FastQCFastQC Report
Fri 10 Feb 2017
ERR1633276.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1633276.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences741956
Sequences flagged as poor quality0
Sequence length43
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT100801.3585711282070634No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT73230.9869857511766197No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT38660.5210551569095742No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT20090.27077077346904666No Hit
ATCATTAACTGAATCCATAGGTTAATGAGGCGAACCGGGGGAA12650.17049528543471582No Hit
GTATGAAACTGCTCGCCGGCAGCTTCGCCGCTCTCTTCCTGAG11470.1545913773862601No Hit
GTACTGGTTCACTATCGGTCAGTCAGGAGTATTTAGCCTTGGA11190.15081756869679605No Hit
CCGTATTACCGCGGCTGCTGGCACGGAGTTAGCCGGTGCTTCT10750.1448872978990668No Hit
ATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTT10420.14043959480076984No Hit
GTTAATGATAGTGTGTCGAAACACACTGGGTTTCCCCATTCGG9370.12628781221527963No Hit
GTTAATACCTTTGCTCATTGACGTTACCCGCAGAAGAAGCACC8650.11658373272808631No Hit
ATTGAAAGCTGAGTATTTTTAAGACAAAGGTTTCAGGAAGAAA8560.11537072279218714No Hit
ATACAGGGTGACAGCCCCGTACACAAAAATGCACATGCTGTGA8430.11361859732922168No Hit
GTATGAAACTGCTTGTCCGGCGAGTCGCCGTCCGCCGCCTGGG7480.10081460356139718No Hit
TCTTTAGTGACCATGAAGTGCGATTGCCTCGGTGATAACGACA7460.10054504579786402No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ACAACGC200.001841515837.03
CGTTATG250.00549510429.5999982
GGTATCA15300.025.2712421
GCCGCTC2900.023.60344927
TTCGCCG2900.023.60344924
CGCTCTC2950.023.2033929
GTATCAA41600.022.9915871
CCGCTCT3050.022.44262328
GCCGGCA3050.021.83606515
CGTTTAG705.0979015E-621.14285726
TGCTCGC3600.020.55555310
ACCGTAT450.0038245520.5555538
CTGCTCG4100.020.304889
CCGGCAG3200.020.23437516
AGCTTCG3200.020.23437521
CGGGCCA1403.6379788E-1219.82142817
TCCGCCG2250.019.73333431
CGGCAGC3200.019.07812517
GCCGTCC2350.018.89361827
GCTTCGC3450.018.76811622
Produced by FastQC (version 0.11.5)