##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	ERR1633283.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	136590
Sequences flagged as poor quality	0
Sequence length	43
%GC	45
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	29.665905263928547	33.0	27.0	33.0	14.0	33.0
2	30.354125485028188	33.0	33.0	33.0	14.0	33.0
3	30.17925177538619	33.0	33.0	33.0	14.0	33.0
4	30.488344681162605	33.0	33.0	33.0	27.0	33.0
5	30.646760377772896	33.0	33.0	33.0	27.0	33.0
6	31.052485540669156	37.0	27.0	37.0	14.0	37.0
7	31.442045537740682	37.0	27.0	37.0	14.0	37.0
8	31.641921077677722	37.0	27.0	37.0	14.0	37.0
9	31.19271542572663	37.0	27.0	37.0	14.0	37.0
10	31.13106376747932	37.0	27.0	37.0	14.0	37.0
11	31.310220367523243	37.0	27.0	37.0	14.0	37.0
12	31.467479317666008	37.0	27.0	37.0	14.0	37.0
13	31.541825902335457	37.0	27.0	37.0	14.0	37.0
14	31.539614905922836	37.0	27.0	37.0	14.0	37.0
15	31.739893110769454	37.0	27.0	37.0	14.0	37.0
16	31.79396002635625	37.0	27.0	37.0	14.0	37.0
17	31.89258364448349	37.0	27.0	37.0	14.0	37.0
18	31.83908778095029	37.0	27.0	37.0	14.0	37.0
19	31.780950289186617	37.0	27.0	37.0	14.0	37.0
20	31.765217072992165	37.0	27.0	37.0	14.0	37.0
21	31.853986382604877	37.0	27.0	37.0	14.0	37.0
22	31.696829928984553	37.0	27.0	37.0	14.0	37.0
23	31.676689362325206	37.0	27.0	37.0	14.0	37.0
24	31.689054835639507	37.0	27.0	37.0	14.0	37.0
25	31.668474998169707	37.0	27.0	37.0	14.0	37.0
26	31.548188007906873	37.0	27.0	37.0	14.0	37.0
27	31.570510286258145	37.0	27.0	37.0	14.0	37.0
28	31.498945750054908	37.0	27.0	37.0	14.0	37.0
29	31.559696903140786	37.0	27.0	37.0	14.0	37.0
30	31.511047660882934	37.0	27.0	37.0	14.0	37.0
31	31.515359836005565	37.0	27.0	37.0	14.0	37.0
32	31.350603997364374	37.0	27.0	37.0	14.0	37.0
33	31.29656636649828	37.0	27.0	37.0	14.0	37.0
34	31.261695585328354	37.0	27.0	37.0	14.0	37.0
35	31.147258218024746	37.0	27.0	37.0	14.0	37.0
36	31.189552675891353	37.0	27.0	37.0	14.0	37.0
37	31.161556482905045	37.0	27.0	37.0	14.0	37.0
38	31.0871806135149	37.0	27.0	37.0	14.0	37.0
39	30.946760377772897	37.0	27.0	37.0	14.0	37.0
40	30.70105424994509	37.0	27.0	37.0	14.0	37.0
41	30.833194230910024	37.0	27.0	37.0	14.0	37.0
42	30.736906069258364	37.0	27.0	37.0	14.0	37.0
43	30.184779266417745	37.0	27.0	37.0	14.0	37.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
14	3.0
15	9.0
16	25.0
17	37.0
18	82.0
19	198.0
20	519.0
21	999.0
22	1627.0
23	2621.0
24	3733.0
25	5004.0
26	6331.0
27	7657.0
28	8814.0
29	9948.0
30	11165.0
31	11912.0
32	12423.0
33	13170.0
34	13044.0
35	13233.0
36	14036.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	44.118895966029726	22.21685335676111	12.766673987846842	20.897576689362324
2	18.048905483563953	26.584669448715132	33.74185518705615	21.62456988066476
3	21.074749249579032	28.87619884325353	29.376235449154404	20.67281645801303
4	13.851672889669814	22.57193059521195	35.73248407643312	27.843912438685116
5	17.17988139688118	36.994655538472806	31.94523757229665	13.880225492349366
6	27.210630353613002	40.53664250677209	15.829855772750568	16.42287136686434
7	26.179076067062013	34.15769822095322	20.95321765868658	18.710008053298193
8	26.21421773189838	34.00175708324182	18.6712057983747	21.1128193864851
9	27.70114942528736	13.432169265685628	19.67420748224614	39.192473826780876
10	18.190936378944286	27.765575810820703	30.283329672743246	23.760158137491764
11	34.870049051907166	23.450472216121238	21.56600043927081	20.113478292700783
