FastQCFastQC Report
Fri 10 Feb 2017
ERR1633279.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1633279.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences842106
Sequences flagged as poor quality0
Sequence length43
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT37860.4495871066112817No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT26950.32003096997290126No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT13810.1639936065055943No Hit
CCCCTATACCTTCTGCATAATGAATTAACTAGAAATAACTTTG12360.14677487157198738No Hit
GTATTAGAGGCACCGCCTGCCCAGTGACACATGTTTAACGGCC11950.14190612583213988No Hit
GTACATGCTAAGACTTCACCAGTCAAAGCGAACTACTATACTC11050.1312186351836942No Hit
TTATTACCCTCACTGTCAACCCAACACAGGCATGCTCATAAGG10940.12991238632666197No Hit
GTATGAATGGCTCCACGAGGGTTCAGCTGTCTCTTACTTTTAA9690.11506864931493184No Hit
ATATAGTACCGCAAGGGAAAGATGAAAAATTATAGCCAAGCAT9520.11304990108133654No Hit
CTCTAATACTGGTGATGCTAGAGGTGATGTTTTTGGTAAACAG9510.11293115118524272No Hit
AGCATGTACTGCTCGGAGGTTGGGTTCTGCTCCGAGGTCGCCC9460.1123374017047735No Hit
GCATAATATAGCAAGGACTAACCCCTATACCTTCTGCATAATG8650.1027186601211724No Hit
CCCTATAGAAGAACTAATGTTAGTATAAGTAACATGAAAACAT8640.10259991022507856No Hit
ATCTATCACCCTATAGAAGAACTAATGTTAGTATAAGTAACAT8470.10058116199148324No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TTACCGG358.8674173E-426.42857231
GGTATCA6100.026.3852461
GACCGTT451.3228017E-424.6666687
GTTACGG451.3228017E-424.66666817
TTTACCG400.001930760623.12500230
GTATCAA17050.023.1114371
TGCGGTA855.176662E-821.76470636
CGAGCTA909.47075E-820.55555723
CGCCGTT555.141524E-420.18181825
TACGGCC656.899329E-519.92307719
CGGGTCA902.1508258E-618.517
GCGGGTA902.1508258E-618.522
TTCGAGG500.00703335818.513
GCACCGC3050.017.59016410
TCTCGTC1900.017.52631612
TTGTCCG852.7217155E-517.41176413
CCGCCGT852.7217155E-517.41176424
ATACTTA752.0664393E-417.2666666
TTGCCGA650.001579459617.07692310
TTAACGG3150.017.03174635
Produced by FastQC (version 0.11.5)