##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	ERR1633286.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	840125
Sequences flagged as poor quality	0
Sequence length	43
%GC	43
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.745866388930217	33.0	33.0	33.0	27.0	33.0
2	31.885567028715965	33.0	33.0	33.0	33.0	33.0
3	31.60550156226752	33.0	33.0	33.0	27.0	33.0
4	31.89144115459009	33.0	33.0	33.0	33.0	33.0
5	32.00348876655259	33.0	33.0	33.0	33.0	33.0
6	35.13966850171106	37.0	37.0	37.0	33.0	37.0
7	35.308756435054306	37.0	37.0	37.0	33.0	37.0
8	35.44245201606904	37.0	37.0	37.0	33.0	37.0
9	35.55514834102068	37.0	37.0	37.0	33.0	37.0
10	35.566072905817585	37.0	37.0	37.0	33.0	37.0
11	35.57918166939444	37.0	37.0	37.0	33.0	37.0
12	35.579312602291324	37.0	37.0	37.0	33.0	37.0
13	35.57494182413331	37.0	37.0	37.0	33.0	37.0
14	35.54816009522393	37.0	37.0	37.0	33.0	37.0
15	35.562857908049395	37.0	37.0	37.0	33.0	37.0
16	35.54934563309031	37.0	37.0	37.0	33.0	37.0
17	35.540511233447404	37.0	37.0	37.0	33.0	37.0
18	35.529977086743045	37.0	37.0	37.0	33.0	37.0
19	35.5250052075584	37.0	37.0	37.0	33.0	37.0
20	35.52807141794376	37.0	37.0	37.0	33.0	37.0
21	35.522576997470615	37.0	37.0	37.0	33.0	37.0
22	35.424024996280316	37.0	37.0	37.0	33.0	37.0
23	35.47030858503199	37.0	37.0	37.0	33.0	37.0
24	35.46260973069484	37.0	37.0	37.0	33.0	37.0
25	35.47915369736646	37.0	37.0	37.0	33.0	37.0
26	35.36859395923226	37.0	37.0	37.0	33.0	37.0
27	35.3833405743193	37.0	37.0	37.0	33.0	37.0
28	35.40029043297128	37.0	37.0	37.0	33.0	37.0
29	35.40990685909835	37.0	37.0	37.0	33.0	37.0
30	35.40896057134355	37.0	37.0	37.0	33.0	37.0
31	35.40176878440708	37.0	37.0	37.0	33.0	37.0
32	35.38292277934831	37.0	37.0	37.0	33.0	37.0
33	35.384465406933494	37.0	37.0	37.0	33.0	37.0
34	35.34848861776521	37.0	37.0	37.0	33.0	37.0
35	35.28252581461092	37.0	37.0	37.0	33.0	37.0
36	35.30820651688737	37.0	37.0	37.0	33.0	37.0
37	35.303953578336554	37.0	37.0	37.0	33.0	37.0
38	35.28917720577295	37.0	37.0	37.0	33.0	37.0
39	35.185421217080794	37.0	37.0	37.0	33.0	37.0
40	34.967590537122454	37.0	37.0	37.0	27.0	37.0
41	35.11644338640083	37.0	37.0	37.0	27.0	37.0
42	35.1639029906264	37.0	37.0	37.0	33.0	37.0
43	34.798076476714776	37.0	37.0	37.0	27.0	37.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
14	7.0
15	32.0
16	51.0
17	52.0
18	33.0
19	39.0
20	59.0
21	177.0
22	423.0
23	1021.0
24	2058.0
25	3731.0
26	5981.0
27	9022.0
28	12454.0
29	16901.0
30	22091.0
31	27719.0
32	35045.0
33	46055.0
34	65894.0
35	132717.0
36	458563.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	41.36610623419134	21.12578485344443	14.326439517928879	23.181669394435353
2	15.307722065168875	22.79696473739027	40.09950900163666	21.795804195804198
3	17.616069037345632	28.2905520011903	31.292724296979618	22.80065466448445
4	12.11153102216932	18.411307841095077	40.77107573277786	28.706085403957744
5	13.186490105639043	37.90959678619253	35.39366165749144	13.510251450676982
6	27.521261717006396	41.33015920249963	17.790566879928583	13.358012200565392
7	24.762148489808062	33.72914744829639	24.040589198036006	17.468114863859544
8	23.9607201309329	36.174735902395476	21.148638595447107	18.71590537122452
9	25.86591281059366	15.697306948370779	21.03115607796459	37.405624163070975
10	14.327867876804046	28.781193274810295	34.513435500669544	22.377503347716114
11	32.453861032584435	24.89105787829192	24.693706293706295	17.96137479541735
12	22.14289540246987	26.90432971284035	31.232019044785	19.720755839904776
13	27.752417794970984	23.214759708376732	27.12691563755393	21.905906859098348
14	21.6072608242821	22.248593959232256	29.365987204285076	26.778158012200564
15	23.284630263353666	29.417051034072312	25.984109507513764	21.31420919506026