12	23.660590087122042	28.898894501793688	27.37608902555092	20.06442638553335
13	31.498645581667766	22.338384947653562	24.87370964199429	21.289259828684383
14	22.351563071967202	22.49945091148693	29.774507650633282	25.374478365912584
15	27.197452229299362	26.37894428581887	26.477048100153745	19.94655538472802
16	21.164067647704808	27.368035727359253	27.139614905922834	24.328281719013106
17	22.664177465407423	26.766234717036387	25.693681821509628	24.875905996046562
18	23.528076725968226	23.728677062742516	29.718134563291603	23.025111647997658
19	26.813090270151545	23.76747931766601	30.578373233765284	18.841057178417163
20	25.532615857676255	22.871366864338533	31.17358518193133	20.422432096053882
21	23.06391390292115	26.173219122922614	30.58642653195695	20.176440442199283
22	22.821582839153674	26.045830587890766	29.990482465773482	21.142104107182078
23	22.150962735192913	25.25075042096786	31.762940185957977	20.835346657881253
24	22.350098835932354	25.915513580789224	29.9699831612856	21.764404421992825
25	22.271030090050516	25.90672816458013	31.6736217878322	20.148619957537157
26	22.33472435756644	26.600776045098467	30.727725309319865	20.336774288015228
27	23.179588549674207	25.18412768138224	30.953217658686583	20.683066110256974
28	21.4876638114064	26.28742953364082	30.715279303023646	21.50962735192913
29	22.84793908778095	25.758108207042973	29.65004758767113	21.74390511750494
30	22.95922102642946	24.782927007833663	31.235083095394977	21.0227688703419
31	22.91090123727945	25.272713961490595	30.34922029431144	21.467164506918515
32	20.356541474485688	25.71344900798009	30.879273738926717	23.05073577860751
33	20.50662566805769	26.201771725602168	31.371989164653343	21.9196134416868
34	21.334651145764695	25.9777436122703	31.076945603631305	21.610659638333697
35	21.59601727798521	26.54074236766967	30.39607584742661	21.467164506918515
36	22.50603997364375	26.3796764038363	29.808917197452228	21.30536642506772
37	22.65319569514606	26.325499670546893	29.538033531005198	21.483271103301853
38	22.192693462186107	26.704736803572736	28.976499011640676	22.12607072260048
39	21.99428947946409	26.22227103009005	29.255435976279376	22.528003514166485
40	22.016985138004248	27.633062449666884	29.629548283183247	20.720404129145617
41	22.616589794274837	25.888425214144522	28.772238084779268	22.722746906801376
42	20.650120799472877	28.14993776996852	28.984552309832345	22.21538912072626
43	22.24174536935354	26.812358152134124	28.200453913170804	22.745442565341534
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	2.0
1	6.0
2	10.0
3	63.0
4	116.0
5	116.0
6	180.0
7	244.0
8	313.0
9	382.0
10	483.0
11	584.0
12	584.0
13	1068.0
14	1552.0
15	2757.5
16	3963.0
17	3875.5
18	3788.0
19	3788.0
20	3332.5
21	2877.0
22	2091.0
23	1305.0
24	1233.5
25	1162.0
26	1162.0
27	1288.5
28	1415.0
29	1529.0
30	1643.0
31	2075.5
32	2508.0
33	2508.0
34	3696.5
35	4885.0
36	5347.5
37	5810.0
38	6523.5
39	7237.0
40	7237.0
41	7950.0
42	8663.0
43	9423.0
44	10183.0
45	10477.5
46	10772.0
47	10772.0
48	11292.0
49	11812.0
50	12470.5
51	13129.0
52	12531.0
53	11933.0
54	11933.0
55	10858.0
56	9783.0
57	8222.0
58	6661.0
59	5990.0
60	5319.0
61	5319.0
62	4420.0
63	3521.0
64	2614.0
65	1707.0
66	1506.5
67	1306.0
68	1306.0
69	1080.5
70	855.0
71	719.5
72	584.0
73	495.0
74	406.0
75	406.0
76	283.0
77	160.0
78	126.0
79	92.0
80	81.0
81	70.0
82	70.0
83	61.5
84	53.0
