##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	ERR1633280.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	947859
Sequences flagged as poor quality	0
Sequence length	43
%GC	44
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.69175056627621	33.0	33.0	33.0	27.0	33.0
2	31.850926139858355	33.0	33.0	33.0	33.0	33.0
3	31.57390286951962	33.0	33.0	33.0	27.0	33.0
4	31.857918741078578	33.0	33.0	33.0	27.0	33.0
5	31.97533599406663	33.0	33.0	33.0	33.0	33.0
6	35.106831290307944	37.0	37.0	37.0	33.0	37.0
7	35.27031341159392	37.0	37.0	37.0	33.0	37.0
8	35.41388539856666	37.0	37.0	37.0	33.0	37.0
9	35.53069602124366	37.0	37.0	37.0	33.0	37.0
10	35.54633969820406	37.0	37.0	37.0	33.0	37.0
11	35.56345194802181	37.0	37.0	37.0	33.0	37.0
12	35.55668406376898	37.0	37.0	37.0	33.0	37.0
13	35.55513531020964	37.0	37.0	37.0	33.0	37.0
14	35.532713198904055	37.0	37.0	37.0	33.0	37.0
15	35.54289931308349	37.0	37.0	37.0	33.0	37.0
16	35.52156491630084	37.0	37.0	37.0	33.0	37.0
17	35.52625232233908	37.0	37.0	37.0	33.0	37.0
18	35.51395407966797	37.0	37.0	37.0	33.0	37.0
19	35.504380925855	37.0	37.0	37.0	33.0	37.0
20	35.50879191947325	37.0	37.0	37.0	33.0	37.0
21	35.50242177370263	37.0	37.0	37.0	33.0	37.0
22	35.42150995031962	37.0	37.0	37.0	33.0	37.0
23	35.45625984455494	37.0	37.0	37.0	33.0	37.0
24	35.44150554038101	37.0	37.0	37.0	33.0	37.0
25	35.46079005421692	37.0	37.0	37.0	33.0	37.0
26	35.34756857296286	37.0	37.0	37.0	33.0	37.0
27	35.373527075229546	37.0	37.0	37.0	33.0	37.0
28	35.38615870081943	37.0	37.0	37.0	33.0	37.0
29	35.40151541526746	37.0	37.0	37.0	33.0	37.0
30	35.3960578524865	37.0	37.0	37.0	33.0	37.0
31	35.38411831295583	37.0	37.0	37.0	33.0	37.0
32	35.37415586073456	37.0	37.0	37.0	33.0	37.0
33	35.371692414167086	37.0	37.0	37.0	33.0	37.0
34	35.34127438785727	37.0	37.0	37.0	33.0	37.0
35	35.28895647981398	37.0	37.0	37.0	33.0	37.0
36	35.321375858645645	37.0	37.0	37.0	33.0	37.0
37	35.31830472675788	37.0	37.0	37.0	33.0	37.0
38	35.306334591959356	37.0	37.0	37.0	33.0	37.0
39	35.19742915349224	37.0	37.0	37.0	33.0	37.0
40	34.98542082735934	37.0	37.0	37.0	27.0	37.0
41	35.149412518106594	37.0	37.0	37.0	33.0	37.0
42	35.1957622388984	37.0	37.0	37.0	33.0	37.0
43	34.84598658661257	37.0	37.0	37.0	27.0	37.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
14	11.0
15	26.0
16	56.0
17	59.0
18	30.0
19	46.0
20	79.0
21	195.0
22	543.0
23	1257.0
24	2469.0
25	4323.0
26	6860.0
27	10303.0
28	14322.0
29	19204.0
30	24801.0
31	31528.0
32	39665.0
33	51919.0
34	74273.0
35	149591.0
36	516299.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	40.73010859210072	21.389468264794658	14.235239629522955	23.64518351358166
2	15.227264814703453	22.687340627667197	40.03032096546006	22.0550735921693
3	17.534675516084146	27.997624119199166	31.65069910187064	22.817001262846055
4	12.366501768722985	18.450740036229014	39.976304492545836	29.206453702502166
5	12.953825410741471	38.224672657009116	35.75035949439737	13.071142437852043
6	27.876509058836813	40.79857869155644	18.018608252915254	13.30630399669149
