##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	ERR1633282.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	682737
Sequences flagged as poor quality	0
Sequence length	43
%GC	45
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.758609830725447	33.0	33.0	33.0	27.0	33.0
2	31.900210476362055	33.0	33.0	33.0	33.0	33.0
3	31.615994152946158	33.0	33.0	33.0	27.0	33.0
4	31.91743087015937	33.0	33.0	33.0	33.0	33.0
5	32.01336092814656	33.0	33.0	33.0	33.0	33.0
6	35.15969985514188	37.0	37.0	37.0	33.0	37.0
7	35.311891694752156	37.0	37.0	37.0	33.0	37.0
8	35.43814675343507	37.0	37.0	37.0	33.0	37.0
9	35.550510665160964	37.0	37.0	37.0	33.0	37.0
10	35.550201614970334	37.0	37.0	37.0	33.0	37.0
11	35.56382325844359	37.0	37.0	37.0	33.0	37.0
12	35.56138454485402	37.0	37.0	37.0	33.0	37.0
13	35.567055835556005	37.0	37.0	37.0	33.0	37.0
14	35.533461640426694	37.0	37.0	37.0	33.0	37.0
15	35.54341569301209	37.0	37.0	37.0	33.0	37.0
16	35.531157678579014	37.0	37.0	37.0	33.0	37.0
17	35.53184754890976	37.0	37.0	37.0	33.0	37.0
18	35.51502115748817	37.0	37.0	37.0	33.0	37.0
19	35.51649610318468	37.0	37.0	37.0	33.0	37.0
20	35.508011137524406	37.0	37.0	37.0	33.0	37.0
21	35.52481555855329	37.0	37.0	37.0	33.0	37.0
22	35.42096590634461	37.0	37.0	37.0	33.0	37.0
23	35.47672969239986	37.0	37.0	37.0	33.0	37.0
24	35.45673370565825	37.0	37.0	37.0	33.0	37.0
25	35.47768467213583	37.0	37.0	37.0	33.0	37.0
26	35.3749906625831	37.0	37.0	37.0	33.0	37.0
27	35.38448773100037	37.0	37.0	37.0	33.0	37.0
28	35.401037295473955	37.0	37.0	37.0	33.0	37.0
29	35.41536199151357	37.0	37.0	37.0	33.0	37.0
30	35.41328505705711	37.0	37.0	37.0	33.0	37.0
31	35.392054334245834	37.0	37.0	37.0	33.0	37.0
32	35.382254074409325	37.0	37.0	37.0	33.0	37.0
33	35.38705387286759	37.0	37.0	37.0	33.0	37.0
34	35.37434033895922	37.0	37.0	37.0	33.0	37.0
35	35.31493532648736	37.0	37.0	37.0	33.0	37.0
36	35.34064508002349	37.0	37.0	37.0	33.0	37.0
37	35.34556937737372	37.0	37.0	37.0	33.0	37.0
38	35.33862966266659	37.0	37.0	37.0	33.0	37.0
39	35.23833335530372	37.0	37.0	37.0	33.0	37.0
40	35.0456134646284	37.0	37.0	37.0	27.0	37.0
41	35.217438047154324	37.0	37.0	37.0	33.0	37.0
42	35.26842986391539	37.0	37.0	37.0	33.0	37.0
43	34.930553053371945	37.0	37.0	37.0	27.0	37.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
14	5.0
15	17.0
16	50.0
17	29.0
18	22.0
19	27.0
20	42.0
21	144.0
22	361.0
23	888.0
24	1648.0
25	3184.0
26	4818.0
27	7212.0
28	10074.0
29	13532.0
30	17384.0
31	22078.0
32	28246.0
33	37242.0
34	53024.0
35	108173.0
36	374537.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	40.50080777810489	21.241415069052945	13.548848238780087	24.708928914062074
2	16.158052075689465	21.077808878089222	39.0870862425795	23.677052803641814
3	17.13646689720932	27.726049708745826	29.712173208717267	25.42531018532759
4	12.194007355687475	18.088663716775276	40.280957381832245	29.436371545705008
5	13.157335840887487	37.70221915613186	36.31412974542174	12.826315257558912
6	29.302059211673015	40.58619937106089	17.067772802704408	13.043968614561683
7	26.666490903525077	32.07516217811544	24.05772647446967	17.200620443889814
8	24.057140597331035	36.549798824437524	20.38574150807705	19.007319070154395
