FastQCFastQC Report
Fri 10 Feb 2017
ERR1633284.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1633284.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences882210
Sequences flagged as poor quality0
Sequence length43
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT38750.4392378231940241No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT27390.31047029618798244No Hit
GCTTTATTGAGCCTGTGTGGGAGCAGGGAGCAAGCTTTGGCCA16000.1813627140930164No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT15600.17682864624069097No Hit
GTATTAGAGGCACCGCCTGCCCAGTGACACATGTTTAACGGCC10530.11935933621246642No Hit
GTGTGGGAGCAGGGAGCAAGCTTTGGCCAGAGCCAAGGGTGCA9610.10893098015211798No Hit
GTACATGCTAAGACTTCACCAGTCAAAGCGAACTACTATACTC9240.10473696738871696No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA8500.023.7235281
GTATCAA19950.021.5137841
TTGCGGT1001.288754E-820.3535
CCGGGTC555.141793E-420.18181814
GCGGATC555.141793E-420.18181835
GCGGATA555.141793E-420.18181819
GGCACCG3200.019.656259
TGCGGTA951.6750164E-719.47368436
TATTAGA3800.019.4736842
GTATTAG4300.019.3604641
GCACCGC3250.019.35384610
CACCGCC3500.019.0285711
TTAACGG3450.017.69565235
ATTAGAG4050.017.3580253
ACCGCCT3750.017.26666612
AACGATT752.0665802E-417.26666622
TAACGGC3550.017.19718436
AACGGCC3450.017.1594237
AGGCACC3900.017.0769238
GTCGCCC2400.016.95833237
Produced by FastQC (version 0.11.5)