FastQCFastQC Report
Fri 10 Feb 2017
ERR1633287.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1633287.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences926828
Sequences flagged as poor quality0
Sequence length43
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT70610.7618457793679086No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT50730.5473507490062881No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT26170.2823609127044069No Hit
GCTTTATTGAGCCTGTGTGGGAGCAGGGAGCAAGCTTTGGCCA18510.19971343118680057No Hit
ATCATTAACTGAATCCATAGGTTAATGAGGCGAACCGGGGGAA13070.14101861402547183No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT12890.13907650610469258No Hit
GTACTGGTTCACTATCGGTCAGTCAGGAGTATTTAGCCTTGGA11480.1238633273919217No Hit
CCGTATTACCGCGGCTGCTGGCACGGAGTTAGCCGGTGCTTCT10120.1091896231015895No Hit
GTATGAAACTGCTCGCCGGCAGCTTCGCCGCTCTCTTCCTGAG9970.10757119983427346No Hit
GTTAATGATAGTGTGTCGAAACACACTGGGTTTCCCCATTCGG9450.10196066584091115No Hit
GTGTGGGAGCAGGGAGCAAGCTTTGGCCAGAGCCAAGGGTGCA9350.1008817169960338No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA12400.025.213711
GTATCAA34900.024.0128961
TTCGCCG2450.020.38775424
GCGGTAT555.142062E-420.1818181
TAGACTA656.900287E-519.9230775
CCGGCAG2800.019.16071316
GCCGGCA2800.019.16071315
CGAGTCG2350.018.89361821
GCGGCGC500.007033878518.511
TTACCGG701.21887424E-418.531
AATCGTA500.007033878518.56
AGCTTCG3050.018.1967221
GCTTCGC2950.018.18644122
GCCGCTC2850.018.17543827
CCGGTTA1156.400842E-817.6956523
AGGACCG1054.7947833E-717.6190475
TTTACCG852.722195E-517.41176430
GCCGTCC2450.017.36734627
AACGTAT752.0667216E-417.26666615
GAGTCGC2700.017.1296322
Produced by FastQC (version 0.11.5)