9	25.478185597089364	15.734755843025939	20.580692125957725	38.20636643392697
10	15.223431570282553	27.8972723025118	34.20336088420578	22.675935242999866
11	33.47643382444485	23.95138977380748	24.21752446403227	18.354651937715403
12	22.89314919214866	26.5983826861588	29.676874111114525	20.831594010578012
13	26.73708909873055	22.5606639159735	26.380729329156026	24.321517656139918
14	21.574632691651395	21.050565591142707	27.858018534223284	29.516783182982614
15	23.97936540717729	28.891945214628766	24.819659693264022	22.30902968492992
16	23.030683850443143	28.528115511536654	25.197696916968027	23.24350372105218
17	22.046410257536944	28.180104491187674	26.244366425138814	23.529118826136568
18	22.027515719815977	28.305335729570828	27.986765035438243	21.68038351517495
19	24.608450984786234	26.512698154633483	27.424908859487623	21.45394200109266
20	23.571009041548944	26.934676163735084	27.861826735624405	21.632488059091568
21	22.689703355757782	27.313885141716355	27.930520830129318	22.065890672396545
22	22.31430257917763	27.40030201966497	27.493602953992536	22.79179244716487
23	22.341838804693463	27.349770116457726	28.186695608997315	22.121695469851495
24	22.822697466227844	28.22521703086254	25.970175924257806	22.98190957865181
25	22.572234989461535	27.645198663614245	27.668487279874977	22.114079067049243
26	22.76747854591153	27.63128408157167	27.91763153307936	21.68360583943744
27	22.715335480573046	27.665264955612486	26.43067535522463	23.188724208589836
28	21.83197922479666	27.220584207388786	28.519327354457136	22.428109213357413
29	24.265859327969626	26.917539257430022	26.97876341841734	21.83783799618301
30	23.214063394835787	26.60497380396844	27.84688686858922	22.334075932606552
31	22.468095328069225	27.30070290609708	27.871640177696538	22.359561588137158
32	21.576097384497984	26.87916430484945	28.085485333298184	23.45925297735438
33	21.677014721627803	27.120399216682266	28.565758117693928	22.636827943996003
34	22.972974952287633	26.84752693936318	28.233858718657405	21.945639389691785
35	23.031416196866434	27.652522127847178	27.11703042313512	22.19903125215127
36	23.88855445068892	27.351820686442952	26.60966082107751	22.149964041790614
37	22.3966183171558	26.586958081955427	27.555705930687807	23.46071767020097
38	22.915705461986093	27.280197206244868	27.336880819407767	22.467216512361276
39	21.932896561926484	27.308612247468645	27.818178888796126	22.94031230180875
40	22.46765592021525	27.97753747050475	27.800602574637086	21.754204034642914
41	22.249563155358505	26.541992011565213	27.854796209960792	23.35364862311549
42	21.923961935562303	27.852745639975566	27.635531690826774	22.587760733635353
43	23.21142694771193	26.15545956935101	27.502098172502738	23.131015310434325
>>END_MODULE
>>Per sequence GC content	pass
#GC Content	Count
0	45.0
1	41.5
2	38.0
3	125.5
4	213.0
5	213.0
6	271.0
7	329.0
8	289.0
9	249.0
10	355.0
11	461.0
12	461.0
13	813.0
14	1165.0
15	1987.0
16	2809.0
17	3333.0
18	3857.0
19	3857.0
20	3923.0
21	3989.0
22	4529.5
23	5070.0
24	6803.5
25	8537.0
26	8537.0
27	11192.5
28	13848.0
29	16478.5
30	19109.0
31	23401.0
32	27693.0
33	27693.0
34	34223.5
35	40754.0
36	44727.5
37	48701.0
38	51834.0