7	24.586568255405076	33.591916097225436	24.314481373284423	17.507034274085072
8	23.500330745395676	36.791126106309065	21.312030586827788	18.396512561467475
9	25.291103423610473	16.05048852202701	20.849831040270757	37.80857701409176
10	14.128683696625762	29.052000350263068	34.841469037061415	21.97784691604975
11	32.25553589721678	24.744503138124973	25.049506308427734	17.950454656230516
12	21.670733727273785	26.945463407532134	31.070866025432053	20.312936839762035
13	26.964770076562022	23.22581734203083	27.548612188099707	22.26080039330744
14	21.369634091146466	22.242548733514163	28.784872011554462	27.60294516378491
15	23.214423242275487	29.46324295069203	25.86629445940799	21.45603934762449
16	21.889120639251196	29.832496183504087	25.774403154899623	22.503980022345097
17	21.66809620418227	28.47174527012984	27.023006586422664	22.83715193926523
18	21.726965719584875	27.91934243384301	28.902611042359677	21.45108080421244
19	23.786343749439524	27.213330252706363	29.148217192641525	19.85210880521259
20	23.64666052651291	26.605433930574062	29.221118330890988	20.52678721202204
21	21.696581453570627	28.39472959585761	28.07421778977675	21.83447116079501
22	21.336401300193383	28.31549840218851	28.254518868312694	22.093581429305413
23	21.437787687831207	27.975890928925086	28.84542954173564	21.740891841508073
24	22.67774004361408	28.044888532999106	27.881362101325198	21.396009322061616
25	22.37853942411266	27.470647005514532	28.592754829568534	21.558058740804277
26	22.10339301520585	27.60505518225812	28.359597788278634	21.931954014257396
27	21.98850250933947	27.531310036619374	28.33016303057733	22.150024423463826
28	21.10609278384232	28.063667697410693	28.9108401144052	21.919399404341785
29	22.51326410362723	28.033072429549122	27.889802175218044	21.56386129160561
30	21.70206750160098	28.263908450518482	28.387344531201368	21.646679516679168
31	22.251832814796295	27.688928416568288	28.60130040438504	21.457938364250378
32	21.13637154893291	27.710134102224064	28.733598562655416	22.419895786187606
33	21.388413255558053	27.564331825725137	29.217953303181172	21.82930161553564
34	21.440741713693704	27.687134900866056	28.551398467493584	22.32072491794666
35	21.812948972368254	28.27762357059436	28.144692406782024	21.764735050255364
36	22.172496120203533	28.107450580729836	27.988551039764353	21.731502259302278
37	22.532676273580776	27.15129570959394	27.945084659216192	22.370943357609097
38	22.41472624092824	27.88199510686716	28.101859031775824	21.60141962042878
39	21.974681888339934	27.576675433793422	28.471112264587877	21.977530413278767
40	22.026588342780943	27.598197622220184	28.560893550623035	21.81432048437584
41	21.629588367046154	27.64219150738665	28.692453202427785	22.035766923139413
42	21.859685881549893	27.569923374679146	28.423953351711596	22.14643739205937
43	21.735089290706743	27.423488092638248	28.319718439134935	22.521704177520075
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	34.0
1	57.5
2	81.0
3	281.0
