.0	37.0	37.0	33.0	37.0
30	35.2367326542458	37.0	37.0	37.0	33.0	37.0
31	35.22757130827904	37.0	37.0	37.0	33.0	37.0
32	35.21995814291904	37.0	37.0	37.0	33.0	37.0
33	35.21294585557672	37.0	37.0	37.0	33.0	37.0
34	35.19554594059892	37.0	37.0	37.0	33.0	37.0
35	35.12495197063464	37.0	37.0	37.0	33.0	37.0
36	35.15623543790518	37.0	37.0	37.0	33.0	37.0
37	35.14040107585778	37.0	37.0	37.0	33.0	37.0
38	35.12759154192657	37.0	37.0	37.0	33.0	37.0
39	35.03384537405679	37.0	37.0	37.0	27.0	37.0
40	34.80621070789153	37.0	37.0	37.0	27.0	37.0
41	34.98914945103457	37.0	37.0	37.0	27.0	37.0
42	35.02833834746282	37.0	37.0	37.0	27.0	37.0
43	34.6501214018852	37.0	37.0	37.0	27.0	37.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
14	4.0
15	35.0
16	43.0
17	51.0
18	39.0
19	49.0
20	100.0
21	267.0
22	693.0
23	1592.0
24	2920.0
25	5156.0
26	8017.0
27	11712.0
28	16148.0
29	21597.0
30	27756.0
31	35042.0
32	44050.0
33	57924.0
34	83121.0
35	164300.0
36	497952.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	41.4058093050253	20.49249515618741	13.729347372895905	24.372348165891385
2	16.235867103767955	21.644791164231815	38.8754792717522	23.243862460248035
3	17.77648564024166	27.6122865247995	30.0011854056131	24.61004242934574
4	12.320656305949102	18.12444306374212	39.91342451418808	29.641476116120696
5	13.78688042118688	36.96544338257535	35.66241691941695	13.585259276820825
6	28.91674364990476	40.49161632099149	17.236410755307755	13.355229273795995
7	26.590998275030454	32.29341241487562	23.571994996770794	17.54359431332314
8	24.413735172210824	35.71228570727839	20.6584519420214	19.215527178489385
9	26.17978515545164	15.365309309112906	20.440582565544755	38.0143229698907
10	15.19209702340563	27.767206775614977	33.96074672378414	23.07994947719525
11	33.64038063782997	23.71874003646144	24.04390905895145	18.59697026675714
12	23.034270485035275	26.1136681354796	29.942017315097164	20.91004406438796
13	27.79459373288315	22.408764643846926	26.21371228161967	23.58292934165025
14	22.106077451950195	21.183913637069676	28.12742701580268	28.582581895177444
15	24.282727413935465	28.33722337129356	24.94635017699332	22.43369903777765
16	22.782780552807775	28.52249409340996	25.29308131882506	23.401644034957204
17	22.351538165973135	27.452461147309133	26.444559805756985	23.75144088096075
18	23.037336189207085	27.05667873872843	28.032185806198445	21.87379926586604
19	24.908948586097235	26.074529312219486	27.82300259154193	21.19351951014135
20	24.345267779040395	26.0366167706281	27.852535541730365	21.76557990860114
21	22.875160438518325	26.920254903082874	27.88360134400471	22.320983314394095
22	22.7469118139976	26.86313061534814	27.60707482770742	22.782882742946835
23	22.47518823423615	26.79169970814496	28.305033477489555	22.42807858012933
24	22.83540847442385	27.33116145224451	26.92332060725468	22.91010946607696
25	22.985423598564434	26.863334995626264	27.92968909672092	22.221552309088384
26	22.854211460010955	27.12933592760033	27.86633121050351	22.150121401885205
27	22.954255606151026	27.01028441559503	27.04380278120683	22.991657197047115
28	22.396604017298746	26.76707188463142	28.12722263552456	22.70910146254527
29	23.986989151494836	26.60438518324736	27.12780307551442	22.28082258974338
30	23.25959974166333	26.44180067200235	27.900769287366845	22.397830298967474
