FastQCFastQC Report
Fri 10 Feb 2017
ERR1633282.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1633282.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences682737
Sequences flagged as poor quality0
Sequence length43
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT21100.30905019062977396No Hit
GTATTAGAGGCACCGCCTGCCCAGTGACACATGTTTAACGGCC16260.2381590568549822No Hit
TTATTACCCTCACTGTCAACCCAACACAGGCATGCTCATAAGG15980.23405791688453975No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT14940.218825111280039No Hit
GTACATGCTAAGACTTCACCAGTCAAAGCGAACTACTATACTC14280.20915813849256742No Hit
AGCATGTACTGCTCGGAGGTTGGGTTCTGCTCCGAGGTCGCCC13410.19641531072726393No Hit
GTATGAATGGCTCCACGAGGGTTCAGCTGTCTCTTACTTTTAA12540.18367248296196045No Hit
CCCCTATACCTTCTGCATAATGAATTAACTAGAAATAACTTTG12410.18176838226139788No Hit
GTACCCTAACCGTGCAAAGGTAGCATAATCACTTGTTCCTTAA10270.1504239553444445No Hit
CTCTAATACTGGTGATGCTAGAGGTGATGTTTTTGGTAAACAG9810.1436863682501461No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT9570.14017110541833824No Hit
TGCTAAGACTTCACCAGTCAAAGCGAACTACTATACTCAATTG9160.13416586474733316No Hit
GATAGAAACCGACCTGGATTACTCCGGTCTGAACTCAGATCAC8770.1284535626456454No Hit
CCCTATAGAAGAACTAATGTTAGTATAAGTAACATGAAAACAT8720.12772121622235208No Hit
GGATTACTCCGGTCTGAACTCAGATCACGTAGGACTTTAATCG8700.12742827765303477No Hit
GTTAGGGTACCGCGGCCGTTAAACATGTGTCACTGGGCAGGCG8690.1272818083683761No Hit
CCATAGGGTCTTCTCGTCTTGCTGTGTCATGCCCGCCTCTTCA8650.12669593122974146No Hit
CCTTTAATAGCGGCTGCACCATCGGGATGTCCTGATCCAACAT8380.12274126054395763No Hit
ATATAGTACCGCAAGGGAAAGATGAAAAATTATAGCCAAGCAT8330.12200891412066434No Hit
CTACTATACTCAATTGATCCAATAACTTGACCAACGGAACAAG8190.11995834413544308No Hit
ACTATACTCAATTGATCCAATAACTTGACCAACGGAACAAGTT8150.11937246699680844No Hit
GTTAGGTACTGTTTGCATTAATAAATTAAAGCTCCATAGGGTC8150.11937246699680844No Hit
ATCTATCACCCTATAGAAGAACTAATGTTAGTATAAGTAACAT8070.11820071271953915No Hit
CTCCAGGACAGGCTGCATCAGAAGAGGCCATCAAGCAGATCAC8000.11717542772692853No Hit
GCATAATATAGCAAGGACTAACCCCTATACCTTCTGCATAATG7830.11468544988773129No Hit
GAATAGGATTGCGCTGTTATCCCTAGGGTAACTTGTTCCGTTG7780.11395310346443799No Hit
GTACCTAACAAACCCACAGGTCCTAAACTACCAAACCTGCATT7770.11380663417977932No Hit
GTTTAGGACCTGTGGGTTTGTTAGGTACTGTTTGCATTAATAA7560.11073077920194746No Hit
CTAGAATAGGATTGCGCTGTTATCCCTAGGGTAACTTGTTCCG7350.10765492422411557No Hit
GTATAGTAGTTCGCTTTGACTGGTGAAGTCTTAGCATGTACTG7170.10501847710025969No Hit
GTCTTAGCATGTACTGCTCGGAGGTTGGGTTCTGCTCCGAGGT6880.10077086784515853No Hit
TCTATAGGGTGATAGATTGGTCCAATTGGGTGTGAGGAGTTCA6840.10018499070652388No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TGCGGTA509.084029E-625.90000236
GGTATCA5250.025.723811
ATCGCTA400.001930388723.12510
GTTAGCC603.723435E-521.58333228
GTATCAA13700.021.3357681
TTAGCCG656.896883E-519.92307729
CGAACCG902.1496107E-618.531
CACGGTA500.007032027518.52
AGACGAA500.007032027518.530
CGTAGGC500.007032027518.518
CGGTACT500.007032027518.54
CGGTAAT500.007032027518.51
ACGGTAC500.007032027518.53
CTATACA752.0657179E-417.2666664
TAGCCGG752.0657179E-417.26666630
AACCGGG1005.870759E-616.65000233
GCGAACC1005.870759E-616.65000230
GGCAACT1005.870759E-616.65000230
CATGCTA3150.016.4444454
GCTCGGA4200.016.2976211
Produced by FastQC (version 0.11.5)