##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	ERR1633287.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	926828
Sequences flagged as poor quality	0
Sequence length	43
%GC	46
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.734188004678323	33.0	33.0	33.0	27.0	33.0
2	31.877242595174078	33.0	33.0	33.0	33.0	33.0
3	31.607235646743515	33.0	33.0	33.0	27.0	33.0
4	31.902111287099657	33.0	33.0	33.0	33.0	33.0
5	32.00073260626567	33.0	33.0	33.0	33.0	33.0
6	35.14591380493468	37.0	37.0	37.0	33.0	37.0
7	35.31183563724877	37.0	37.0	37.0	33.0	37.0
8	35.43962202264066	37.0	37.0	37.0	33.0	37.0
9	35.557554368232296	37.0	37.0	37.0	33.0	37.0
10	35.5525825719551	37.0	37.0	37.0	33.0	37.0
11	35.58818464698952	37.0	37.0	37.0	33.0	37.0
12	35.57426404899291	37.0	37.0	37.0	33.0	37.0
13	35.56536056312498	37.0	37.0	37.0	33.0	37.0
14	35.55741734172899	37.0	37.0	37.0	33.0	37.0
15	35.5822698494219	37.0	37.0	37.0	33.0	37.0
16	35.56630895915963	37.0	37.0	37.0	33.0	37.0
17	35.545268377735674	37.0	37.0	37.0	33.0	37.0
18	35.53885510580172	37.0	37.0	37.0	33.0	37.0
19	35.517144497145104	37.0	37.0	37.0	33.0	37.0
20	35.520269132999864	37.0	37.0	37.0	33.0	37.0
21	35.50576698157587	37.0	37.0	37.0	33.0	37.0
22	35.41294717034876	37.0	37.0	37.0	33.0	37.0
23	35.454755359138915	37.0	37.0	37.0	33.0	37.0
24	35.45143867038976	37.0	37.0	37.0	33.0	37.0
25	35.48134065867669	37.0	37.0	37.0	33.0	37.0
26	35.362270022053714	37.0	37.0	37.0	33.0	37.0
27	35.37731596369553	37.0	37.0	37.0	33.0	37.0
28	35.39388430215747	37.0	37.0	37.0	33.0	37.0
29	35.38975084913274	37.0	37.0	37.0	33.0	37.0
30	35.40510429119534	37.0	37.0	37.0	33.0	37.0
31	35.39479601393139	37.0	37.0	37.0	33.0	37.0
32	35.37684985779454	37.0	37.0	37.0	33.0	37.0
33	35.362239811486056	37.0	37.0	37.0	33.0	37.0
34	35.32986918824205	37.0	37.0	37.0	33.0	37.0
35	35.26543652112366	37.0	37.0	37.0	33.0	37.0
36	35.289259711618556	37.0	37.0	37.0	33.0	37.0
37	35.285500653843	37.0	37.0	37.0	33.0	37.0
38	35.26184362147022	37.0	37.0	37.0	33.0	37.0
39	35.15701079380425	37.0	37.0	37.0	33.0	37.0
40	34.9393609170202	37.0	37.0	37.0	27.0	37.0
41	35.09504568269409	37.0	37.0	37.0	27.0	37.0
42	35.125388961058576	37.0	37.0	37.0	33.0	37.0
43	34.77842059152292	37.0	37.0	37.0	27.0	37.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
14	6.0
15	28.0
16	47.0
17	47.0
18	36.0
19	31.0
20	74.0
21	166.0
22	528.0
23	1174.0
24	2310.0
25	4096.0
26	6674.0
27	9787.0
28	13883.0
29	18300.0
30	24034.0
31	31066.0
32	39206.0
33	51412.0
34	73669.0
35	147714.0
36	502540.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	42.48328708239285	19.61518210498604	14.217416823833547	23.684113988787566
2	16.040408792138347	22.353014798862354	38.571126465752	23.03544994324729
3	18.40524887034056	27.196847743054807	30.421825840393257	23.97607754621138
4	12.376406409819298	18.065487879088675	39.831554506337746	29.72655120475428
5	14.391667062281243	36.32087075487577	34.35524174927818	14.932220433564805
6	28.592575968788168	40.21943661607116	17.285731548895804	13.90225586624487
7	26.13365155131265	32.704773701269275	22.766360101334875	18.3952146460832
8	25.290560923925476	34.076657157530846	20.72908889243743	19.903693026106247
9	27.068884410052352	15.104096984553768	19.862261390462955	37.96475721493093
10	15.419257942142448	28.049109435623436	32.883879209518916	23.647753412715197
11	34.00091494862046	24.180646247200126	23.706124545223062	18.112314258956356
12	23.755971981856398	25.718364140919352	29.901664602277876	20.623999274946375
13	29.560285187758677	22.135175027081615	25.555334970458382	22.749204814701326
14	22.725036360576073	21.2546448747772	28.45749157340952	27.56282719123721
15	24.221322618652007	28.3855256854562	24.87473403910974	22.518417656782056
16	22.117588160910113	28.26748868182662	25.567958671943448	24.046964485319823
17	22.817718066351038	26.828386712529184	26.212522711873188	24.14137250924659
