##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	ERR1633276.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	741956
Sequences flagged as poor quality	0
Sequence length	43
%GC	44
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.812333885028224	33.0	33.0	33.0	27.0	33.0
2	31.932855856681527	33.0	33.0	33.0	33.0	33.0
3	31.66320644350878	33.0	33.0	33.0	27.0	33.0
4	31.937065001159098	33.0	33.0	33.0	33.0	33.0
5	32.039387780407466	33.0	33.0	33.0	33.0	33.0
6	35.17212206653764	37.0	37.0	37.0	33.0	37.0
7	35.330000700850185	37.0	37.0	37.0	33.0	37.0
8	35.477673878235365	37.0	37.0	37.0	33.0	37.0
9	35.596249642835964	37.0	37.0	37.0	33.0	37.0
10	35.57057830922589	37.0	37.0	37.0	33.0	37.0
11	35.596357465941374	37.0	37.0	37.0	33.0	37.0
12	35.593488023548566	37.0	37.0	37.0	33.0	37.0
13	35.59609060375548	37.0	37.0	37.0	33.0	37.0
14	35.576191849651465	37.0	37.0	37.0	33.0	37.0
15	35.60287672045243	37.0	37.0	37.0	33.0	37.0
16	35.58271110416251	37.0	37.0	37.0	33.0	37.0
17	35.58448883761301	37.0	37.0	37.0	33.0	37.0
18	35.56125834955173	37.0	37.0	37.0	33.0	37.0
19	35.55122810517066	37.0	37.0	37.0	33.0	37.0
20	35.559902474001156	37.0	37.0	37.0	33.0	37.0
21	35.544462205305976	37.0	37.0	37.0	33.0	37.0
22	35.458994873011335	37.0	37.0	37.0	33.0	37.0
23	35.50016847360221	37.0	37.0	37.0	33.0	37.0
24	35.48460959949107	37.0	37.0	37.0	33.0	37.0
25	35.508833407910984	37.0	37.0	37.0	33.0	37.0
26	35.403588622505914	37.0	37.0	37.0	33.0	37.0
27	35.412998884030856	37.0	37.0	37.0	33.0	37.0
28	35.40700661494752	37.0	37.0	37.0	33.0	37.0
29	35.420472373024815	37.0	37.0	37.0	33.0	37.0
30	35.41828760735138	37.0	37.0	37.0	33.0	37.0
31	35.40768590051162	37.0	37.0	37.0	33.0	37.0
32	35.375864606526534	37.0	37.0	37.0	33.0	37.0
33	35.361699346052866	37.0	37.0	37.0	33.0	37.0
34	35.31185272442032	37.0	37.0	37.0	33.0	37.0
35	35.23400174673431	37.0	37.0	37.0	33.0	37.0
36	35.23576869787427	37.0	37.0	37.0	33.0	37.0
37	35.2181396201392	37.0	37.0	37.0	33.0	37.0
38	35.1784054580056	37.0	37.0	37.0	33.0	37.0
39	35.055674460480134	37.0	37.0	37.0	33.0	37.0
40	34.812440360344816	37.0	37.0	37.0	27.0	37.0
41	34.94527977400277	37.0	37.0	37.0	27.0	37.0
42	34.946360700634536	37.0	37.0	37.0	27.0	37.0
43	34.58624500644243	37.0	37.0	37.0	27.0	37.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
14	2.0
15	28.0
16	55.0
17	37.0
18	32.0
19	33.0
20	41.0
21	158.0
22	370.0
23	932.0
24	1747.0
25	3313.0
26	5249.0
27	7914.0
28	11083.0
29	14872.0
30	19508.0
31	25327.0
32	32038.0
33	41208.0
34	58231.0
35	114704.0
36	405074.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	42.92087401409248	20.120734922286495	14.458269762627435	22.500121300993587
2	15.545800559601917	23.324024605232655	39.63536921326871	21.494805621896717
3	18.16751936772531	28.521367843915275	31.339324703890796	21.97178808446862
4	11.869841338300384	18.06171794553855	40.751473133177704	29.316967582983356
5	13.626953619891207	37.253287256926285	34.1388168570643	14.980942266118205
6	27.077616462431735	41.5369644561133	17.18646388734642	14.198955194108546
