FastQCFastQC Report
Fri 10 Feb 2017
ERR1633281.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1633281.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences545558
Sequences flagged as poor quality0
Sequence length43
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT99981.8326190799145097No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT73591.3488941597410358No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT38290.7018502157424141No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT20000.3665971354099839No Hit
ATCATTAACTGAATCCATAGGTTAATGAGGCGAACCGGGGGAA15340.28118000285945766No Hit
GTATGAAACTGCTCGCCGGCAGCTTCGCCGCTCTCTTCCTGAG14670.2688989988232232No Hit
GTACTGGTTCACTATCGGTCAGTCAGGAGTATTTAGCCTTGGA13670.250569142052724No Hit
CCGTATTACCGCGGCTGCTGGCACGGAGTTAGCCGGTGCTTCT11060.20272821588172113No Hit
GTTAATGATAGTGTGTCGAAACACACTGGGTTTCCCCATTCGG10670.19557957174122642No Hit
TCTTTAGTGACCATGAAGTGCGATTGCCTCGGTGATAACGACA9880.18109898489253204No Hit
ATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTT9710.1779829092415472No Hit
GTATGAAACTGCTTGTCCGGCGAGTCGCCGTCCGCCGCCTGGG8990.16478541236678776No Hit
ATACAGGGTGACAGCCCCGTACACAAAAATGCACATGCTGTGA8790.16111944101268794No Hit
GTTAATACCTTTGCTCATTGACGTTACCCGCAGAAGAAGCACC8460.15507058827842318No Hit
ACCATGAAGTGCGATTGCCTCGGTGATAACGACATTGGGGCCA7510.13765722434644898No Hit
TATTCAGACAGGATACCACGTGTCCCGCCCTACTCATCGAGCT7420.13600753723710404No Hit
TCTTTAGTGGCCCCAATGTCGTTATCACCGAGGCAATCGCACT6630.12152695038840967No Hit
GAGTTAGCCGGTGCTTCTTCTGCGGGTAACGTCAATGAGCAAA6400.11731108333119485No Hit
GGATACCACGTGTCCCGCCCTACTCATCGAGCTCACAGCATGT6340.11621129192496489No Hit
GGTGATATGAACCGTTATAACCGGCGATTTCCGAATGGGGAAA6220.114011709112505No Hit
TCATTAACCTATGGATTCAGTTAATGATAGTGTGTCGAAACAC6200.113645111977095No Hit
GCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT6190.11346181340939003No Hit
CATCTTTAGTGACCATGAAGTGCGATTGCCTCGGTGATAACGA6140.11254532057086507No Hit
GGTATTAACTTTACTCCCTTCCTCCCCGCTGAAAGTACTTTAC6060.11107893202922513No Hit
GTATCAACGCAGAGTACGGGGATTTCACATCTGACTTAACAAA6040.11071233489381514No Hit
CCGTCATCTTTAGTGACCATGAAGTGCGATTGCCTCGGTGATA5950.10906264778447021No Hit
GGTTAATGAGGCGAACCGGGGGAACTGAAACATCTAAGTACCC5950.10906264778447021No Hit
GTATTTAGCCTTGGAGGATGGTCCCCCCATATTCAGACAGGAT5930.10869605064906024No Hit
CCCATATTCAGACAGGATACCACGTGTCCCGCCCTACTCATCG5880.10777955781053528No Hit
GTGGATGCCCTGGCAGTCAGAGGCGATGAAGGACGTGCTAATC5840.1070463635397153No Hit
GTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT5750.10539667643037039No Hit
CTATCGGTCAGTCAGGAGTATTTAGCCTTGGAGGATGGTCCCC5600.1026471979147955No Hit
CTCCAGGACAGGCTGCATCAGAAGAGGCCATCAAGCAGATCAC5490.1006309136700406No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTTATGC250.005493843529.61
TAGTACA250.005493843529.64
TGGTACT358.863397E-426.4285725
GGTATCA16350.024.6666681
GTATTGC451.3219676E-424.6666661
TTAACGG1150.024.13043635
ACTAGTG701.9190702E-723.7857158
AACGGCC1250.023.68000237
GACACAT1350.023.29629726
GTGACAC1350.023.29629724
CTAGTGC400.001929894223.1259
CATAATA802.7192073E-823.1252
GTATCAA42100.022.9821831
GTTTAAC1300.022.7692333
TAAGAAC502.699581E-422.23
TTCGCCG4250.021.76470624
AGTGACA1450.021.68965523
TCGTCTG603.7216687E-521.58333423
GCCGCTC4150.021.3975927
TGCTCGC4600.021.31521810
Produced by FastQC (version 0.11.5)