FastQCFastQC Report
Fri 10 Feb 2017
ERR1633283.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1633283.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences136590
Sequences flagged as poor quality0
Sequence length43
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT21331.561607731166264No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT20481.4993776996851893No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT14311.047660882934329No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT5530.4048612636356981No Hit
ATCATTAACTGAATCCATAGGTTAATGAGGCGAACCGGGGGAA4300.3148107474924958No Hit
ATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTT3760.2752763745515777No Hit
ACCATGAAGTGCGATTGCCTCGGTGATAACGACATTGGGGCCA3260.2386704736803573No Hit
GATAGAAACCGACCTGGATTACTCCGGTCTGAACTCAGATCAC3130.22915293945383994No Hit
GTATGAAACTGCTCGCCGGCAGCTTCGCCGCTCTCTTCCTGAG2910.21304634307050296No Hit
ACTATACTCAATTGATCCAATAACTTGACCAACGGAACAAGTT2600.1903506845303463No Hit
GTTAATGATAGTGTGTCGAAACACACTGGGTTTCCCCATTCGG2520.18449374039095104No Hit
CCGTATTACCGCGGCTGCTGGCACGGAGTTAGCCGGTGCTTCT2500.1830295043561022No Hit
GTTAATACCTTTGCTCATTGACGTTACCCGCAGAAGAAGCACC2310.16911926202503844No Hit
GTACATGCTAAGACTTCACCAGTCAAAGCGAACTACTATACTC2310.16911926202503844No Hit
ATACAGGGTGACAGCCCCGTACACAAAAATGCACATGCTGTGA2160.1581374917636723No Hit
CATCTTTAGTGACCATGAAGTGCGATTGCCTCGGTGATAACGA2130.15594113771139909No Hit
TCTTTAGTGACCATGAAGTGCGATTGCCTCGGTGATAACGACA2040.1493520755545794No Hit
GTATGAAACTGCTTGTCCGGCGAGTCGCCGTCCGCCGCCTGGG1970.14422724943260853No Hit
GTACTGGTTCACTATCGGTCAGTCAGGAGTATTTAGCCTTGGA1920.1405666593454865No Hit
AGCATGTACTGCTCGGAGGTTGGGTTCTGCTCCGAGGTCGCCC1900.13910242331063766No Hit
GTATGAATGGCTCCACGAGGGTTCAGCTGTCTCTTACTTTTAA1870.13690606925836446No Hit
GTTAGGTACTGTTTGCATTAATAAATTAAAGCTCCATAGGGTC1870.13690606925836446No Hit
TGCTAAGACTTCACCAGTCAAAGCGAACTACTATACTCAATTG1710.1251921809795739No Hit
AGCGTACACGGTGGATGCCCTGGCAGTCAGAGGCGATGAAGGA1630.11933523684017865No Hit
GTAATACGGAGGGTGCAAGCGTTAATCGGAATTACTGGGCGTA1630.11933523684017865No Hit
CCATAGGGTCTTCTCGTCTTGCTGTGTCATGCCCGCCTCTTCA1560.11421041071820778No Hit
ACAATAGGGTTTACGACCTCGATGTTGGATCAGGACATCCCGA1550.11347829270078337No Hit
ATGATAGTGTGTCGAAACACACTGGGTTTCCCCATTCGGAAAT1550.11347829270078337No Hit
GGATTACTCCGGTCTGAACTCAGATCACGTAGGACTTTAATCG1530.11201405666593454No Hit
GAGTTAGCCGGTGCTTCTTCTGCGGGTAACGTCAATGAGCAAA1520.11128193864851015No Hit
ATTCTAGAGTCCATATCAACAATAGGGTTTACGACCTCGATGT1490.1090855845962369No Hit
CCGTCATCTTTAGTGACCATGAAGTGCGATTGCCTCGGTGATA1480.10835346657881251No Hit
GTGGATGCCCTGGCAGTCAGAGGCGATGAAGGACGTGCTAATC1450.10615711252653928No Hit
GTCATCTTTAGTGGCCCCAATGTCGTTATCACCGAGGCAATCG1430.10469287649169047No Hit
ATCCTATTCTAGAGTCCATATCAACAATAGGGTTTACGACCTC1420.10396075847426604No Hit
TCATTAACCTATGGATTCAGTTAATGATAGTGTGTCGAAACAC1410.10322864045684164No Hit
ATACTCAATTGATCCAATAACTTGACCAACGGAACAAGTTACC1390.10176440442199283No Hit
GGTATTAACTTTACTCCCTTCCTCCCCGCTGAAAGTACTTTAC1380.1010322864045684No Hit
CTACTATACTCAATTGATCCAATAACTTGACCAACGGAACAAG1370.100300168387144No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCACCTT250.005479607729.5999985
TCCACTA250.005479607729.59999830
TTACCGA250.005479607729.59999810
CTTACCG250.005479607729.5999989
CTCTTCC900.028.77777733
CCGCTCT900.028.77777728
TTAACTA405.9024595E-527.75000237
AAGGACT405.9024595E-527.7500024
GGCAGCT950.027.26315918
TCTCTTC950.027.26315932
CGCTCTC950.027.26315929
GCAGCTT950.027.26315919
CTCTCTT950.027.26315931
CCGGCAG1050.026.4285716
CGGCAGC1000.025.89999817
GCCGCTC1000.025.89999827
GCTCTCT1000.025.89999830
TTCGCCG1000.025.89999824
GGTATCA5250.025.723811
TGTCCGG809.604264E-1025.43750214
Produced by FastQC (version 0.11.5)