85	50.5
86	48.0
87	41.0
88	34.0
89	34.0
90	24.5
91	15.0
92	8.0
93	1.0
94	0.5
95	0.0
96	0.0
97	0.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
43	136590.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	43.69939234204554
#Duplication Level	Percentage of deduplicated	Percentage of total
1	82.56630199869322	36.080972252727136
2	7.250917254435491	6.337213558825683
3	2.7810819413962373	3.645947726773556
4	1.5312704183350365	2.6766234717036386
5	1.023639196501868	2.236620543231569
6	0.7539077551977752	1.976718647045904
7	0.5511903365779289	1.6860677941284135
8	0.40208413610548005	1.4056665934548649
9	0.2931863492435792	1.1530858774434438
>10	2.439310425706579	21.189691778314664
>50	0.2697314413040929	8.323449740098104
>100	0.130677344234281	8.77443443883154
>500	0.001675350567106167	0.4048612636356981
>1k	0.005026051701318501	4.1086463137857825
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	fail
#Sequence	Count	Percentage	Possible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT	2133	1.561607731166264	No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT	2048	1.4993776996851893	No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT	1431	1.047660882934329	No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	553	0.4048612636356981	No Hit
ATCATTAACTGAATCCATAGGTTAATGAGGCGAACCGGGGGAA	430	0.3148107474924958	No Hit
ATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTT	376	0.2752763745515777	No Hit
ACCATGAAGTGCGATTGCCTCGGTGATAACGACATTGGGGCCA	326	0.2386704736803573	No Hit
GATAGAAACCGACCTGGATTACTCCGGTCTGAACTCAGATCAC	313	0.22915293945383994	No Hit
GTATGAAACTGCTCGCCGGCAGCTTCGCCGCTCTCTTCCTGAG	291	0.21304634307050296	No Hit
ACTATACTCAATTGATCCAATAACTTGACCAACGGAACAAGTT	260	0.1903506845303463	No Hit
GTTAATGATAGTGTGTCGAAACACACTGGGTTTCCCCATTCGG	252	0.18449374039095104	No Hit
CCGTATTACCGCGGCTGCTGGCACGGAGTTAGCCGGTGCTTCT	250	0.1830295043561022	No Hit
GTTAATACCTTTGCTCATTGACGTTACCCGCAGAAGAAGCACC	231	0.16911926202503844	No Hit
GTACATGCTAAGACTTCACCAGTCAAAGCGAACTACTATACTC	231	0.16911926202503844	No Hit
ATACAGGGTGACAGCCCCGTACACAAAAATGCACATGCTGTGA	216	0.1581374917636723	No Hit
CATCTTTAGTGACCATGAAGTGCGATTGCCTCGGTGATAACGA	213	0.15594113771139909	No Hit
TCTTTAGTGACCATGAAGTGCGATTGCCTCGGTGATAACGACA	204	0.1493520755545794	No Hit
GTATGAAACTGCTTGTCCGGCGAGTCGCCGTCCGCCGCCTGGG	197	0.14422724943260853	No Hit
GTACTGGTTCACTATCGGTCAGTCAGGAGTATTTAGCCTTGGA	192	0.1405666593454865	No Hit
AGCATGTACTGCTCGGAGGTTGGGTTCTGCTCCGAGGTCGCCC	190	0.13910242331063766	No Hit
GTATGAATGGCTCCACGAGGGTTCAGCTGTCTCTTACTTTTAA	187	0.13690606925836446	No Hit
GTTAGGTACTGTTTGCATTAATAAATTAAAGCTCCATAGGGTC	187	0.13690606925836446	No Hit
TGCTAAGACTTCACCAGTCAAAGCGAACTACTATACTCAATTG	171	0.1251921809795739	No Hit
AGCGTACACGGTGGATGCCCTGGCAGTCAGAGGCGATGAAGGA	163	0.11933523684017865	No Hit
GTAATACGGAGGGTGCAAGCGTTAATCGGAATTACTGGGCGTA	163	0.11933523684017865	No Hit
CCATAGGGTCTTCTCGTCTTGCTGTGTCATGCCCGCCTCTTCA	156	0.11421041071820778	No Hit
ACAATAGGGTTTACGACCTCGATGTTGGATCAGGACATCCCGA	155	0.11347829270078337	No Hit
ATGATAGTGTGTCGAAACACACTGGGTTTCCCCATTCGGAAAT	155	0.11347829270078337	No Hit
GGATTACTCCGGTCTGAACTCAGATCACGTAGGACTTTAATCG	153	0.11201405666593454	No Hit
GAGTTAGCCGGTGCTTCTTCTGCGGGTAACGTCAATGAGCAAA	152	0.11128193864851015	No Hit
ATTCTAGAGTCCATATCAACAATAGGGTTTACGACCTCGATGT	149	0.1090855845962369	No Hit
CCGTCATCTTTAGTGACCATGAAGTGCGATTGCCTCGGTGATA	148	0.10835346657881251	No Hit