4	481.0
5	481.0
6	594.0
7	707.0
8	690.5
9	674.0
10	1017.5
11	1361.0
12	1361.0
13	2066.5
14	2772.0
15	5286.5
16	7801.0
17	8868.5
18	9936.0
19	9936.0
20	9934.5
21	9933.0
22	11630.0
23	13327.0
24	16418.5
25	19510.0
26	19510.0
27	23328.5
28	27147.0
29	34344.0
30	41541.0
31	43763.0
32	45985.0
33	45985.0
34	50258.0
35	54531.0
36	57053.5
37	59576.0
38	64160.0
39	68744.0
40	68744.0
41	72535.0
42	76326.0
43	71643.0
44	66960.0
45	67386.5
46	67813.0
47	67813.0
48	66383.0
49	64953.0
50	66798.0
51	68643.0
52	67586.5
53	66530.0
54	66530.0
55	57498.0
56	48466.0
57	42546.0
58	36626.0
59	32160.5
60	27695.0
61	27695.0
62	24200.0
63	20705.0
64	17082.5
65	13460.0
66	11431.0
67	9402.0
68	9402.0
69	7766.5
70	6131.0
71	5157.5
72	4184.0
73	3367.5
74	2551.0
75	2551.0
76	1949.5
77	1348.0
78	1063.0
79	778.0
80	606.5
81	435.0
82	435.0
83	351.5
84	268.0
85	243.5
86	219.0
87	174.0
88	129.0
89	129.0
90	99.5
91	70.0
92	46.0
93	22.0
94	13.0
95	4.0
96	4.0
97	2.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
43	947859.0
>>END_MODULE
>>Sequence Duplication Levels	warn
#Total Deduplicated Percentage	55.51029119066222
#Duplication Level	Percentage of deduplicated	Percentage of total
1	87.9721480535736	48.83359555121919
2	7.238318501019074	8.036023354446527
3	1.6875946029885112	2.8103660347104675
4	0.7171889909294473	1.592454789009233
5	0.42457700769785894	1.1784196665084092
6	0.2974500781390076	0.9906924271308922
7	0.20240568170035406	0.7864918830881801
8	0.15014455618148026	0.6667654427461369
9	0.13174830501839743	0.6582048097902683
>10	0.9063842952950648	10.309366204488834
>50	0.13930618142919654	5.442944347990808
>100	0.12288014917104888	13.4374850814072
>500	0.006762272352829415	2.4016537760483696
>1k	0.0030913245041505892	2.855536631415525
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT	4738	0.4998633763038596	No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT	3446	0.36355618293438163	No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT	1996	0.21057984362653093	No Hit
ATTGAAAGCTGAGTATTTTTAAGACAAAGGTTTCAGGAAGAAA	1759	0.1855761247189719	No Hit
GTGATATAGTTATTTCCTGTCAGTGATTTTGGTCTGAATCAAC	1557	0.1642649381395334	No Hit
GAATACACCTCTTAAATTTACAGGACTTAACATTTCAAACATC	1543	0.1627879252082852	No Hit
GTTCTACAGCACACTACCAGAAGACAGCAGAAATGAAAAGCAT	1513	0.1596228974984676	No Hit
ATCTTGAATAGTGATATAGTTATTTCCTGTCAGTGATTTTGGT	1219	0.1286056259422551	No Hit
ACTTTATTCCATTTGTAATTTTTGGCTACACAACACTAAAAGA	1218	0.12850012501859454	No Hit
ATTCAAGATCATCTTCACAACATCACCTGCTAGCCACGTGGGA	1205	0.12712861301100692	No Hit
TCCCAGAAGAGGTCGCCATTGTTGAAGAACTTGGCCGCAGACA	1142	0.12048205482038996	No Hit
GCTTAGGACACAGAGCACATCAAAAGTTCCCAAAGAGGGCTTG	1114	0.11752802895789352	No Hit
GTTTATAAAGTCCCTGGCGGCAAGATTATCAAGAATGGTGTTC	1079	0.113835496629773	No Hit
CTATATCACTATTCAAGATCATCTTCACAACATCACCTGCTAG	1059	0.11172547815656127	No Hit
GTTATTTCCTGTCAGTGATTTTGGTCTGAATCAACCAGTTTAT	1034	0.1090879550650466	No Hit
CTCTTCACCTGCTCTGTTCTACAGCACACTACCAGAAGACAGC	1003	0.10581742643156841	No Hit
