##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	ERR1633285.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	337953
Sequences flagged as poor quality	0
Sequence length	43
%GC	44
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	30.73031752936059	33.0	33.0	33.0	27.0	33.0
2	31.144552644894407	33.0	33.0	33.0	27.0	33.0
3	30.905557873432105	33.0	33.0	33.0	27.0	33.0
4	31.221371019047027	33.0	33.0	33.0	27.0	33.0
5	31.351350631596702	33.0	33.0	33.0	27.0	33.0
6	33.604678757105276	37.0	33.0	37.0	27.0	37.0
7	33.826029063212935	37.0	33.0	37.0	27.0	37.0
8	34.00241749592399	37.0	33.0	37.0	27.0	37.0
9	34.03501374451477	37.0	33.0	37.0	27.0	37.0
10	34.0142445843061	37.0	33.0	37.0	27.0	37.0
11	34.07629759167695	37.0	33.0	37.0	27.0	37.0
12	34.13787124245087	37.0	33.0	37.0	27.0	37.0
13	34.15555713368427	37.0	33.0	37.0	27.0	37.0
14	34.17124274677248	37.0	33.0	37.0	27.0	37.0
15	34.17653342328667	37.0	33.0	37.0	27.0	37.0
16	34.1384038608919	37.0	33.0	37.0	27.0	37.0
17	34.20412009954047	37.0	33.0	37.0	27.0	37.0
18	34.16139226460484	37.0	33.0	37.0	27.0	37.0
19	34.11277011892186	37.0	33.0	37.0	27.0	37.0
20	34.12525410338124	37.0	33.0	37.0	27.0	37.0
21	34.14187179874124	37.0	33.0	37.0	27.0	37.0
22	34.024947255979384	37.0	33.0	37.0	27.0	37.0
23	34.06158548673928	37.0	33.0	37.0	27.0	37.0
24	34.05798143528834	37.0	33.0	37.0	27.0	37.0
25	34.05269963574817	37.0	33.0	37.0	27.0	37.0
26	33.917488526511086	37.0	33.0	37.0	27.0	37.0
27	33.94150074122733	37.0	33.0	37.0	27.0	37.0
28	33.94417271040648	37.0	33.0	37.0	27.0	37.0
29	33.94206886756442	37.0	33.0	37.0	27.0	37.0
30	33.96363695543463	37.0	33.0	37.0	27.0	37.0
31	33.95309998727634	37.0	33.0	37.0	27.0	37.0
32	33.92268155631108	37.0	33.0	37.0	27.0	37.0
33	33.90510810674857	37.0	33.0	37.0	27.0	37.0
34	33.86129432199152	37.0	33.0	37.0	27.0	37.0
35	33.78630164549509	37.0	33.0	37.0	27.0	37.0
36	33.82636638822558	37.0	33.0	37.0	27.0	37.0
37	33.85584385994502	37.0	33.0	37.0	27.0	37.0
38	33.827976079514016	37.0	33.0	37.0	27.0	37.0
39	33.72143759635215	37.0	33.0	37.0	27.0	37.0
40	33.51299145147402	37.0	33.0	37.0	27.0	37.0
41	33.7167328001231	37.0	33.0	37.0	27.0	37.0
42	33.7230709595713	37.0	33.0	37.0	27.0	37.0
43	33.26424088556693	37.0	33.0	37.0	14.0	37.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
14	2.0
15	20.0
16	25.0
17	27.0
18	34.0
19	72.0
20	194.0
21	417.0
22	881.0
23	1724.0
24	2856.0
25	4236.0
26	6148.0
27	8067.0
28	10342.0
29	13044.0
30	15901.0
31	19624.0
32	23840.0
33	30601.0
34	41987.0
35	67502.0
36	90409.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	39.53626687734685	22.618233896429384	13.829141922101593	24.01635730412217
2	15.907833337771821	23.467760309865575	37.85703929244599	22.767367059916616
3	18.009308986752597	28.479995739052473	30.229055519554493	23.281639754640437
4	13.000624347172534	19.79506025985862	39.21876710666868	27.985548286300165
5	14.415909904631711	37.36998931804126	35.47238817231982	12.741712605007205
6	28.343290339189174	41.2418294851651	17.579367545191197	12.835512630454533
7	25.537870650652607	33.12087775519081	24.026121975540978	17.315129618615607
8	23.69974523084571	37.0347355993289	20.901131222388912	18.364387947436477
9	25.37068763999728	15.942157637304597	20.901723020656718	37.78543170204141
10	14.430408962192967	29.142513899861815	34.376969578610044	22.050107559335174
11	32.977366675247744	24.29272709518779	24.850792861729293	17.879113367835174
12	21.97672457412717	26.62766716081822	31.250795228922364	20.14481303613224