39	54967.0
40	54967.0
41	57540.0
42	60113.0
43	60740.5
44	61368.0
45	58327.0
46	55286.0
47	55286.0
48	51315.5
49	47345.0
50	46844.5
51	46344.0
52	43286.0
53	40228.0
54	40228.0
55	40574.5
56	40921.0
57	33740.0
58	26559.0
59	24293.0
60	22027.0
61	22027.0
62	19870.5
63	17714.0
64	14132.0
65	10550.0
66	8696.5
67	6843.0
68	6843.0
69	5765.0
70	4687.0
71	4082.0
72	3477.0
73	3330.5
74	3184.0
75	3184.0
76	2691.0
77	2198.0
78	1673.5
79	1149.0
80	765.5
81	382.0
82	382.0
83	319.0
84	256.0
85	200.0
86	144.0
87	112.0
88	80.0
89	80.0
90	56.5
91	33.0
92	23.0
93	13.0
94	7.5
95	2.0
96	2.0
97	1.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
43	682737.0
>>END_MODULE
>>Sequence Duplication Levels	warn
#Total Deduplicated Percentage	50.49515508406072
#Duplication Level	Percentage of deduplicated	Percentage of total
1	86.11795650703694	43.48539569345225
2	7.772319874723838	7.849289948742151
3	1.9794367069854661	2.998558904949407
4	0.9288863935910802	1.876170499994218
5	0.5515415826531388	1.3925088875689267
6	0.39936899621335964	1.2099719639735553
7	0.26697010120824693	0.9436487664322469
8	0.19337095617820274	0.7811437136777164
9	0.1746786631383478	0.7938383566542544
>10	1.2805519496389726	13.023800791786977
>50	0.18246865793583622	6.445784283316098
>100	0.1377343973088569	12.924261987974084
>500	0.012066474977912507	4.326019529826174
>1k	0.002648738409785672	1.9496066716519946
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT	2110	0.30905019062977396	No Hit
GTATTAGAGGCACCGCCTGCCCAGTGACACATGTTTAACGGCC	1626	0.2381590568549822	No Hit
TTATTACCCTCACTGTCAACCCAACACAGGCATGCTCATAAGG	1598	0.23405791688453975	No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT	1494	0.218825111280039	No Hit
GTACATGCTAAGACTTCACCAGTCAAAGCGAACTACTATACTC	1428	0.20915813849256742	No Hit
AGCATGTACTGCTCGGAGGTTGGGTTCTGCTCCGAGGTCGCCC	1341	0.19641531072726393	No Hit
GTATGAATGGCTCCACGAGGGTTCAGCTGTCTCTTACTTTTAA	1254	0.18367248296196045	No Hit
CCCCTATACCTTCTGCATAATGAATTAACTAGAAATAACTTTG	1241	0.18176838226139788	No Hit
GTACCCTAACCGTGCAAAGGTAGCATAATCACTTGTTCCTTAA	1027	0.1504239553444445	No Hit
CTCTAATACTGGTGATGCTAGAGGTGATGTTTTTGGTAAACAG	981	0.1436863682501461	No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT	957	0.14017110541833824	No Hit
TGCTAAGACTTCACCAGTCAAAGCGAACTACTATACTCAATTG	916	0.13416586474733316	No Hit
GATAGAAACCGACCTGGATTACTCCGGTCTGAACTCAGATCAC	877	0.1284535626456454	No Hit
CCCTATAGAAGAACTAATGTTAGTATAAGTAACATGAAAACAT	872	0.12772121622235208	No Hit
GGATTACTCCGGTCTGAACTCAGATCACGTAGGACTTTAATCG	870	0.12742827765303477	No Hit
GTTAGGGTACCGCGGCCGTTAAACATGTGTCACTGGGCAGGCG	869	0.1272818083683761	No Hit
CCATAGGGTCTTCTCGTCTTGCTGTGTCATGCCCGCCTCTTCA	865	0.12669593122974146	No Hit
CCTTTAATAGCGGCTGCACCATCGGGATGTCCTGATCCAACAT	838	0.12274126054395763	No Hit
ATATAGTACCGCAAGGGAAAGATGAAAAATTATAGCCAAGCAT	833	0.12200891412066434	No Hit
CTACTATACTCAATTGATCCAATAACTTGACCAACGGAACAAG	819	0.11995834413544308	No Hit
ACTATACTCAATTGATCCAATAACTTGACCAACGGAACAAGTT	815	0.11937246699680844	No Hit
GTTAGGTACTGTTTGCATTAATAAATTAAAGCTCCATAGGGTC	815	0.11937246699680844	No Hit
ATCTATCACCCTATAGAAGAACTAATGTTAGTATAAGTAACAT	807	0.11820071271953915	No Hit
