FastQCFastQC Report
Fri 10 Feb 2017
ERR1633286.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1633286.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences840125
Sequences flagged as poor quality0
Sequence length43
%GC43

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT60540.7206070525219461No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT42520.5061151614343105No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT24810.29531319744085704No Hit
ATTGAAAGCTGAGTATTTTTAAGACAAAGGTTTCAGGAAGAAA11090.13200416604671922No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT10530.1253384912959381No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGGAACT400.001930756623.1250024
GGTATCA12600.022.317461
GTATCAA28850.021.4818021
CCGACAA450.00382496420.55555514
TCGCCAT1303.274181E-1119.92307713
GTCGCCA1355.638867E-1119.18518612
GTTACCG500.00703334618.4999985
GTATTAG2600.017.7884621
CGAGACA752.066432E-417.26666623
ATAGGTC650.001579455917.0769233
TGCGGGT1005.8740043E-616.6521
GGACCGT904.4436656E-516.4444456
TCGGTTG803.3812094E-416.187532
GCCGCTC1151.2411456E-616.08695627
TTCGCCG1151.2411456E-616.08695624
GGCCGCA1751.3096724E-1015.85714333
ACGGGTA1403.468631E-815.85714216
GCCGCGT1201.9338931E-615.41666736
TAATACT1701.4824764E-915.2352934
GGTCGGA855.362018E-415.2352931
Produced by FastQC (version 0.11.5)