##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	ERR1633281.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	545558
Sequences flagged as poor quality	0
Sequence length	43
%GC	46
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.79215592109363	33.0	33.0	33.0	27.0	33.0
2	31.902992899013487	33.0	33.0	33.0	33.0	33.0
3	31.61842003966581	33.0	33.0	33.0	27.0	33.0
4	31.90633993085978	33.0	33.0	33.0	33.0	33.0
5	32.015838829235385	33.0	33.0	33.0	33.0	33.0
6	35.109071812712855	37.0	37.0	37.0	33.0	37.0
7	35.28934778703639	37.0	37.0	37.0	33.0	37.0
8	35.44901733637853	37.0	37.0	37.0	33.0	37.0
9	35.57829415020951	37.0	37.0	37.0	33.0	37.0
10	35.537220607158176	37.0	37.0	37.0	33.0	37.0
11	35.55021830859414	37.0	37.0	37.0	33.0	37.0
12	35.5468511139054	37.0	37.0	37.0	33.0	37.0
13	35.562438824103026	37.0	37.0	37.0	33.0	37.0
14	35.54926698902775	37.0	37.0	37.0	33.0	37.0
15	35.58142488974591	37.0	37.0	37.0	33.0	37.0
16	35.56298688682047	37.0	37.0	37.0	33.0	37.0
17	35.544446236697105	37.0	37.0	37.0	33.0	37.0
18	35.52370600376128	37.0	37.0	37.0	33.0	37.0
19	35.51089343387871	37.0	37.0	37.0	33.0	37.0
20	35.51392519218855	37.0	37.0	37.0	33.0	37.0
21	35.504105887916594	37.0	37.0	37.0	33.0	37.0
22	35.40487354231814	37.0	37.0	37.0	33.0	37.0
23	35.44501959461689	37.0	37.0	37.0	33.0	37.0
24	35.44135912221982	37.0	37.0	37.0	33.0	37.0
25	35.4570311497586	37.0	37.0	37.0	33.0	37.0
26	35.3468998713244	37.0	37.0	37.0	33.0	37.0
27	35.35485686214848	37.0	37.0	37.0	33.0	37.0
28	35.37100179999194	37.0	37.0	37.0	33.0	37.0
29	35.36943276425238	37.0	37.0	37.0	33.0	37.0
30	35.35762467052083	37.0	37.0	37.0	33.0	37.0
31	35.33225798173613	37.0	37.0	37.0	33.0	37.0
32	35.305457898151985	37.0	37.0	37.0	33.0	37.0
33	35.26591856411234	37.0	37.0	37.0	33.0	37.0
34	35.21169518181385	37.0	37.0	37.0	33.0	37.0
35	35.12433325145997	37.0	37.0	37.0	33.0	37.0
36	35.10897833044333	37.0	37.0	37.0	33.0	37.0
37	35.07916298542043	37.0	37.0	37.0	33.0	37.0
38	35.012075709640406	37.0	37.0	37.0	27.0	37.0
39	34.87923007269621	37.0	37.0	37.0	27.0	37.0
40	34.614389304161975	37.0	37.0	37.0	27.0	37.0
41	34.726272916903426	37.0	37.0	37.0	27.0	37.0
42	34.684350701483616	37.0	37.0	37.0	27.0	37.0
43	34.303610248589514	37.0	37.0	37.0	27.0	37.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
14	4.0
15	22.0
16	49.0
17	35.0
18	36.0
19	23.0
20	49.0
21	141.0
22	329.0
23	733.0
24	1465.0
25	2617.0
26	4084.0
27	6081.0
28	8592.0
29	11691.0
30	15420.0
31	19616.0
32	24124.0
33	31315.0
34	43870.0
35	85395.0
36	289867.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	44.89586808368679	18.05050975331679	14.236433156511316	22.8171890064851
2	16.5124514717042	22.5086975170376	38.487750156720274	22.491100854537923
3	19.044537885980958	28.039731797535733	29.96839932692766	22.94733098955565
4	12.058112977905191	17.387702132495537	39.31314360709585	31.241041282503417
5	14.80355892499056	35.909472503381856	32.38464104641486	16.90232752521272
6	27.93525161394389	40.395521649393835	16.090681467415013	15.578545269247265
7	26.10556531111266	33.27125621840391	21.174467242712964	19.448711227770467
8	26.313792484025527	32.148735789778534	19.62577764417349	21.911694082022443
9	28.758812078642414	13.096132766818561	18.75712573181953	39.387929422719495
10	16.869883678728932	26.671774586753376	30.395301691112586	26.0630400434051
