FastQCFastQC Report
Fri 10 Feb 2017
ERR1633285.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1633285.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences337953
Sequences flagged as poor quality0
Sequence length43
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT13600.4024228221083997No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT10900.3225300559545262No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT6290.18612055522513485No Hit
GTACATGCTAAGACTTCACCAGTCAAAGCGAACTACTATACTC3670.10859498214248726No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CCGTCCG250.00549092229.628
CGTCCGC250.00549092229.629
GGTATCA3400.027.2058831
TGAGTAC358.8563905E-426.428573
GGCGGAA358.8563905E-426.4285726
CGAGACT400.001928382823.1254
GCGGAAT400.001928382823.12527
CGGAATA450.003820303820.55555728
TCCGCCG450.003820303820.55555731
CGCCCGA450.003820303820.55555710
AGGTCGC909.4287316E-820.55555735
GTACTAA555.132775E-420.1818181
GTATCAA8000.020.1187521
GTCGCCC951.6673766E-719.47368437
GGTCGCC951.6673766E-719.47368436
GCTCCGA951.6673766E-719.47368429
CGAACTA1254.0927262E-1019.2429
GCGAACT1355.4569682E-1119.18518428
TTAGGCC500.007024859518.55
TTGGCGG500.007024859518.524
Produced by FastQC (version 0.11.5)