13	27.228934200909592	23.138720472965176	26.5525087808068	23.079836545318432
14	21.172174829044273	21.70952765621255	28.800454501069677	28.3178430136735
15	22.889869301352554	29.812429539018737	25.186342479575565	22.111358680053144
16	21.919616041283845	29.452024393924596	26.023737028521715	22.604622536269837
17	21.59915727926664	28.587407124659347	27.261187206505046	22.552248389568962
18	21.991815429956237	28.054196885365716	28.740978775155124	21.21300890952292
19	23.487289652703186	27.408249076054954	28.37376794998121	20.73069332126065
20	23.152035933990824	27.32539731856205	28.530298591816024	20.99226815563111
21	22.12644953588221	27.92577666125171	28.545389447645086	21.402384355220995
22	21.87315987726104	27.810671898163353	28.427325693217696	21.88884253135791
23	21.775217263939066	27.636387308294346	28.750743446573935	21.83765198119265
24	21.963113213967624	28.065441052454037	27.777235296032288	22.19421043754605
25	22.1530804579335	27.88523847990697	28.390930099747596	21.570750962411932
26	22.14331578651469	27.854464969981034	28.306598846585175	21.6956203969191
27	22.164028725887917	27.802386722414063	28.130834761046653	21.902749790651363
28	21.57489355028658	27.916899687234615	28.67558506656251	21.832621695916295
29	22.786896402754227	27.74083970256219	27.89086056345113	21.581403331232448
30	22.248359979050342	27.37481247392389	28.691563619793286	21.685263927232484
31	21.989744136018913	27.68313937145106	28.258959086026753	22.06815740650327
32	21.347347116314992	27.628989829946764	28.59036611599838	22.433296937739865
33	21.303258145363408	27.72782014067045	28.969412906528426	21.99950880743772
34	21.984122052474753	27.497906513627633	28.719674037514093	21.79829739638352
35	21.969622994913493	28.014250502288778	28.116631602619297	21.899494900178425
36	22.45667296931822	27.783449177844254	28.048278902687652	21.711598950149874
37	22.202791512429243	27.49405982488689	28.129355265377136	22.173793397306728
38	21.979979464600106	27.670119809559317	28.13379375238569	22.216106973454888
39	21.690590111642745	27.727228342402643	28.2906202933544	22.291561252600214
40	21.878781960805203	28.034371643394202	28.47822034424905	21.608626051551546
41	21.971398389716914	27.468020701103406	28.373472050847308	22.187108858332373
42	21.781727044884942	27.895890848727483	28.50514716543425	21.817234940953327
43	22.343639500167185	27.25852411429992	28.118406997422717	22.27942938811018
>>END_MODULE
>>Per sequence GC content	pass
#GC Content	Count
0	5.0
1	18.5
2	32.0
3	75.5
4	119.0
5	119.0
6	170.5
7	222.0
8	228.0
9	234.0
10	302.0
11	370.0
12	370.0
13	599.5
14	829.0
15	1560.0
16	2291.0
17	2630.5
18	2970.0
19	2970.0
20	3034.0
21	3098.0
22	3560.5
23	4023.0
24	5211.5
25	6400.0
26	6400.0
27	7860.5
28	9321.0
29	11036.5
30	12752.0
31	14540.5
32	16329.0
33	16329.0
34	18556.0
35	20783.0
36	22131.5
37	23480.0
38	24472.0
39	25464.0
40	25464.0
41	26132.5
42	26801.0
43	27096.0
44	27391.0
45	26285.0
46	25179.0
47	25179.0
48	24382.5
49	23586.0
50	23075.0
51	22564.0
52	21364.5
53	20165.0
54	20165.0
55	18968.5
56	17772.0
57	15330.0
58	12888.0
59	11818.5
60	10749.0
61	10749.0
62	9351.0
63	7953.0
64	6553.5
65	5154.0
66	4292.5
67	3431.0
68	3431.0
69	2791.0
70	2151.0
71	1796.0
72	1441.0
73	1127.5
74	814.0
75	814.0
76	631.0
77	448.0
78	357.0
79	266.0
80	206.0
81	146.0
82	146.0
83	147.5
84	149.0
85	121.5
86	94.0
87	75.0
88	56.0
89	56.0
90	42.5
91	29.0
92	16.5
93	4.0
94	2.0
95	0.0
96	0.0
97	0.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