16	21.49846748995685	29.756048207112034	26.198363338788873	22.54712096414224
17	21.449189108763576	28.281029608689185	27.31426871001339	22.95551257253385
18	22.29001636661211	27.182026484154143	29.25957446808511	21.26838268114864
19	23.953816396369586	26.766612111292964	29.459544710608544	19.82002678172891
20	23.758369290284183	26.421306353221247	29.30480583246541	20.51551852402916
21	22.06778753161732	27.839547686356198	28.784407082279422	21.30825769974706
22	21.633923523285226	27.72325546793632	29.00913554530576	21.6336854634727
23	21.619520904627286	27.394494866835295	29.423478649010566	21.562505579526857
24	22.162416307097157	27.640529683082875	28.76905222437137	21.428001785448593
25	22.087070376432077	27.26070525219461	29.4993006993007	21.152923672072607
26	22.01565243267371	27.481386698407974	28.969974706144917	21.532986162773398
27	21.846748995685168	27.556970688885585	28.871536973664636	21.72474334176462
28	21.334206219312602	27.645052819520906	29.24814759708377	21.772593364082727
29	22.417259336408275	27.549709864603482	28.62454991816694	21.40848088082131
30	21.927808361850914	27.764677875316174	29.156851658979317	21.150662103853595
31	22.18265139116203	27.280583246540697	29.17161136735605	21.36515399494123
32	21.029251599464367	27.400565392054755	29.305043892277933	22.265139116202946
33	21.256598720428507	27.34474036601696	29.86008034518673	21.538580568367802
34	21.42574021722958	27.353905668799282	29.13602142538313	22.084332688588006
35	21.7356940931409	28.02309180181521	28.810236571938702	21.430977533105192
36	22.09587858949561	27.673739026930516	28.717512274959084	21.51287010861479
37	22.3078708525517	27.1970242523434	28.518970391310816	21.97613450379408
38	22.122779348311266	27.739086445469425	28.70894212170808	21.429192084511232
39	21.882576997470615	27.327123939889898	29.00282696027377	21.78747210236572
40	21.836393393840204	27.639577443832763	29.05769974706145	21.466329415265587
41	21.680107126915637	27.244160095223926	29.198036006546644	21.877696771313794
42	21.478113375985718	27.425204582651393	29.07817289093885	22.01850915042404
43	21.545365273024846	27.326171700639783	28.98640083320934	22.142062193126023
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	52.0
1	65.0
2	78.0
3	337.5
4	597.0
5	597.0
6	729.5
7	862.0
8	836.5
9	811.0
10	1123.5
11	1436.0
12	1436.0
13	2307.5
14	3179.0
15	5912.5
16	8646.0
17	9925.5
18	11205.0
19	11205.0
20	10769.0
21	10333.0
22	11423.5
23	12514.0
24	15609.0
25	18704.0
26	18704.0
27	21956.5
28	25209.0
29	30791.0
30	36373.0
31	39013.0
32	41653.0
33	41653.0
34	44958.0
35	48263.0
36	50426.5
37	52590.0
38	54988.5
39	57387.0
40	57387.0
41	59416.5
42	61446.0
43	59767.5
44	58089.0
45	57981.0
46	57873.0
47	57873.0
48	56962.0
49	56051.0
50	56766.0
51	57481.0
52	56914.0
53	56347.0
54	56347.0
55	50925.0
56	45503.0
57	40380.0
58	35257.0
59	31030.5
60	26804.0
61	26804.0
62	23084.5
63	19365.0
64	15885.0
65	12405.0
66	10451.5
67	8498.0
68	8498.0
69	7076.5
70	5655.0
71	4828.5
72	4002.0
73	3219.5
74	2437.0
75	2437.0
76	1816.0
77	1195.0
78	904.0
79	613.0
80	507.0
81	401.0
82	401.0
83	348.5
84	296.0
85	265.5
86	235.0
87	199.0
88	163.0
89	163.0
90	123.0
91	83.0
92	56.0
93	29.0
94	17.0
95	5.0
96	5.0
97	2.5
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
43	840125.0
>>END_MODULE
>>Sequence Duplication Levels	warn
#Total Deduplicated Percentage	60.54136950122354
#Duplication Level	Percentage of deduplicated	Percentage of total
1	88.38115200490707	53.50715980472882
2	7.020967346582972	8.501179567710093
3	1.5919055771891515	2.8912843127900087
4	0.6925746035677175	1.6771765992702636
5	0.40659306640479753	1.230785053492418
6	0.2793453786198106	1.0147171071288688
7	0.19150637284324346	0.8115840656099318
8	0.16263970482076098	0.7877144372098894