18	24.23146473779385	25.38852947904034	28.13963324370865	22.24037253945716
19	26.018311919795256	25.444203239436014	27.83547756433772	20.702007276431008
20	25.869740663855644	25.138213347028792	27.212816185958992	21.77922980315657
21	23.932919592416287	26.0724751518081	27.685503674899763	22.309101580875847
22	23.15801853202536	26.162567380355362	28.2552965598795	22.424117527739774
23	23.265266047206172	25.71415624042433	28.134346394368748	22.886231318000753
24	22.780278541433795	25.86434591963126	28.421454681990614	22.933920856944333
25	23.22178440875761	26.351815007746854	28.278062380506412	22.14833820298912
26	23.488500563211296	26.064814615009475	27.699530009883173	22.74715481189606
27	23.347050369647874	26.05952776566958	28.20728333628246	22.38613852840009
28	23.089073700837695	26.2695991057672	27.561964032161306	23.079363161233797
29	23.249837078724422	26.44827303447889	27.569732463844428	22.732157422952262
30	23.797943091922125	26.158683164513803	27.62238516747444	22.42098857608963
31	23.201068590935968	26.098154134316182	27.76750378711045	22.933273487637404
32	22.397791175924766	26.272080688110417	28.026127825227547	23.304000310737266
33	22.351072690941574	26.411372983984084	28.413686250307503	22.82386807476684
34	23.002865688131994	25.82474849702426	27.730711631500128	23.44167418334362
35	22.616170422127947	26.30045704273069	27.816595959552366	23.266776575589
36	22.979020918660204	26.221046407747718	28.132080601794506	22.667852071797572
37	23.672029761724936	25.887651214680606	27.940567181828772	22.49975184176568
38	23.391826746710283	26.211120078374844	27.409724350149112	22.98732882476576
39	23.14701325380761	25.74264048992909	27.814114377209147	23.29623187905415
40	22.893028695723476	25.94774866534028	28.109854255590033	23.04936838334621
41	22.9993051569439	25.787308972107013	27.900538179683824	23.31284769126526
42	22.65695468846431	26.344478155601685	28.00411726879205	22.99444988714195
43	22.985602506613954	26.3443702607172	27.753693241896016	22.91633399077283
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	51.0
1	87.5
2	124.0
3	410.0
4	696.0
5	696.0
6	838.5
7	981.0
8	968.0
9	955.0
10	1280.0
11	1605.0
12	1605.0
13	2559.0
14	3513.0
15	6323.0
16	9133.0
17	10271.0
18	11409.0
19	11409.0
20	10221.0
21	9033.0
22	9661.5
23	10290.0
24	12834.0
25	15378.0
26	15378.0
27	18426.0
28	21474.0
29	25021.5
30	28569.0
31	32297.5
32	36026.0
33	36026.0
34	40121.0
35	44216.0
36	46331.0
37	48446.0
38	49911.5
39	51377.0
40	51377.0
41	54588.5
42	57800.0
43	59044.5
44	60289.0
45	62592.0
46	64895.0
47	64895.0
48	66734.0
49	68573.0
50	68925.5
51	69278.0
52	68552.5
53	67827.0
54	67827.0
55	62602.0
56	57377.0
57	52580.5
58	47784.0
59	45142.5
60	42501.0
61	42501.0
62	37803.5
63	33106.0
64	27744.0
65	22382.0
66	19109.5
67	15837.0
68	15837.0
69	13349.5
70	10862.0
71	9133.0
72	7404.0
73	5501.0
74	3598.0
75	3598.0
76	2578.0
77	1558.0
78	1209.5
79	861.0
80	724.5
81	588.0
82	588.0
83	498.5
84	409.0
85	371.5
86	334.0
87	242.5
88	151.0
89	151.0
90	127.5
91	104.0
92	68.5
93	33.0
94	17.0
95	1.0
96	1.0
97	0.5
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
43	926828.0
>>END_MODULE
>>Sequence Duplication Levels	warn
#Total Deduplicated Percentage	61.41332825608677
#Duplication Level	Percentage of deduplicated	Percentage of total
1	87.34750686343963	53.643011113552106
2	7.800413892603582	9.580987578396067
3	1.8083219414522254	3.331652069492689
4	0.7900248917290086	1.9407223202493207
5	0.44354823006776944	1.361988652527911
6	0.29227909883165826	1.0769899343365115
7	0.19597058329765163	0.8424644032416834
8	0.15124509054482896	0.7430771514200917
9	0.12299073627368669	0.6797943413310348
>10	0.8698620996731682	10.582910141091741
>50	0.10695337491083241	4.596048745737838
>100	0.06590893776836848	7.816400404520578
>500	0.003553042434034291	1.4737509054820221
>1k	0.001065912730210287	1.0063575919578156