11	35.03037257266872	23.74907892469728	21.621715747913147	19.598832754720856
12	25.330212369720545	26.080270108769373	27.43411332983844	21.155404191671646
13	30.668416556993023	22.22440143852716	24.939969719076615	22.167212285403203
14	24.016328236411162	20.757279702616405	28.916632145436417	26.30975991553602
15	26.21206177894926	25.991553602000156	26.576459331546783	21.219925287503806
16	21.53813160103967	27.619244883220485	25.40518148391189	25.437442031827963
17	22.944031615336957	24.84795383808871	25.436342240421734	26.7716723061526
18	25.29410255188266	22.51456307120416	28.989035079679887	23.202299297233292
19	27.335132103277743	23.011485488252394	29.145938653635362	20.5074437548345
20	26.524035941183154	22.267476601937833	29.073719017959593	22.13476843891942
21	23.98553407703672	24.792414372074095	29.12559251262011	22.096459038269074
22	23.610321908944602	24.5170999233812	29.015979969132523	22.85659819854168
23	23.15079239970819	24.3922735987741	30.012207684609155	22.444726316908557
24	23.436921463895683	24.445613481976253	29.33546937264232	22.781995681485746
25	23.086637901011443	24.696915818299793	30.602062475483816	21.614383805204945
26	23.669893943448724	24.70589744811734	29.41098838253678	22.213220225897153
27	23.997448483937546	24.224372110756327	29.561109909487165	22.217069495818958
28	22.350510853108194	24.636243992389442	29.39155873436005	23.621686420142314
29	23.8267975174042	24.43461556791395	28.83598077564622	22.90260613903563
30	23.78537204110287	24.1792806631009	29.969315819766184	22.066031476030044
31	23.034947704918636	24.406570887055086	29.424369177979244	23.134112230047034
32	21.68733663515153	24.360929543696546	29.91304315948075	24.03869066167117
33	22.100858203893996	24.601417264525498	30.220434857522022	23.077289674058488
34	22.260144659229635	24.87251584616118	29.68556963695886	23.181769857650334
35	22.4205309059715	25.441841197452884	29.30614160180952	22.831486294766094
36	23.039346870543554	24.967464504232364	29.427485253630227	22.565703371593855
37	23.236392830826418	25.300884598887745	29.00278247225776	22.459940098028074
38	23.275985321450698	25.187422785478354	28.121299660164457	23.41529223290649
39	22.54022487068286	24.693249846945694	28.838180358458683	23.928344923912764
40	22.66945036091488	25.868560263070105	29.15070441639569	22.311284959619325
41	23.08480491533439	24.61369826856173	28.790156133719968	23.511340682383906
42	21.84094083488832	26.072021673222643	28.883638403249517	23.20339908863952
43	22.64268877002995	25.279255367898557	28.55003501002643	23.52802085204506
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	16.0
1	61.5
2	107.0
3	496.5
4	886.0
5	886.0
6	1181.0
7	1476.0
8	1447.5
9	1419.0
10	1930.0
11	2441.0
12	2441.0
13	3929.0
14	5417.0
15	9069.0
16	12721.0
17	13513.5
18	14306.0
19	14306.0
20	11265.0
21	8224.0
22	6274.0
23	4324.0
24	4531.5
25	4739.0
26	4739.0
27	5340.5
28	5942.0
29	6637.5
30	7333.0
31	8692.0
32	10051.0
33	10051.0
34	12859.0
35	15667.0
36	17054.5
37	18442.0
38	21115.0
39	23788.0
40	23788.0
41	26284.0
42	28780.0
43	31623.5
44	34467.0
45	37910.0
46	41353.0
47	41353.0
48	43446.0
49	45539.0
50	48514.5
51	51490.0
52	51062.0
53	50634.0
54	50634.0
55	46459.5
56	42285.0
57	37444.0
58	32603.0
59	29555.5
60	26508.0
61	26508.0
62	22742.0
63	18976.0
64	14787.0