9	0.12113899802599275	0.6600528756449653
>10	0.9252722240494818	11.479655890499782
>50	0.14390624782957298	6.078572339145561
>100	0.07981388139015319	8.747079945074365
>500	0.002189408216391825	0.8116866026939105
>1k	7.961484423243E-4	1.0718456548340756
>5k	1.99037110581075E-4	0.7295057441670032
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT	6054	0.7206070525219461	No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT	4252	0.5061151614343105	No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT	2481	0.29531319744085704	No Hit
ATTGAAAGCTGAGTATTTTTAAGACAAAGGTTTCAGGAAGAAA	1109	0.13200416604671922	No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	1053	0.1253384912959381	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	1.1902990626394882E-4	0.0	0.0
2	0.0	0.0	2.3805981252789763E-4	1.1902990626394882E-4	0.0
3	0.0	0.0	3.5708971879184646E-4	2.3805981252789763E-4	0.0
4	0.0	0.0	4.7611962505579526E-4	2.3805981252789763E-4	0.0
5	2.3805981252789763E-4	0.0	7.141794375836929E-4	2.3805981252789763E-4	0.0
6	2.3805981252789763E-4	0.0	0.0010712691563755394	3.5708971879184646E-4	0.0
7	2.3805981252789763E-4	0.0	0.0034518672816545156	5.951495313197441E-4	0.0
8	3.5708971879184646E-4	0.0	0.003927986906710311	5.951495313197441E-4	0.0
9	3.5708971879184646E-4	0.0	0.0049992560630858504	0.0011902990626394881	0.0
10	3.5708971879184646E-4	0.0	0.006070525219461389	0.001309328968903437	0.0
11	3.5708971879184646E-4	0.0	0.009879482219907752	0.0014283588751673858	0.0
12	3.5708971879184646E-4	0.0	0.010355601844963548	0.0014283588751673858	0.0
13	3.5708971879184646E-4	0.0	0.011307841095075138	0.0015473887814313346	0.0
14	3.5708971879184646E-4	0.0	0.01190299062639488	0.0016664186876952833	0.0
15	3.5708971879184646E-4	0.0	0.012974259782770422	0.0016664186876952833	0.0
16	3.5708971879184646E-4	0.0	0.013688439220354114	0.0017854485939592323	0.0
17	3.5708971879184646E-4	0.0	0.013688439220354114	0.001904478500223181	0.0
18	3.5708971879184646E-4	0.0	0.014283588751673858	0.00202350840648713	0.0
19	3.5708971879184646E-4	0.0	0.014759708376729653	0.0027376878440708227	0.0
20	3.5708971879184646E-4	0.0	0.015354857908049397	0.0027376878440708227	0.0
21	3.5708971879184646E-4	0.0	0.015592917720577295	0.00297574765659872	0.0
22	3.5708971879184646E-4	0.0	0.01583097753310519	0.003808957000446362	0.0
23	3.5708971879184646E-4	0.0	0.01595000743936914	0.0045231364380300555	0.0
24	3.5708971879184646E-4	0.0	0.01595000743936914	0.0047611962505579525	0.0
25	4.7611962505579526E-4	0.0	0.01595000743936914	0.0054753756881416454	0.0
26	4.7611962505579526E-4	0.0	0.01595000743936914	0.006546644844517185	0.0
27	4.7611962505579526E-4	0.0	0.01595000743936914	0.007855973813420621	0.0
28	4.7611962505579526E-4	0.0	0.01595000743936914	0.016783216783216783	0.0
29	7.141794375836929E-4	0.0	0.01595000743936914	0.03582800178544859	0.0
30	7.141794375836929E-4	0.0	0.01595000743936914	0.06570450825769975	0.0
31	7.141794375836929E-4	0.0	0.01595000743936914	0.13248028567177503	0.0
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
CGGAACT	40	0.0019307566	23.125002	4
GGTATCA	1260	0.0	22.31746	1
GTATCAA	2885	0.0	21.481802	1
CCGACAA	45	0.003824964	20.555555	14
TCGCCAT	130	3.274181E-11	19.923077	13
GTCGCCA	135	5.638867E-11	19.185186	12
GTTACCG	50	0.007033346	18.499998	5
GTATTAG	260	0.0	17.788462	1
CGAGACA	75	2.066432E-4	17.266666	23
ATAGGTC	65	0.0015794559	17.076923	3
TGCGGGT	100	5.8740043E-6	16.65	21
GGACCGT	90	4.4436656E-5	16.444445	6
TCGGTTG	80	3.3812094E-4	16.1875	32
GCCGCTC	115	1.2411456E-6	16.086956	27
TTCGCCG	115	1.2411456E-6	16.086956	24
GGCCGCA	175	1.3096724E-10	15.857143	33
ACGGGTA	140	3.468631E-8	15.857142	16
GCCGCGT	120	1.9338931E-6	15.416667	36
TAATACT	170	1.4824764E-9	15.235293	4
GGTCGGA	85	5.362018E-4	15.235293	1
>>END_MODULE