FastQCFastQC Report
Fri 10 Feb 2017
ERR1633280.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1633280.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences947859
Sequences flagged as poor quality0
Sequence length43
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT47380.4998633763038596No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT34460.36355618293438163No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT19960.21057984362653093No Hit
ATTGAAAGCTGAGTATTTTTAAGACAAAGGTTTCAGGAAGAAA17590.1855761247189719No Hit
GTGATATAGTTATTTCCTGTCAGTGATTTTGGTCTGAATCAAC15570.1642649381395334No Hit
GAATACACCTCTTAAATTTACAGGACTTAACATTTCAAACATC15430.1627879252082852No Hit
GTTCTACAGCACACTACCAGAAGACAGCAGAAATGAAAAGCAT15130.1596228974984676No Hit
ATCTTGAATAGTGATATAGTTATTTCCTGTCAGTGATTTTGGT12190.1286056259422551No Hit
ACTTTATTCCATTTGTAATTTTTGGCTACACAACACTAAAAGA12180.12850012501859454No Hit
ATTCAAGATCATCTTCACAACATCACCTGCTAGCCACGTGGGA12050.12712861301100692No Hit
TCCCAGAAGAGGTCGCCATTGTTGAAGAACTTGGCCGCAGACA11420.12048205482038996No Hit
GCTTAGGACACAGAGCACATCAAAAGTTCCCAAAGAGGGCTTG11140.11752802895789352No Hit
GTTTATAAAGTCCCTGGCGGCAAGATTATCAAGAATGGTGTTC10790.113835496629773No Hit
CTATATCACTATTCAAGATCATCTTCACAACATCACCTGCTAG10590.11172547815656127No Hit
GTTATTTCCTGTCAGTGATTTTGGTCTGAATCAACCAGTTTAT10340.1090879550650466No Hit
CTCTTCACCTGCTCTGTTCTACAGCACACTACCAGAAGACAGC10030.10581742643156841No Hit
CTACCAGAAGACAGCAGAAATGAAAAGCATTTACTTTGTGGCT9580.101069884866842No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA10100.025.277231
TAGCGCT400.001930938923.1259
TCCGCAC603.7254E-521.58333225
GTATCAA24850.021.4406451
AGTGCGC555.1421806E-420.1818183
CTCGTCT1254.129106E-1019.24000213
ACCGACC1355.638867E-1119.1851868
TCGCCAT2000.018.513
CGAGTCG902.151406E-618.521
GGACGTA500.007033994418.527
CTCGAAC500.007033994418.53
CTTAGCG701.218911E-418.524
GTCGCCC1700.018.49999837
GTCGCCA2050.018.0487812
CGATAAG953.605037E-617.52631612
CGCCGTC852.7223008E-517.41176426
GCCGTCC852.7223008E-517.41176427
GTTCGGC752.0667847E-417.26666635
GTCCGCA752.0667847E-417.26666624
AGTTCGG752.0667847E-417.26666634
Produced by FastQC (version 0.11.5)