##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	ERR1633277.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	740547
Sequences flagged as poor quality	0
Sequence length	43
%GC	45
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.747633843631803	33.0	33.0	33.0	27.0	33.0
2	31.880476188547114	33.0	33.0	33.0	33.0	33.0
3	31.614391794173766	33.0	33.0	33.0	27.0	33.0
4	31.906026221158143	33.0	33.0	33.0	33.0	33.0
5	31.99931807164164	33.0	33.0	33.0	33.0	33.0
6	35.14465793528297	37.0	37.0	37.0	33.0	37.0
7	35.28467875772908	37.0	37.0	37.0	33.0	37.0
8	35.42401360075728	37.0	37.0	37.0	33.0	37.0
9	35.54498229011798	37.0	37.0	37.0	33.0	37.0
10	35.547678945428174	37.0	37.0	37.0	33.0	37.0
11	35.56178068373783	37.0	37.0	37.0	33.0	37.0
12	35.55585398360941	37.0	37.0	37.0	33.0	37.0
13	35.55654671479326	37.0	37.0	37.0	33.0	37.0
14	35.54225052562498	37.0	37.0	37.0	33.0	37.0
15	35.543282195458225	37.0	37.0	37.0	33.0	37.0
16	35.52132815337852	37.0	37.0	37.0	33.0	37.0
17	35.51927966759706	37.0	37.0	37.0	33.0	37.0
18	35.51834657354631	37.0	37.0	37.0	33.0	37.0
19	35.50049220373589	37.0	37.0	37.0	33.0	37.0
20	35.50508745562402	37.0	37.0	37.0	33.0	37.0
21	35.50942614040702	37.0	37.0	37.0	33.0	37.0
22	35.41571027902348	37.0	37.0	37.0	33.0	37.0
23	35.462344726263154	37.0	37.0	37.0	33.0	37.0
24	35.45194565638643	37.0	37.0	37.0	33.0	37.0
25	35.468590109743204	37.0	37.0	37.0	33.0	37.0
26	35.355404856140126	37.0	37.0	37.0	33.0	37.0
27	35.378879395906	37.0	37.0	37.0	33.0	37.0
28	35.390164297472005	37.0	37.0	37.0	33.0	37.0
29	35.403588158482854	37.0	37.0	37.0	33.0	37.0
30	35.40247141639896	37.0	37.0	37.0	33.0	37.0
31	35.385957947301115	37.0	37.0	37.0	33.0	37.0
32	35.3684155090764	37.0	37.0	37.0	33.0	37.0
33	35.360118939108524	37.0	37.0	37.0	33.0	37.0
34	35.33409628288279	37.0	37.0	37.0	33.0	37.0
35	35.284497810402314	37.0	37.0	37.0	33.0	37.0
36	35.311403597610955	37.0	37.0	37.0	33.0	37.0
37	35.30769687811847	37.0	37.0	37.0	33.0	37.0
38	35.28890131213819	37.0	37.0	37.0	33.0	37.0
39	35.18067725613634	37.0	37.0	37.0	33.0	37.0
40	34.97472949049824	37.0	37.0	37.0	27.0	37.0
41	35.14670507071124	37.0	37.0	37.0	33.0	37.0
42	35.17712717761331	37.0	37.0	37.0	33.0	37.0
43	34.841434777265995	37.0	37.0	37.0	27.0	37.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
14	4.0
15	24.0
16	36.0
17	36.0
18	29.0
19	31.0
20	62.0
21	161.0
22	423.0
23	981.0
24	1848.0
25	3415.0
26	5444.0
27	8069.0
28	11156.0
29	15027.0
30	19544.0
31	24367.0
32	30792.0
33	40282.0
34	57932.0
35	115497.0
36	405387.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	40.96228868660598	20.713202538123845	13.791022041815037	24.533486733455135
2	15.916748025446056	21.49303150238945	38.76377866631017	23.82644180585432
3	17.624674733676592	27.403932498544993	29.906677091393252	25.06471567638516
4	12.321027564759563	17.903792736990358	39.65150085004733	30.123678848202744
5	13.777923615921745	37.061118335500645	35.44312514938282	13.717832899194784
6	29.342634566070757	40.12682517112351	17.294918485929994	13.235621776875742
7	26.41763453231193	32.38795106860199	23.703964771986115	17.49044962709997
8	24.650427319265354	35.80150888464878	20.517536361635386	19.03052743445048
9	25.90639081651806	15.37971256382107	20.476080518859707	38.23781610080116
10	14.979737950460942	28.16040035271225	33.85348938014738	23.00637231667943
11	33.74276041898759	23.771077325274426	24.290423160177543	18.19573909556044
12	22.901044768259137	25.88140928259786	30.344731664566865	20.87281428457613
13	27.86521314649847	22.31445134474922	26.262613986688216	23.557721522064096
14	21.893681292342013	21.10331957323438	28.074247819517197	28.928751314906414