>5k	3.5530424340342906E-4	1.3238446466626337
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT	7061	0.7618457793679086	No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT	5073	0.5473507490062881	No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT	2617	0.2823609127044069	No Hit
GCTTTATTGAGCCTGTGTGGGAGCAGGGAGCAAGCTTTGGCCA	1851	0.19971343118680057	No Hit
ATCATTAACTGAATCCATAGGTTAATGAGGCGAACCGGGGGAA	1307	0.14101861402547183	No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	1289	0.13907650610469258	No Hit
GTACTGGTTCACTATCGGTCAGTCAGGAGTATTTAGCCTTGGA	1148	0.1238633273919217	No Hit
CCGTATTACCGCGGCTGCTGGCACGGAGTTAGCCGGTGCTTCT	1012	0.1091896231015895	No Hit
GTATGAAACTGCTCGCCGGCAGCTTCGCCGCTCTCTTCCTGAG	997	0.10757119983427346	No Hit
GTTAATGATAGTGTGTCGAAACACACTGGGTTTCCCCATTCGG	945	0.10196066584091115	No Hit
GTGTGGGAGCAGGGAGCAAGCTTTGGCCAGAGCCAAGGGTGCA	935	0.1008817169960338	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	3.2368465346321E-4	0.0	0.0
2	1.0789488448773667E-4	0.0	6.4736930692642E-4	1.0789488448773667E-4	0.0
3	1.0789488448773667E-4	0.0	7.552641914141567E-4	2.1578976897547333E-4	0.0
4	1.0789488448773667E-4	0.0	0.0010789488448773668	2.1578976897547333E-4	0.0
5	2.1578976897547333E-4	0.0	0.00161842326731605	2.1578976897547333E-4	0.0
6	2.1578976897547333E-4	0.0	0.004639480032972676	2.1578976897547333E-4	0.0
7	2.1578976897547333E-4	0.0	0.00938685495043309	4.3157953795094667E-4	0.0
8	3.2368465346321E-4	0.0	0.010573698679798193	4.3157953795094667E-4	0.0
9	3.2368465346321E-4	0.0	0.01230001683160198	8.631590759018933E-4	0.0
10	7.552641914141567E-4	0.0	0.014134229867893503	9.710539603896301E-4	0.0
11	7.552641914141567E-4	0.0	0.021255292244084123	0.0010789488448773668	0.0
12	7.552641914141567E-4	0.0	0.023197400164863383	0.0010789488448773668	0.0
13	7.552641914141567E-4	0.0	0.02427634900974075	0.0011868437293651034	0.0
14	7.552641914141567E-4	0.0	0.025463192739105852	0.00129473861385284	0.0
15	7.552641914141567E-4	0.0	0.02783688019783606	0.0014026334983405766	0.0
16	7.552641914141567E-4	0.0	0.031073726732468162	0.0014026334983405766	0.0
17	7.552641914141567E-4	0.0	0.032152675577345524	0.0014026334983405766	0.0
18	7.552641914141567E-4	0.0	0.03387899372914931	0.00161842326731605	0.0
19	7.552641914141567E-4	0.0	0.03452636303607574	0.00161842326731605	0.0
20	7.552641914141567E-4	0.0	0.03592899653441631	0.00161842326731605	0.0
21	7.552641914141567E-4	0.0	0.03711584026378141	0.0018342130362915233	0.0
22	7.552641914141567E-4	0.0	0.03787110445519557	0.002373687458730207	0.0
23	7.552641914141567E-4	0.0	0.038302683993146514	0.0034526363036075734	0.0
24	7.552641914141567E-4	0.0	0.03851847376212199	0.0041000056105339935	0.0
25	7.552641914141567E-4	0.0	0.03873426353109746	0.0044236902639972035	0.0
26	7.552641914141567E-4	0.0	0.03873426353109746	0.004963164686435886	0.0
27	7.552641914141567E-4	0.0	0.03873426353109746	0.007336852145166093	0.0
28	7.552641914141567E-4	0.0	0.03873426353109746	0.01661581221111145	0.0
29	7.552641914141567E-4	0.0	0.03873426353109746	0.04542374636933714	0.0
30	7.552641914141567E-4	0.0	0.03873426353109746	0.08081326848131476	0.0
31	7.552641914141567E-4	0.0	0.03873426353109746	0.15731074158312006	0.0
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GGTATCA	1240	0.0	25.21371	1
GTATCAA	3490	0.0	24.012896	1
TTCGCCG	245	0.0	20.387754	24
GCGGTAT	55	5.142062E-4	20.181818	1
TAGACTA	65	6.900287E-5	19.923077	5
CCGGCAG	280	0.0	19.160713	16
GCCGGCA	280	0.0	19.160713	15
CGAGTCG	235	0.0	18.893618	21
GCGGCGC	50	0.0070338785	18.5	11
TTACCGG	70	1.21887424E-4	18.5	31
AATCGTA	50	0.0070338785	18.5	6
AGCTTCG	305	0.0	18.19672	21
GCTTCGC	295	0.0	18.186441	22
GCCGCTC	285	0.0	18.175438	27
CCGGTTA	115	6.400842E-8	17.69565	23
AGGACCG	105	4.7947833E-7	17.619047	5
TTTACCG	85	2.722195E-5	17.411764	30
GCCGTCC	245	0.0	17.367346	27
AACGTAT	75	2.0667216E-4	17.266666	15
GAGTCGC	270	0.0	17.12963	22
>>END_MODULE