CTACCAGAAGACAGCAGAAATGAAAAGCATTTACTTTGTGGCT	958	0.101069884866842	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	1.0550092366058664E-4	1.0550092366058664E-4	0.0
2	0.0	0.0	1.0550092366058664E-4	2.110018473211733E-4	0.0
3	0.0	0.0	2.110018473211733E-4	2.110018473211733E-4	0.0
4	0.0	0.0	3.1650277098175996E-4	3.1650277098175996E-4	0.0
5	0.0	0.0	6.330055419635199E-4	3.1650277098175996E-4	0.0
6	0.0	0.0	0.001793515702229973	3.1650277098175996E-4	0.0
7	0.0	0.0	0.0037980332517811195	4.220036946423466E-4	0.0
8	0.0	0.0	0.0044310387937446395	4.220036946423466E-4	0.0
9	0.0	0.0	0.006224554495974612	0.0013715120075876264	0.0
10	4.220036946423466E-4	0.0	0.006752059114277545	0.001477012931248213	0.0
11	4.220036946423466E-4	0.0	0.009495083129452799	0.0015825138549087998	0.0
12	4.220036946423466E-4	0.0	0.010233589595076906	0.0016880147785693863	0.0
13	4.220036946423466E-4	0.0	0.011288598831682772	0.0016880147785693863	0.0
14	4.220036946423466E-4	0.0	0.011816103449985705	0.0016880147785693863	0.0
15	4.220036946423466E-4	0.0	0.013398617304894504	0.001793515702229973	0.0
16	5.275046183029332E-4	0.0	0.014664628388821543	0.0018990166258905598	0.0
17	5.275046183029332E-4	0.0	0.014875630236142717	0.0020045175495511463	0.0
18	5.275046183029332E-4	0.0	0.015297633930785063	0.0021100184732117328	0.0
19	5.275046183029332E-4	0.0	0.01571963762542741	0.0023210203205329062	0.0
20	5.275046183029332E-4	0.0	0.01635264316739093	0.0027430240151752527	0.0
21	5.275046183029332E-4	0.0	0.017513153327657384	0.0031650277098175996	0.0
22	5.275046183029332E-4	0.0	0.017829656098639145	0.0034815304807993596	0.0
23	5.275046183029332E-4	0.0	0.018040657945960315	0.0040090350991022925	0.0
24	5.275046183029332E-4	0.0	0.018146158869620902	0.0051695452593687454	0.0
25	5.275046183029332E-4	0.0	0.018146158869620902	0.005380547106689919	0.0
26	5.275046183029332E-4	0.0	0.01825165979328149	0.006435556343295785	0.0
27	5.275046183029332E-4	0.0	0.01825165979328149	0.008018070198204585	0.0
28	5.275046183029332E-4	0.0	0.018357160716942076	0.016141641320069757	0.0
29	5.275046183029332E-4	0.0	0.018357160716942076	0.03449880203701183	0.0
30	5.275046183029332E-4	0.0	0.018357160716942076	0.05517698307448682	0.0
31	5.275046183029332E-4	0.0	0.018462661640602663	0.11995455020208702	0.0
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GGTATCA	1010	0.0	25.27723	1
TAGCGCT	40	0.0019309389	23.125	9
TCCGCAC	60	3.7254E-5	21.583332	25
GTATCAA	2485	0.0	21.440645	1
AGTGCGC	55	5.1421806E-4	20.181818	3
CTCGTCT	125	4.129106E-10	19.240002	13
ACCGACC	135	5.638867E-11	19.185186	8
TCGCCAT	200	0.0	18.5	13
CGAGTCG	90	2.151406E-6	18.5	21
GGACGTA	50	0.0070339944	18.5	27
CTCGAAC	50	0.0070339944	18.5	3
CTTAGCG	70	1.218911E-4	18.5	24
GTCGCCC	170	0.0	18.499998	37
GTCGCCA	205	0.0	18.04878	12
CGATAAG	95	3.605037E-6	17.526316	12
CGCCGTC	85	2.7223008E-5	17.411764	26
GCCGTCC	85	2.7223008E-5	17.411764	27
GTTCGGC	75	2.0667847E-4	17.266666	35
GTCCGCA	75	2.0667847E-4	17.266666	24
AGTTCGG	75	2.0667847E-4	17.266666	34
>>END_MODULE