15	23.829547618179532	28.651523806051472	24.82826883371346	22.690659742055537
16	22.66891905577904	28.55996986011691	25.41823814018557	23.35287294391848
17	22.264893382864287	27.667386404914208	26.58791406892473	23.47980614329678
18	22.885785777270044	27.24148501040447	27.923413368766603	21.949315843558885
19	24.473936158002125	26.409262342565697	27.82105659735304	21.29574490207914
20	23.835219101556014	26.283679496372276	28.042649554991105	21.838451847080602
21	22.725903960180784	27.051355281973997	28.031846729512104	22.190894028333112
22	22.584657017042808	26.805320931689685	27.888709291915298	22.72131275935221
23	22.441384544127516	26.591019881249938	28.412916398283972	22.55467917633857
24	22.6682438791866	27.29333857270369	27.19152194256408	22.84689560554563
25	22.858643678254047	26.61762183899199	28.155269010609725	22.368465472144237
26	22.753991306426197	27.13561732071023	27.86143215758082	22.248959215282756
27	22.784914394359845	27.110365716153062	27.46537356845683	22.639346321030267
28	22.422344564220772	26.83408345452753	28.111787638056736	22.631784343194962
29	23.491554216005195	26.770076713564432	27.41352000615761	22.32484906427276
30	23.180432842209882	26.546998367422997	27.84576806063626	22.42680072973086
31	22.84419489917588	26.69148615820468	27.927734498958202	22.53658444366124
32	22.229784200057527	26.52876859942718	28.091262269646627	23.15018493086867
33	22.014132796432907	26.724839881871105	28.605476762447218	22.655550559248773
34	22.863504949719598	26.386306338422816	28.130017406052556	22.620171305805034
35	22.587087652775583	27.098617643444644	27.68926212650919	22.625032577270584
36	23.334913246559637	26.834893666438457	27.544369229772048	22.285823857229857
37	22.742378269036266	26.42560161610269	27.724641379952924	23.107378734908117
38	22.78896545391447	26.92023598772259	27.556387373117442	22.734411185245502
39	22.429366400782126	26.76643075996527	27.830509069647164	22.97369376960544
40	22.58519715831676	27.21069695778931	27.981613591034737	22.222492292859197
41	22.538339902801578	26.42452133355479	28.04048898989531	22.996649773748324
42	22.357797681983723	27.159113466127067	27.90531863608927	22.57777021579994
43	22.937774374887752	26.38158010227575	27.64618585991166	23.03445966292484
>>END_MODULE
>>Per sequence GC content	pass
#GC Content	Count
0	30.0
1	46.5
2	63.0
3	265.0
4	467.0
5	467.0
6	556.0
7	645.0
8	591.0
9	537.0
10	708.0
11	879.0
12	879.0
13	1472.0
14	2065.0
15	3719.5
16	5374.0
17	6147.0
18	6920.0
19	6920.0
20	6475.0
21	6030.0
22	6410.0
23	6790.0
24	8522.0
25	10254.0
26	10254.0
27	13075.5
28	15897.0
29	18799.5
30	21702.0
31	25677.5
32	29653.0
33	29653.0
34	34905.5
35	40158.0
36	43094.5
37	46031.0
38	49083.5
39	52136.0
40	52136.0
41	54353.0
42	56570.0
43	57841.0
44	59112.0
45	58103.0
46	57094.0
47	57094.0
48	55378.0
49	53662.0
50	53643.0
51	53624.0
52	50680.5
53	47737.0
54	47737.0
55	45155.5
56	42574.0
57	37651.0
58	32728.0
59	30352.5
60	27977.0
61	27977.0
62	24802.0
63	21627.0
64	17948.0
65	14269.0
66	12337.5
67	10406.0
68	10406.0
69	8688.5
70	6971.0
71	5741.5
72	4512.0
73	3522.5
74	2533.0
75	2533.0
76	2010.5
77	1488.0
78	1169.5
79	851.0
80	681.5
81	512.0
82	512.0
83	411.5
84	311.0
85	253.5
86	196.0
87	146.5
88	97.0
89	97.0
90	73.0
91	49.0
92	30.0
93	11.0
94	8.0
95	5.0
96	5.0
97	2.5
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
43	740547.0
>>END_MODULE
>>Sequence Duplication Levels	warn
#Total Deduplicated Percentage	59.04735156489534
#Duplication Level	Percentage of deduplicated	Percentage of total
1	84.48843598958892	49.88818383045413
2	8.604191354809615	10.161094237181528
3	2.539395688672836	4.498337699743333