31	22.95364246531667	26.70146581535468	27.727659191798626	22.617232527530025
32	21.960865264345454	26.49146507958568	28.088799143237875	23.45887051283099
33	21.94022285625526	26.57117338805275	28.664129626147595	22.824474129544395
34	23.0532778509005	26.281259963538556	28.140405163463345	22.525057022097595
35	22.78441559503274	27.232343587773155	27.648359643887805	22.3348811733063
36	23.525805053915516	26.738152075277345	27.48863645653649	22.24740641427065
37	23.0241536612683	26.302106751906866	27.454402759951275	23.219336826873555
38	23.04183255532574	26.72149508261051	27.29294234023594	22.94373002182781
39	22.49021018467802	26.58067707098536	27.709878107602126	23.21923463673449
40	22.51136354346351	27.48158533694133	27.883396963726586	22.123654155868575
41	22.54988922588926	26.259289083640585	27.980886356390155	23.209935334080004
42	22.33069137760483	27.14793453290931	27.640388813041096	22.880985276444765
43	23.04111722435232	26.075551213610094	27.667162629474905	23.216168932562685
>>END_MODULE
>>Per sequence GC content	pass
#GC Content	Count
0	25.0
1	42.0
2	59.0
3	249.0
4	439.0
5	439.0
6	551.5
7	664.0
8	654.5
9	645.0
10	842.0
11	1039.0
12	1039.0
13	1665.5
14	2292.0
15	4246.0
16	6200.0
17	6980.5
18	7761.0
19	7761.0
20	7255.0
21	6749.0
22	7174.0
23	7599.0
24	9988.0
25	12377.0
26	12377.0
27	16047.5
28	19718.0
29	23609.0
30	27500.0
31	33189.0
32	38878.0
33	38878.0
34	46556.5
35	54235.0
36	58811.0
37	63387.0
38	66953.0
39	70519.0
40	70519.0
41	72947.5
42	75376.0
43	76684.5
44	77993.0
45	77019.5
46	76046.0
47	76046.0
48	73338.5
49	70631.0
50	72076.5
51	73522.0
52	70455.5
53	67389.0
54	67389.0
55	63765.0
56	60141.0
57	52180.0
58	44219.0
59	39808.5
60	35398.0
61	35398.0
62	31156.0
63	26914.0
64	22348.0
65	17782.0
66	15019.0
67	12256.0
68	12256.0
69	10025.5
70	7795.0
71	6612.5
72	5430.0
73	4441.0
74	3452.0
75	3452.0
76	2562.0
77	1672.0
78	1327.0
79	982.0
80	784.0
81	586.0
82	586.0
83	475.5
84	365.0
85	321.0
86	277.0
87	221.0
88	165.0
89	165.0
90	113.5
91	62.0
92	41.5
93	21.0
94	14.5
95	8.0
96	8.0
97	4.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
43	978568.0
>>END_MODULE
>>Sequence Duplication Levels	warn
#Total Deduplicated Percentage	54.367713929136364
#Duplication Level	Percentage of deduplicated	Percentage of total
1	86.32903956100522	46.93512526629827
2	7.828740729631978	8.512614728280193
3	2.037826645166281	3.323759282447161
4	0.9230856364815379	2.00744223265292
5	0.5421397894802268	1.4737450492031587
6	0.34157775893520176	1.1142481129406732
7	0.25784850841614204	0.9813043759836324
8	0.1843451515578087	0.8017939571294583
9	0.15322731099945275	0.7497556749494151
>10	1.1323593455880971	12.328200205456977
>50	0.14871230648427355	5.642886431543519
>100	0.11119622275344491	11.34501403319735
>500	0.007996988596479704	2.925434749900798
>1k	0.0019040449039237392	1.85867590001659
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT	4667	0.47692137899461257	No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT	3292	0.3364099377866433	No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT	1959	0.2001904824192085	No Hit
GTATTAGAGGCACCGCCTGCCCAGTGACACATGTTTAACGGCC	1294	0.13223403994408156	No Hit
TTATTACCCTCACTGTCAACCCAACACAGGCATGCTCATAAGG	1186	0.12119750492556469	No Hit
GTACATGCTAAGACTTCACCAGTCAAAGCGAACTACTATACTC	1166	0.11915370214435787	No Hit
CCCCTATACCTTCTGCATAATGAATTAACTAGAAATAACTTTG	1148	0.11731427964127172	No Hit