7	24.67626112599669	34.095013720490165	23.060397112497235	18.16832804101591
8	24.818048509615124	34.110648070775085	20.951107612850357	20.12019580675943
9	26.98812328493873	14.568384109030724	20.46442107079126	37.979071535239285
10	15.020297699594046	28.10018383839473	33.15533535681361	23.724183105197614
11	32.93295020189876	24.72707276442269	23.984171568125333	18.355805465553214
12	23.090318024249417	26.80644135231739	30.53253831763609	19.57070230579711
13	29.324919537007588	23.162155168231	26.361940600251227	21.150984694510186
14	22.539881071114728	22.204551213279494	29.79772385424473	25.457843861361052
15	24.1794661678051	28.27054434494768	26.695248774859966	20.854740712387258
16	20.708101289025223	29.326536883588783	26.588234342737305	23.377127484648685
17	21.388599863064655	27.079772924540002	27.21738216282367	24.314245049571674
18	23.388583689598843	25.035177288141075	29.75769452636005	21.818544495900028
19	24.759554474928432	25.4806214923796	30.373768794915062	19.386055237776905
20	24.887729191488443	24.804840179202	29.65431912404509	20.653111505264462
21	22.492304125851128	26.83973173611373	29.586929683161806	21.081034454873336
22	21.961679668336128	26.57071308810765	29.951641337222153	21.51596590633407
23	21.803179703378635	26.07337901438899	30.60761015478007	21.5158311274523
24	22.00602192043733	26.20330585641197	30.35018249060591	21.440489732544787
25	22.079611189881877	26.37703583500908	30.705324844060833	20.838028131048205
26	22.076915612246548	26.485937171476476	29.904873065249156	21.532274151027824
27	22.120718748820682	26.498875944126066	30.196669344273786	21.183735962779465
28	21.392508450635887	26.73285208287284	30.029273973119704	21.845365493371574
29	22.069772331512922	26.589582131554973	29.53719627578994	21.80344926114217
30	22.183390928842144	26.661014938891253	30.135614510833523	21.019979621433077
31	22.245389214454768	26.37919229711735	29.903525276431488	21.4718932119964
32	20.82562847392568	26.51531896770159	30.29478837019985	22.364264188172882
33	21.045991945614027	26.458037942950796	30.80896980413933	21.68700030729585
34	21.223360954018837	26.49901072300783	29.926572465213567	22.35105585775976
35	21.295063319118654	27.259702731698376	30.00258775452992	21.442646194653054
36	21.738081503485382	26.737030228207605	30.08278118918103	21.442107079125986
37	22.308061394476223	26.661419275536552	29.450803012577566	21.57971631740966
38	21.878790656049684	27.134762708300762	29.28246418925112	21.70398244639844
39	21.7073519184426	26.39927435050057	29.676692418418344	22.216681312638485
40	21.69926518553661	26.944185369482827	29.928055032913004	21.42849441206756
41	21.929063178948617	26.443481823720006	29.79125446791993	21.83620052941145
42	21.069308692159645	27.045269530807758	29.959458512364616	21.925963264667985
43	21.228212993762433	27.007261886149582	29.552291510547796	22.21223360954019
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	116.0
1	139.0
2	162.0
3	677.5
4	1193.0
5	1193.0