65	10598.0
66	9420.0
67	8242.0
68	8242.0
69	6932.5
70	5623.0
71	4757.5
72	3892.0
73	3765.5
74	3639.0
75	3639.0
76	2813.0
77	1987.0
78	1417.5
79	848.0
80	554.5
81	261.0
82	261.0
83	218.0
84	175.0
85	178.5
86	182.0
87	140.0
88	98.0
89	98.0
90	79.5
91	61.0
92	37.5
93	14.0
94	9.0
95	4.0
96	4.0
97	2.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
43	545558.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	39.15528213603952
#Duplication Level	Percentage of deduplicated	Percentage of total
1	82.31081862037976	32.229033259293445
2	8.75312995732674	6.854625461050961
3	2.686331235458633	3.155521723057151
4	1.376657760584957	2.1561369208188936
5	0.8197030523967875	1.6047852142184498
6	0.5741793847293546	1.3489293483465277
7	0.39130024248514794	1.072502997608465
8	0.32673776246876846	1.0234807419170313
9	0.25685457303961107	0.9051491947768147
>10	1.9714635576145123	15.789867643874159
>50	0.30344676859417163	8.415688144607294
>100	0.20978445891783687	16.037090405898272
>500	0.015291805661436857	3.844942430209238
>1k	0.0033450824884393125	2.3145621617445267
>5k	9.557378538398036E-4	3.2476843525787995
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	fail
#Sequence	Count	Percentage	Possible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT	9998	1.8326190799145097	No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT	7359	1.3488941597410358	No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT	3829	0.7018502157424141	No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	2000	0.3665971354099839	No Hit
ATCATTAACTGAATCCATAGGTTAATGAGGCGAACCGGGGGAA	1534	0.28118000285945766	No Hit
GTATGAAACTGCTCGCCGGCAGCTTCGCCGCTCTCTTCCTGAG	1467	0.2688989988232232	No Hit
GTACTGGTTCACTATCGGTCAGTCAGGAGTATTTAGCCTTGGA	1367	0.250569142052724	No Hit
CCGTATTACCGCGGCTGCTGGCACGGAGTTAGCCGGTGCTTCT	1106	0.20272821588172113	No Hit
GTTAATGATAGTGTGTCGAAACACACTGGGTTTCCCCATTCGG	1067	0.19557957174122642	No Hit
TCTTTAGTGACCATGAAGTGCGATTGCCTCGGTGATAACGACA	988	0.18109898489253204	No Hit
ATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTT	971	0.1779829092415472	No Hit
GTATGAAACTGCTTGTCCGGCGAGTCGCCGTCCGCCGCCTGGG	899	0.16478541236678776	No Hit
ATACAGGGTGACAGCCCCGTACACAAAAATGCACATGCTGTGA	879	0.16111944101268794	No Hit
GTTAATACCTTTGCTCATTGACGTTACCCGCAGAAGAAGCACC	846	0.15507058827842318	No Hit
ACCATGAAGTGCGATTGCCTCGGTGATAACGACATTGGGGCCA	751	0.13765722434644898	No Hit
TATTCAGACAGGATACCACGTGTCCCGCCCTACTCATCGAGCT	742	0.13600753723710404	No Hit
TCTTTAGTGGCCCCAATGTCGTTATCACCGAGGCAATCGCACT	663	0.12152695038840967	No Hit
GAGTTAGCCGGTGCTTCTTCTGCGGGTAACGTCAATGAGCAAA	640	0.11731108333119485	No Hit
GGATACCACGTGTCCCGCCCTACTCATCGAGCTCACAGCATGT	634	0.11621129192496489	No Hit
GGTGATATGAACCGTTATAACCGGCGATTTCCGAATGGGGAAA	622	0.114011709112505	No Hit
TCATTAACCTATGGATTCAGTTAATGATAGTGTGTCGAAACAC	620	0.113645111977095	No Hit
GCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	619	0.11346181340939003	No Hit
CATCTTTAGTGACCATGAAGTGCGATTGCCTCGGTGATAACGA	614	0.11254532057086507	No Hit
GGTATTAACTTTACTCCCTTCCTCCCCGCTGAAAGTACTTTAC	606	0.11107893202922513	No Hit
GTATCAACGCAGAGTACGGGGATTTCACATCTGACTTAACAAA	604	0.11071233489381514	No Hit
CCGTCATCTTTAGTGACCATGAAGTGCGATTGCCTCGGTGATA	595	0.10906264778447021	No Hit
GGTTAATGAGGCGAACCGGGGGAACTGAAACATCTAAGTACCC	595	0.10906264778447021	No Hit
