FastQCFastQC Report
Fri 10 Feb 2017
ERR1633275.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1633275.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences474216
Sequences flagged as poor quality0
Sequence length43
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT22910.48311318049159035No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT17280.36439091047117766No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT10140.21382661065843414No Hit
GTATTAGAGGCACCGCCTGCCCAGTGACACATGTTTAACGGCC5410.11408303389172866No Hit
TTATTACCCTCACTGTCAACCCAACACAGGCATGCTCATAAGG5310.11197429019687231No Hit
GTACATGCTAAGACTTCACCAGTCAAAGCGAACTACTATACTC5250.1107090439799585No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT4870.10269581793950436No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CCGCTCT509.07639E-625.90000228
GCACGGA451.3216115E-424.66666621
CGGAGTT502.6988596E-422.224
CGCTCTC603.7203466E-521.58333429
GACGGGT603.7203466E-521.5833346
GGTATCA4900.021.1428591
CTTCGCC705.0915005E-621.14285723
GCCGGCA806.9423913E-720.812515
TGCGGGT555.1369733E-420.18181821
ACGGGTA555.1369733E-420.18181816
GCCGCTC656.891206E-519.92307727
AGGTTAC656.891206E-519.9230777
GTATCAA11150.019.9103151
TTGGCCG500.007028937318.531
CATGCTA1103.83734E-818.54
TAGACGG701.217282E-418.54
GTTTAGG902.1467968E-618.51
AGCTTCG801.6134089E-518.521
TCGGGAT801.6134089E-518.522
CCGTACA1002.8660907E-718.517
Produced by FastQC (version 0.11.5)