6	1371.0
7	1549.0
8	1478.0
9	1407.0
10	2005.0
11	2603.0
12	2603.0
13	4121.0
14	5639.0
15	9623.0
16	13607.0
17	14919.5
18	16232.0
19	16232.0
20	14178.0
21	12124.0
22	11711.0
23	11298.0
24	13537.5
25	15777.0
26	15777.0
27	17660.0
28	19543.0
29	22992.0
30	26441.0
31	28101.0
32	29761.0
33	29761.0
34	31844.0
35	33927.0
36	35870.0
37	37813.0
38	38956.5
39	40100.0
40	40100.0
41	42581.5
42	45063.0
43	45566.0
44	46069.0
45	48474.5
46	50880.0
47	50880.0
48	52084.5
49	53289.0
50	54926.5
51	56564.0
52	55911.0
53	55258.0
54	55258.0
55	50094.0
56	44930.0
57	40668.0
58	36406.0
59	32842.5
60	29279.0
61	29279.0
62	24676.0
63	20073.0
64	15857.0
65	11641.0
66	10257.5
67	8874.0
68	8874.0
69	7184.5
70	5495.0
71	4632.0
72	3769.0
73	3155.0
74	2541.0
75	2541.0
76	1767.0
77	993.0
78	748.0
79	503.0
80	406.0
81	309.0
82	309.0
83	276.5
84	244.0
85	227.5
86	211.0
87	183.5
88	156.0
89	156.0
90	123.0
91	90.0
92	56.0
93	22.0
94	13.5
95	5.0
96	5.0
97	2.5
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
43	741956.0
>>END_MODULE
>>Sequence Duplication Levels	warn
#Total Deduplicated Percentage	56.436717816177364
#Duplication Level	Percentage of deduplicated	Percentage of total
1	88.01538478737628	49.67299434727425
2	6.9465899257142	7.840854708444656
3	1.6786607279495633	2.8421430543716513
4	0.7436888505797538	1.6788543121282744
5	0.45413695209827337	1.2814999507734555
6	0.3033970219956928	1.0273639269983683
7	0.23448923019122875	0.9263661760664526
8	0.17870494464421258	0.8068416426592817
9	0.12821781700419344	0.651257348154474
>10	1.0850806432877875	12.579502941545487
>50	0.13670703391204306	5.404817038419542
>100	0.08741468922985518	9.343719055448368
>500	0.005342008786240011	1.9798000849722188
>1k	0.0016997300683490946	1.5790985242012099
>5k	2.4281858119272782E-4	1.0035354068146713
>10k+	2.4281858119272782E-4	1.381351481727692
>>END_MODULE
>>Overrepresented sequences	fail
#Sequence	Count	Percentage	Possible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT	10080	1.3585711282070634	No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT	7323	0.9869857511766197	No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT	3866	0.5210551569095742	No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	2009	0.27077077346904666	No Hit
ATCATTAACTGAATCCATAGGTTAATGAGGCGAACCGGGGGAA	1265	0.17049528543471582	No Hit
GTATGAAACTGCTCGCCGGCAGCTTCGCCGCTCTCTTCCTGAG	1147	0.1545913773862601	No Hit
GTACTGGTTCACTATCGGTCAGTCAGGAGTATTTAGCCTTGGA	1119	0.15081756869679605	No Hit
CCGTATTACCGCGGCTGCTGGCACGGAGTTAGCCGGTGCTTCT	1075	0.1448872978990668	No Hit
ATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTT	1042	0.14043959480076984	No Hit
GTTAATGATAGTGTGTCGAAACACACTGGGTTTCCCCATTCGG	937	0.12628781221527963	No Hit
GTTAATACCTTTGCTCATTGACGTTACCCGCAGAAGAAGCACC	865	0.11658373272808631	No Hit
ATTGAAAGCTGAGTATTTTTAAGACAAAGGTTTCAGGAAGAAA	856	0.11537072279218714	No Hit
ATACAGGGTGACAGCCCCGTACACAAAAATGCACATGCTGTGA	843	0.11361859732922168	No Hit
GTATGAAACTGCTTGTCCGGCGAGTCGCCGTCCGCCGCCTGGG	748	0.10081460356139718	No Hit