AGCATGTACTGCTCGGAGGTTGGGTTCTGCTCCGAGGTCGCCC	1121	0.11455514588664252	No Hit
GTATGAATGGCTCCACGAGGGTTCAGCTGTCTCTTACTTTTAA	1106	0.1130222938007374	No Hit
CTCTAATACTGGTGATGCTAGAGGTGATGTTTTTGGTAAACAG	1016	0.1038251812853067	No Hit
TGCTAAGACTTCACCAGTCAAAGCGAACTACTATACTCAATTG	988	0.10096385739161713	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	3.065704171810237E-4	0.0	0.0
2	1.0219013906034123E-4	0.0	3.065704171810237E-4	0.0	0.0
3	1.0219013906034123E-4	0.0	3.065704171810237E-4	0.0	0.0
4	1.0219013906034123E-4	0.0	6.131408343620474E-4	1.0219013906034123E-4	0.0
5	1.0219013906034123E-4	0.0	9.197112515430711E-4	1.0219013906034123E-4	0.0
6	1.0219013906034123E-4	0.0	0.0015328520859051184	1.0219013906034123E-4	0.0
7	1.0219013906034123E-4	0.0	0.0035766548671119433	3.065704171810237E-4	0.0
8	2.0438027812068245E-4	0.0	0.004394175979594673	3.065704171810237E-4	0.0
9	2.0438027812068245E-4	0.0	0.006029218204560133	0.0012262816687240948	0.0
10	5.109506953017062E-4	0.0	0.006437978760801498	0.0014306619468447772	0.0
11	5.109506953017062E-4	0.0	0.009503682932611736	0.001737232364025801	0.0
12	5.109506953017062E-4	0.0	0.010832154740396172	0.0018394225030861423	0.0
13	5.109506953017062E-4	0.0	0.011649675852878901	0.0018394225030861423	0.0
14	5.109506953017062E-4	0.0	0.012569387104421972	0.0019416126421464835	0.0
15	7.153309734223886E-4	0.0	0.014408809607508113	0.002043802781206825	0.0
16	7.153309734223886E-4	0.0	0.015532901137171868	0.002043802781206825	0.0
17	7.153309734223886E-4	0.0	0.01583947155435289	0.002145992920267166	0.0
18	7.153309734223886E-4	0.0	0.016248232110594257	0.0022481830593275073	0.0
19	7.153309734223886E-4	0.0	0.016759182805895962	0.002656943615568872	0.0
20	7.153309734223886E-4	0.0	0.01716794336213733	0.0027591337546292133	0.0
21	8.175211124827298E-4	0.0	0.017781084196499375	0.0032700844499309193	0.0
22	8.175211124827298E-4	0.0	0.017781084196499375	0.00408760556241365	0.0
23	8.175211124827298E-4	0.0	0.017883274335559717	0.005109506953017062	0.0
24	8.175211124827298E-4	0.0	0.017883274335559717	0.006029218204560133	0.0
25	8.175211124827298E-4	0.0	0.017883274335559717	0.0065401688998618385	0.0
26	8.175211124827298E-4	0.0	0.017883274335559717	0.007255499873284227	0.0
27	9.197112515430711E-4	0.0	0.017985464474620058	0.008379591402947981	0.0
28	9.197112515430711E-4	0.0	0.017985464474620058	0.017985464474620058	0.0
29	9.197112515430711E-4	0.0	0.017985464474620058	0.034642457141455675	0.0
30	9.197112515430711E-4	0.0	0.017985464474620058	0.062335984826808154	0.0
31	9.197112515430711E-4	0.0	0.017985464474620058	0.12722672313012484	0.0
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GGTATCA	1170	0.0	24.350426	1
GTATCAA	2780	0.0	20.895683	1
GCCGGCA	180	0.0	20.555555	15
TAATTCG	45	0.0038254077	20.555555	5
TAGGACC	315	0.0	19.968254	4
GGACCGT	75	9.263464E-6	19.733334	6
AGGACCG	70	1.2189624E-4	18.5	5
CGCCGTT	60	9.23548E-4	18.5	25
TGCGGTA	135	1.1514203E-9	17.814814	36
GGACCCT	115	6.401933E-8	17.69565	6
GTTTAGG	235	0.0	17.319149	1
CGGAATA	75	2.066871E-4	17.266666	27
ATTTCGG	215	0.0	17.209303	29
CCGGCAG	205	0.0	17.146343	16
GCCGCTC	185	1.8189894E-12	17.0	27
TGGGGCG	210	0.0	16.738094	37
TTGCGGT	155	4.0017767E-10	16.709679	35
AGCTTCG	205	0.0	16.243904	21
CGGTTGG	240	0.0	16.1875	33
GTCTTAT	70	0.0025921247	15.857142	1
>>END_MODULE