4	1.2035261256568686	2.8426012103679015
5	0.6905788769826885	2.038842686624371
6	0.4477255318656986	1.586220413079219
7	0.31842482980496	1.3161500010739802
8	0.2470785330953632	1.1671466406256432
9	0.17116986476052862	0.9096414463647472
>10	1.1197193175314977	12.234852759986001
>50	0.10337891919511216	4.233505832583163
>100	0.06313716471733172	7.011116356591073
>500	0.0025439883217623217	0.9684820552575704
>1k	6.938149968442696E-4	1.1438248300673166
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT	3957	0.534334755255237	No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT	2844	0.3840404457785934	No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT	1575	0.2126806266178919	No Hit
GTATTAGAGGCACCGCCTGCCCAGTGACACATGTTTAACGGCC	762	0.10289691268751341	No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	761	0.10276187736902587	No Hit
GTACATGCTAAGACTTCACCAGTCAAAGCGAACTACTATACTC	743	0.10033124163624996	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	1.3503531848755042E-4	0.0	0.0
3	0.0	0.0	1.3503531848755042E-4	0.0	0.0
4	0.0	0.0	1.3503531848755042E-4	1.3503531848755042E-4	0.0
5	0.0	0.0	4.051059554626513E-4	1.3503531848755042E-4	0.0
6	0.0	0.0	0.0012153178663879538	1.3503531848755042E-4	0.0
7	0.0	0.0	0.0037809889176514118	1.3503531848755042E-4	0.0
8	0.0	0.0	0.004456165510089163	1.3503531848755042E-4	0.0
9	0.0	0.0	0.005536448057989567	8.102119109253026E-4	0.0
10	0.0	0.0	0.0059415540134522184	8.102119109253026E-4	0.0
11	0.0	0.0	0.007967083790765475	8.102119109253026E-4	0.0
12	0.0	0.0	0.008777295701690778	8.102119109253026E-4	0.0
13	0.0	0.0	0.00972254293110363	8.102119109253026E-4	0.0
14	0.0	0.0	0.010532754842028933	8.102119109253026E-4	0.0
15	1.3503531848755042E-4	0.0	0.011478002071441786	8.102119109253026E-4	0.0
16	1.3503531848755042E-4	0.0	0.01255828461934219	8.102119109253026E-4	0.0
17	1.3503531848755042E-4	0.0	0.01282835525631729	9.452472294128529E-4	0.0
18	1.3503531848755042E-4	0.0	0.01296339057480484	9.452472294128529E-4	0.0
19	1.3503531848755042E-4	0.0	0.01296339057480484	9.452472294128529E-4	0.0
20	1.3503531848755042E-4	0.0	0.013638567167242592	0.0010802825479004033	0.0
21	1.3503531848755042E-4	0.0	0.014313743759680345	0.0010802825479004033	0.0
22	1.3503531848755042E-4	0.0	0.014313743759680345	0.0018904944588257059	0.0
23	2.7007063697510083E-4	0.0	0.014448779078167894	0.002295600414288357	0.0
24	2.7007063697510083E-4	0.0	0.014448779078167894	0.0029707770067261092	0.0
25	2.7007063697510083E-4	0.0	0.014448779078167894	0.0029707770067261092	0.0
26	2.7007063697510083E-4	0.0	0.014448779078167894	0.0031058123252136597	0.0
27	2.7007063697510083E-4	0.0	0.014448779078167894	0.003916024236138962	0.0
28	2.7007063697510083E-4	0.0	0.014448779078167894	0.012018143345391988	0.0
29	2.7007063697510083E-4	0.0	0.014448779078167894	0.029437699430285992	0.0
30	2.7007063697510083E-4	0.0	0.014448779078167894	0.05171852698073181	0.0
31	2.7007063697510083E-4	0.0	0.014448779078167894	0.11342966752954235	0.0
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GTATCAA	1715	0.0	23.83965	1
CTAGACA	75	3.737041E-7	22.2	4
CGGGACT	95	7.1340764E-9	21.421053	17
TAGGACC	190	0.0	21.421053	4
GGTATCA	625	0.0	21.016	1
TTTACCG	45	0.0038245427	20.555555	30
TATTACG	45	0.0038245427	20.555555	2
GCCGGCA	145	0.0	20.413794	15
TTCGCCG	155	0.0	20.290323	24
AGCTTCG	185	0.0	19.000002	21
GCTTCGC	185	0.0	19.000002	22
AGGACCG	80	1.6154047E-5	18.5	5
GCCGCTC	170	0.0	18.5	27
CCGAGTT	70	1.2184538E-4	18.5	13
CGACACG	50	0.007032578	18.499998	21
ACTAGAC	50	0.007032578	18.499998	3
GTACTAG	50	0.007032578	18.499998	1
TGTACGA	50	0.007032578	18.499998	2
TTAACGG	205	0.0	18.048782	35
TAACGGC	210	0.0	17.619047	36
>>END_MODULE