TCTTTAGTGACCATGAAGTGCGATTGCCTCGGTGATAACGACA	746	0.10054504579786402	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	6.738944088328688E-4	0.0	0.0
2	0.0	0.0	6.738944088328688E-4	0.0	0.0
3	0.0	0.0	6.738944088328688E-4	1.3477888176657377E-4	0.0
4	0.0	0.0	8.086732905994426E-4	1.3477888176657377E-4	0.0
5	0.0	0.0	0.0016173465811988852	1.3477888176657377E-4	0.0
6	1.3477888176657377E-4	0.0	0.003369472044164344	1.3477888176657377E-4	0.0
7	1.3477888176657377E-4	0.0	0.007547617378928131	2.6955776353314754E-4	0.0
8	2.6955776353314754E-4	0.0	0.008895406196593868	4.043366452997213E-4	0.0
9	2.6955776353314754E-4	0.0	0.011186647186625622	0.0013477888176657376	0.0
10	8.086732905994426E-4	0.0	0.012938772649591082	0.0014825676994323113	0.0
11	9.434521723660163E-4	0.0	0.019542937856153197	0.001752125462965459	0.0
12	9.434521723660163E-4	0.0	0.021968957727951523	0.001752125462965459	0.0
13	9.434521723660163E-4	0.0	0.023451525427383834	0.0018869043447320326	0.0
14	9.434521723660163E-4	0.0	0.025877545299182163	0.0020216832264986066	0.0
15	9.434521723660163E-4	0.0	0.029112238461579933	0.0024260198717983277	0.0
16	9.434521723660163E-4	0.0	0.031807816096911405	0.0025607987535649014	0.0
17	9.434521723660163E-4	0.0	0.03248171050574428	0.002695577635331475	0.0
18	9.434521723660163E-4	0.0	0.03342516267811029	0.0032346931623977704	0.0
19	9.434521723660163E-4	0.0	0.034368614850476306	0.003369472044164344	0.0
20	9.434521723660163E-4	0.0	0.03531206702284233	0.0036390298076974916	0.0
21	9.434521723660163E-4	0.0	0.03692941360404121	0.004447703098296934	0.0
22	9.434521723660163E-4	0.0	0.037064192485807786	0.005256376388896377	0.0
23	9.434521723660163E-4	0.0	0.037064192485807786	0.007008501851861836	0.0
24	0.0010782310541325901	0.0	0.03719897136757436	0.007817175142461278	0.0
25	0.0010782310541325901	0.0	0.03719897136757436	0.008221511787761	0.0
26	0.0010782310541325901	0.0	0.03719897136757436	0.009704079487193311	0.0
27	0.0010782310541325901	0.0	0.03719897136757436	0.013477888176657377	0.0
28	0.0010782310541325901	0.0	0.03733375024934093	0.03382949932341001	0.0
29	0.0012130099358991639	0.0	0.03733375024934093	0.07520661602574816	0.0
30	0.0012130099358991639	0.0	0.03733375024934093	0.1258834755699799	0.0
31	0.0012130099358991639	0.0	0.03733375024934093	0.2254850691954779	0.0
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
ACAACGC	20	0.0018415158	37.0	3
CGTTATG	25	0.005495104	29.599998	2
GGTATCA	1530	0.0	25.271242	1
GCCGCTC	290	0.0	23.603449	27
TTCGCCG	290	0.0	23.603449	24
CGCTCTC	295	0.0	23.20339	29
GTATCAA	4160	0.0	22.991587	1
CCGCTCT	305	0.0	22.442623	28
GCCGGCA	305	0.0	21.836065	15
CGTTTAG	70	5.0979015E-6	21.142857	26
TGCTCGC	360	0.0	20.555553	10
ACCGTAT	45	0.00382455	20.555553	8
CTGCTCG	410	0.0	20.30488	9
CCGGCAG	320	0.0	20.234375	16
AGCTTCG	320	0.0	20.234375	21
CGGGCCA	140	3.6379788E-12	19.821428	17
TCCGCCG	225	0.0	19.733334	31
CGGCAGC	320	0.0	19.078125	17
GCCGTCC	235	0.0	18.893618	27
GCTTCGC	345	0.0	18.768116	22
>>END_MODULE