FastQCFastQC Report
Fri 10 Feb 2017
ERR1633277.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1633277.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences740547
Sequences flagged as poor quality0
Sequence length43
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT39570.534334755255237No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT28440.3840404457785934No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT15750.2126806266178919No Hit
GTATTAGAGGCACCGCCTGCCCAGTGACACATGTTTAACGGCC7620.10289691268751341No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT7610.10276187736902587No Hit
GTACATGCTAAGACTTCACCAGTCAAAGCGAACTACTATACTC7430.10033124163624996No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATCAA17150.023.839651
CTAGACA753.737041E-722.24
CGGGACT957.1340764E-921.42105317
TAGGACC1900.021.4210534
GGTATCA6250.021.0161
TTTACCG450.003824542720.55555530
TATTACG450.003824542720.5555552
GCCGGCA1450.020.41379415
TTCGCCG1550.020.29032324
AGCTTCG1850.019.00000221
GCTTCGC1850.019.00000222
AGGACCG801.6154047E-518.55
GCCGCTC1700.018.527
CCGAGTT701.2184538E-418.513
CGACACG500.00703257818.49999821
ACTAGAC500.00703257818.4999983
GTACTAG500.00703257818.4999981
TGTACGA500.00703257818.4999982
TTAACGG2050.018.04878235
TAACGGC2100.017.61904736
Produced by FastQC (version 0.11.5)