GTATTTAGCCTTGGAGGATGGTCCCCCCATATTCAGACAGGAT	593	0.10869605064906024	No Hit
CCCATATTCAGACAGGATACCACGTGTCCCGCCCTACTCATCG	588	0.10777955781053528	No Hit
GTGGATGCCCTGGCAGTCAGAGGCGATGAAGGACGTGCTAATC	584	0.1070463635397153	No Hit
GTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	575	0.10539667643037039	No Hit
CTATCGGTCAGTCAGGAGTATTTAGCCTTGGAGGATGGTCCCC	560	0.1026471979147955	No Hit
CTCCAGGACAGGCTGCATCAGAAGAGGCCATCAAGCAGATCAC	549	0.1006309136700406	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0012830899739349436	0.0	0.0
2	0.0	0.0	0.0018329856770499195	0.0	0.0
3	0.0	0.0	0.0029327770832798714	0.0	0.0
4	0.0	0.0	0.003665971354099839	1.8329856770499196E-4	0.0
5	0.0	0.0	0.004765762760329791	1.8329856770499196E-4	0.0
6	0.0	0.0	0.00934822695295459	1.8329856770499196E-4	0.0
7	0.0	0.0	0.01979624531213913	3.665971354099839E-4	0.0
8	0.0	0.0	0.02437870950476393	3.665971354099839E-4	0.0
9	1.8329856770499196E-4	0.0	0.030794159374438648	0.0012830899739349436	0.0
10	0.0016496871093449276	0.0	0.03574322070247343	0.0012830899739349436	0.0
11	0.0018329856770499195	0.0	0.05132359895739775	0.0012830899739349436	0.0
12	0.0018329856770499195	0.0	0.05663925742084251	0.0014663885416399357	0.0
13	0.0018329856770499195	0.0	0.05938873593641739	0.0016496871093449276	0.0
14	0.0018329856770499195	0.0	0.06360460299363221	0.0018329856770499195	0.0
15	0.0018329856770499195	0.0	0.07020335143101192	0.0020162842447549113	0.0
16	0.0020162842447549113	0.0	0.07570230846216168	0.0020162842447549113	0.0
17	0.0020162842447549113	0.0	0.0773519955715066	0.0021995828124599034	0.0
18	0.0021995828124599034	0.0	0.07955157838396651	0.0023828813801648955	0.0
19	0.0021995828124599034	0.0	0.08065136979019646	0.0023828813801648955	0.0
20	0.002566179947869887	0.0	0.08230105689954138	0.0034826727863948473	0.0
21	0.0027494785155748793	0.0	0.08578372968593623	0.005315658463444767	0.0
22	0.0027494785155748793	0.0	0.08780001393069115	0.006782047005084702	0.0
23	0.0027494785155748793	0.0	0.08834990963380612	0.009531525520659582	0.0
24	0.0027494785155748793	0.0	0.08853320820151112	0.012281004036234461	0.0
25	0.0027494785155748793	0.0	0.08871650676921611	0.013747392577874397	0.0
26	0.0027494785155748793	0.0	0.08871650676921611	0.015580378254924317	0.0
27	0.0027494785155748793	0.0	0.08871650676921611	0.017230065364269243	0.0
28	0.0029327770832798714	0.0	0.08871650676921611	0.041792073436738165	0.0
29	0.0029327770832798714	0.0	0.08871650676921611	0.09274907525872593	0.0
30	0.0029327770832798714	0.0	0.08871650676921611	0.16753489088236265	0.0
31	0.0029327770832798714	0.0	0.08871650676921611	0.28612906418749245	0.0
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GTTATGC	25	0.0054938435	29.6	1
TAGTACA	25	0.0054938435	29.6	4
TGGTACT	35	8.863397E-4	26.428572	5
GGTATCA	1635	0.0	24.666668	1
GTATTGC	45	1.3219676E-4	24.666666	1
TTAACGG	115	0.0	24.130436	35
ACTAGTG	70	1.9190702E-7	23.785715	8
AACGGCC	125	0.0	23.680002	37
GACACAT	135	0.0	23.296297	26
GTGACAC	135	0.0	23.296297	24
CTAGTGC	40	0.0019298942	23.125	9
CATAATA	80	2.7192073E-8	23.125	2
GTATCAA	4210	0.0	22.982183	1
GTTTAAC	130	0.0	22.76923	33
TAAGAAC	50	2.699581E-4	22.2	3
TTCGCCG	425	0.0	21.764706	24
AGTGACA	145	0.0	21.689655	23
TCGTCTG	60	3.7216687E-5	21.583334	23
GCCGCTC	415	0.0	21.39759	27
TGCTCGC	460	0.0	21.315218	10
>>END_MODULE