43	337953.0
>>END_MODULE
>>Sequence Duplication Levels	pass
#Total Deduplicated Percentage	71.55102042822689
#Duplication Level	Percentage of deduplicated	Percentage of total
1	90.40346044118456	64.68459844809598
2	5.572023121596411	7.973678803997948
3	1.437064751266599	3.084703481236837
4	0.6997981899030294	2.002850983255515
5	0.39508186554443897	1.4134255316196072
6	0.2723057161927163	1.16902511172168
7	0.18782378482720413	0.9407288425554722
8	0.1320899055392253	0.7560934023679738
9	0.10989465142488208	0.7076767004149151
>10	0.6928097003165576	9.69370133320981
>50	0.06718507446544486	3.231247975024159
>100	0.02921090194144062	3.4229372376289326
>500	4.172985991634375E-4	0.18780770433257143
>1k	8.34597198326875E-4	0.7315244445386327
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT	1360	0.4024228221083997	No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT	1090	0.3225300559545262	No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT	629	0.18612055522513485	No Hit
GTACATGCTAAGACTTCACCAGTCAAAGCGAACTACTATACTC	367	0.10859498214248726	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	0.0	0.0
3	0.0	0.0	0.0	2.958991339032351E-4	0.0
4	0.0	0.0	0.0	8.876974017097052E-4	0.0
5	0.0	0.0	0.0	8.876974017097052E-4	0.0
6	0.0	0.0	0.0	8.876974017097052E-4	0.0
7	0.0	0.0	0.0011835965356129404	8.876974017097052E-4	0.0
8	0.0	0.0	0.0011835965356129404	8.876974017097052E-4	0.0
9	0.0	0.0	0.0029589913390323506	0.0014794956695161753	0.0
10	0.0	0.0	0.003550789606838821	0.0014794956695161753	0.0
11	0.0	0.0	0.005030285276354996	0.0014794956695161753	0.0
12	0.0	0.0	0.005326184410258231	0.0014794956695161753	0.0
13	0.0	0.0	0.005326184410258231	0.0017753948034194104	0.0
14	0.0	0.0	0.005622083544161466	0.0017753948034194104	0.0
15	0.0	0.0	0.006805680079774406	0.0020712939373226454	0.0
16	0.0	0.0	0.007397478347580876	0.0020712939373226454	0.0
17	0.0	0.0	0.007397478347580876	0.0023671930712258807	0.0
18	0.0	0.0	0.007397478347580876	0.0026630922051291157	0.0
19	0.0	0.0	0.007397478347580876	0.0026630922051291157	0.0
20	0.0	0.0	0.007693377481484112	0.0026630922051291157	0.0
21	0.0	0.0	0.007989276615387347	0.0026630922051291157	0.0
22	0.0	0.0	0.008285175749290581	0.0026630922051291157	0.0
23	0.0	0.0	0.008285175749290581	0.0026630922051291157	0.0
24	0.0	0.0	0.008285175749290581	0.003550789606838821	0.0
25	0.0	0.0	0.008285175749290581	0.003550789606838821	0.0
26	0.0	0.0	0.008285175749290581	0.004142587874645291	0.0
27	0.0	0.0	0.008581074883193818	0.004438487008548526	0.0
28	0.0	0.0	0.008581074883193818	0.009172873151000287	0.0
29	0.0	0.0	0.008581074883193818	0.02219243504274263	0.0
30	0.0	0.0	0.008581074883193818	0.03787508913961409	0.0
31	0.0	0.0	0.008581074883193818	0.0733829852080023	0.0
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
CCGTCCG	25	0.005490922	29.6	28
CGTCCGC	25	0.005490922	29.6	29
GGTATCA	340	0.0	27.205883	1
TGAGTAC	35	8.8563905E-4	26.42857	3
GGCGGAA	35	8.8563905E-4	26.42857	26
CGAGACT	40	0.0019283828	23.125	4
GCGGAAT	40	0.0019283828	23.125	27
CGGAATA	45	0.0038203038	20.555557	28
TCCGCCG	45	0.0038203038	20.555557	31
CGCCCGA	45	0.0038203038	20.555557	10
AGGTCGC	90	9.4287316E-8	20.555557	35
GTACTAA	55	5.132775E-4	20.181818	1
GTATCAA	800	0.0	20.118752	1
GTCGCCC	95	1.6673766E-7	19.473684	37
GGTCGCC	95	1.6673766E-7	19.473684	36
GCTCCGA	95	1.6673766E-7	19.473684	29
CGAACTA	125	4.0927262E-10	19.24	29
GCGAACT	135	5.4569682E-11	19.185184	28
TTAGGCC	50	0.0070248595	18.5	5
TTGGCGG	50	0.0070248595	18.5	24
>>END_MODULE