CTCCAGGACAGGCTGCATCAGAAGAGGCCATCAAGCAGATCAC	800	0.11717542772692853	No Hit
GCATAATATAGCAAGGACTAACCCCTATACCTTCTGCATAATG	783	0.11468544988773129	No Hit
GAATAGGATTGCGCTGTTATCCCTAGGGTAACTTGTTCCGTTG	778	0.11395310346443799	No Hit
GTACCTAACAAACCCACAGGTCCTAAACTACCAAACCTGCATT	777	0.11380663417977932	No Hit
GTTTAGGACCTGTGGGTTTGTTAGGTACTGTTTGCATTAATAA	756	0.11073077920194746	No Hit
CTAGAATAGGATTGCGCTGTTATCCCTAGGGTAACTTGTTCCG	735	0.10765492422411557	No Hit
GTATAGTAGTTCGCTTTGACTGGTGAAGTCTTAGCATGTACTG	717	0.10501847710025969	No Hit
GTCTTAGCATGTACTGCTCGGAGGTTGGGTTCTGCTCCGAGGT	688	0.10077086784515853	No Hit
TCTATAGGGTGATAGATTGGTCCAATTGGGTGTGAGGAGTTCA	684	0.10018499070652388	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	1.4646928465866067E-4	0.0	0.0
2	0.0	0.0	1.4646928465866067E-4	0.0	0.0
3	0.0	0.0	1.4646928465866067E-4	0.0	0.0
4	0.0	0.0	1.4646928465866067E-4	1.4646928465866067E-4	0.0
5	0.0	0.0	1.4646928465866067E-4	2.9293856931732133E-4	0.0
6	0.0	0.0	2.9293856931732133E-4	2.9293856931732133E-4	0.0
7	0.0	0.0	4.3940785397598195E-4	2.9293856931732133E-4	0.0
8	0.0	0.0	4.3940785397598195E-4	2.9293856931732133E-4	0.0
9	0.0	0.0	5.858771386346427E-4	2.9293856931732133E-4	0.0
10	0.0	0.0	0.0011717542772692853	2.9293856931732133E-4	0.0
11	0.0	0.0	0.0019041007005625886	2.9293856931732133E-4	0.0
12	0.0	0.0	0.0024899778391972312	4.3940785397598195E-4	0.0
13	0.0	0.0	0.0032223242624905345	4.3940785397598195E-4	0.0
14	0.0	0.0	0.003368793547149195	4.3940785397598195E-4	0.0
15	0.0	0.0	0.003954670685783838	4.3940785397598195E-4	0.0
16	0.0	0.0	0.003954670685783838	5.858771386346427E-4	0.0
17	0.0	0.0	0.004101139970442498	5.858771386346427E-4	0.0
18	0.0	0.0	0.004247609255101159	5.858771386346427E-4	0.0
19	0.0	0.0	0.004247609255101159	5.858771386346427E-4	0.0
20	0.0	0.0	0.004394078539759819	7.323464232933032E-4	0.0
21	0.0	0.0	0.004394078539759819	0.0014646928465866065	0.0
22	0.0	0.0	0.00454054782441848	0.002050569985221249	0.0
23	0.0	0.0	0.00454054782441848	0.0021970392698799097	0.0
24	0.0	0.0	0.004687017109077141	0.0024899778391972312	0.0
25	0.0	0.0	0.004687017109077141	0.002636447123855892	0.0
26	0.0	0.0	0.0048334863937358015	0.003075854977831874	0.0
27	0.0	0.0	0.0048334863937358015	0.0036617321164665166	0.0
28	0.0	0.0	0.0048334863937358015	0.0089346263641783	0.0
29	0.0	0.0	0.0048334863937358015	0.01977335342891919	0.0
30	0.0	0.0	0.0048334863937358015	0.036470851880006504	0.0
31	0.0	0.0	0.0048334863937358015	0.07865400586170077	0.0
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
TGCGGTA	50	9.084029E-6	25.900002	36
GGTATCA	525	0.0	25.72381	1
ATCGCTA	40	0.0019303887	23.125	10
GTTAGCC	60	3.723435E-5	21.583332	28
GTATCAA	1370	0.0	21.335768	1
TTAGCCG	65	6.896883E-5	19.923077	29
CGAACCG	90	2.1496107E-6	18.5	31
CACGGTA	50	0.0070320275	18.5	2
AGACGAA	50	0.0070320275	18.5	30
CGTAGGC	50	0.0070320275	18.5	18
CGGTACT	50	0.0070320275	18.5	4
CGGTAAT	50	0.0070320275	18.5	1
ACGGTAC	50	0.0070320275	18.5	3
CTATACA	75	2.0657179E-4	17.266666	4
TAGCCGG	75	2.0657179E-4	17.266666	30
AACCGGG	100	5.870759E-6	16.650002	33
GCGAACC	100	5.870759E-6	16.650002	30
GGCAACT	100	5.870759E-6	16.650002	30
CATGCTA	315	0.0	16.444445	4
GCTCGGA	420	0.0	16.29762	11
>>END_MODULE