FastQCFastQC Report
Fri 10 Feb 2017
ERR1633274.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1633274.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences431303
Sequences flagged as poor quality0
Sequence length43
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT18940.4391344368112441No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT15700.36401323431555077No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT8400.19475867313698259No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA4150.028.0843371
TCCGCCG358.860376E-426.4285731
CATTAGT451.3213401E-424.66666823
TTCGCCG603.7193415E-521.58333424
TGCTCGC705.089736E-621.14285710
GCGGGTA450.00382199120.55555722
GTATCAA10150.020.2315271
AGCTTCG759.243779E-619.73333421
GCCGGCA759.243779E-619.73333415
GCTTCGC759.243779E-619.73333422
GCCGCTC801.6128577E-518.527
CGCTCTC701.21695746E-418.529
CCGGCAG752.0634959E-417.26666616
TCAACCC752.0634959E-417.26666616
GTATATA1201.036733E-716.9583341
GTACCAG1005.860684E-616.651
GTATAAA1005.860684E-616.651
TTATACT1251.650551E-716.2799994
TACTTGT803.3764404E-416.18757
TCGCCGG1059.315978E-615.85714213
Produced by FastQC (version 0.11.5)