GTGGATGCCCTGGCAGTCAGAGGCGATGAAGGACGTGCTAATC	145	0.10615711252653928	No Hit
GTCATCTTTAGTGGCCCCAATGTCGTTATCACCGAGGCAATCG	143	0.10469287649169047	No Hit
ATCCTATTCTAGAGTCCATATCAACAATAGGGTTTACGACCTC	142	0.10396075847426604	No Hit
TCATTAACCTATGGATTCAGTTAATGATAGTGTGTCGAAACAC	141	0.10322864045684164	No Hit
ATACTCAATTGATCCAATAACTTGACCAACGGAACAAGTTACC	139	0.10176440442199283	No Hit
GGTATTAACTTTACTCCCTTCCTCCCCGCTGAAAGTACTTTAC	138	0.1010322864045684	No Hit
CTACTATACTCAATTGATCCAATAACTTGACCAACGGAACAAG	137	0.100300168387144	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0014642360348488177	0.0	0.0
2	0.0	0.0	0.0014642360348488177	0.0	0.0
3	0.0	0.0	0.0014642360348488177	0.0	0.0
4	0.0	0.0	0.0029284720696976354	0.0	0.0
5	7.321180174244088E-4	0.0	0.005856944139395271	0.0	0.0
6	7.321180174244088E-4	0.0	0.010981770261366132	0.0	0.0
7	7.321180174244088E-4	0.0	0.022695658540156673	7.321180174244088E-4	0.0
8	7.321180174244088E-4	0.0	0.028552602679551942	7.321180174244088E-4	0.0
9	7.321180174244088E-4	0.0	0.03148107474924958	0.0021963540522732263	0.0
10	0.0021963540522732263	0.0	0.03514166483637162	0.0021963540522732263	0.0
11	0.0021963540522732263	0.0	0.05344461527198184	0.0021963540522732263	0.0
12	0.0021963540522732263	0.0	0.057105205359103885	0.0029284720696976354	0.0
13	0.0021963540522732263	0.0	0.06003367742880152	0.0029284720696976354	0.0
14	0.0021963540522732263	0.0	0.06515850355077238	0.003660590087122044	0.0
15	0.0021963540522732263	0.0	0.06955121165531884	0.003660590087122044	0.0
16	0.0021963540522732263	0.0	0.07760450984698733	0.004392708104546453	0.0
17	0.0021963540522732263	0.0	0.07833662786441174	0.005856944139395271	0.0
18	0.0021963540522732263	0.0	0.07980086389926055	0.005856944139395271	0.0
19	0.0021963540522732263	0.0	0.07980086389926055	0.005856944139395271	0.0
20	0.0021963540522732263	0.0	0.07980086389926055	0.005856944139395271	0.0
21	0.0021963540522732263	0.0	0.0819972179515338	0.005856944139395271	0.0
22	0.0021963540522732263	0.0	0.0819972179515338	0.008053298191668497	0.0
23	0.0021963540522732263	0.0	0.08272933596895819	0.010981770261366132	0.0
24	0.0021963540522732263	0.0	0.08272933596895819	0.013178124313639359	0.0
25	0.0021963540522732263	0.0	0.0834614539863826	0.013910242331063768	0.0
26	0.0021963540522732263	0.0	0.0834614539863826	0.016106596383336995	0.0
27	0.0021963540522732263	0.0	0.0834614539863826	0.016838714400761404	0.0
28	0.0021963540522732263	0.0	0.0834614539863826	0.03514166483637162	0.0
29	0.0021963540522732263	0.0	0.0834614539863826	0.07028332967274324	0.0
30	0.0021963540522732263	0.0	0.0834614539863826	0.12079947287502746	0.0
31	0.0021963540522732263	0.0	0.0834614539863826	0.19693974668716596	0.0
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
TCACCTT	25	0.0054796077	29.599998	5
TCCACTA	25	0.0054796077	29.599998	30
TTACCGA	25	0.0054796077	29.599998	10
CTTACCG	25	0.0054796077	29.599998	9
CTCTTCC	90	0.0	28.777777	33
CCGCTCT	90	0.0	28.777777	28
TTAACTA	40	5.9024595E-5	27.750002	37
AAGGACT	40	5.9024595E-5	27.750002	4
GGCAGCT	95	0.0	27.263159	18
TCTCTTC	95	0.0	27.263159	32
CGCTCTC	95	0.0	27.263159	29
GCAGCTT	95	0.0	27.263159	19
CTCTCTT	95	0.0	27.263159	31
CCGGCAG	105	0.0	26.42857	16
CGGCAGC	100	0.0	25.899998	17
GCCGCTC	100	0.0	25.899998	27
GCTCTCT	100	0.0	25.899998	30
TTCGCCG	100	0.0	25.899998	24
GGTATCA	525	0.0	25.72381	1
TGTCCGG	80	9.604264E-10	25.437502	14
>>END_MODULE