FastQCFastQC Report
Fri 10 Feb 2017
ERR1633278.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1633278.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences888586
Sequences flagged as poor quality0
Sequence length43
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT64660.7276729545592661No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT45180.5084482537424628No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT25720.2894486296205432No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT11540.12986925294794202No Hit
GTATTAGAGGCACCGCCTGCCCAGTGACACATGTTTAACGGCC9880.11118788727258813No Hit
GATATGGACAATACTAGTTAAGAAAGCTAACAGGATGTGAAAG9160.10308512625677199No Hit
CCCCTATACCTTCTGCATAATGAATTAACTAGAAATAACTTTG9070.10207228112979498No Hit
TTATTACCCTCACTGTCAACCCAACACAGGCATGCTCATAAGG8940.1006092826130504No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA10150.023.8768481
CCGTCCG1200.023.12500228
TAAACGA400.001930844223.12529
GTATCAA27550.022.6297651
TGCGGTA705.099775E-621.14285736
GCCGCTC1151.3460522E-1020.91304427
TTAGCGC450.003825134620.55555716
AACGATG450.003825134620.55555731
CGTCCGC1450.020.41379429
CGCCGTC1403.6379788E-1219.82142826
CGAGTCG1700.019.58823621
GGCGAGT1750.019.02857219
TGTCCGG2050.018.95121814
CCGCTCT1306.9485395E-1018.528
CCGAGTT1103.8486178E-818.513
CAGCGTA500.007033657318.4999989
AGCCGCG500.007033657318.49999819
GCGGTAA500.007033657318.49999823
GTCCGAC500.007033657318.49999811
GCCGTCC1552.0008883E-1117.90322527
Produced by FastQC (version 0.11.5)