##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	ERR1633274.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	431303
Sequences flagged as poor quality	0
Sequence length	43
%GC	44
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	30.832505222546562	33.0	33.0	33.0	27.0	33.0
2	31.2082016586947	33.0	33.0	33.0	27.0	33.0
3	30.964804325497386	33.0	33.0	33.0	27.0	33.0
4	31.284690809013618	33.0	33.0	33.0	27.0	33.0
5	31.39281665093913	33.0	33.0	33.0	27.0	33.0
6	33.74217197654549	37.0	33.0	37.0	27.0	37.0
7	33.9626202460915	37.0	33.0	37.0	27.0	37.0
8	34.113583721884616	37.0	33.0	37.0	27.0	37.0
9	34.16959075174529	37.0	33.0	37.0	27.0	37.0
10	34.18917559117372	37.0	33.0	37.0	27.0	37.0
11	34.23181382925693	37.0	33.0	37.0	27.0	37.0
12	34.27988444318727	37.0	33.0	37.0	27.0	37.0
13	34.2983447831339	37.0	37.0	37.0	27.0	37.0
14	34.296995383755736	37.0	37.0	37.0	27.0	37.0
15	34.286260471176874	37.0	37.0	37.0	27.0	37.0
16	34.26087460555572	37.0	37.0	37.0	27.0	37.0
17	34.32352197874812	37.0	37.0	37.0	27.0	37.0
18	34.272520710498185	37.0	37.0	37.0	27.0	37.0
19	34.24496699536057	37.0	37.0	37.0	27.0	37.0
20	34.26223791626768	37.0	37.0	37.0	27.0	37.0
21	34.26415072466456	37.0	37.0	37.0	27.0	37.0
22	34.14959552796989	37.0	33.0	37.0	27.0	37.0
23	34.18751550534079	37.0	33.0	37.0	27.0	37.0
24	34.1840005750018	37.0	37.0	37.0	27.0	37.0
25	34.196523093973376	37.0	37.0	37.0	27.0	37.0
26	34.070817963241616	37.0	33.0	37.0	27.0	37.0
27	34.06372550156155	37.0	33.0	37.0	27.0	37.0
28	34.07379498867385	37.0	33.0	37.0	27.0	37.0
29	34.09597661041078	37.0	33.0	37.0	27.0	37.0
30	34.080173335219094	37.0	33.0	37.0	27.0	37.0
31	34.08957275975359	37.0	33.0	37.0	27.0	37.0
32	34.052281110959115	37.0	33.0	37.0	27.0	37.0
33	34.04549701717818	37.0	33.0	37.0	27.0	37.0
34	34.01149308027072	37.0	33.0	37.0	27.0	37.0
35	33.9532277772239	37.0	33.0	37.0	27.0	37.0
36	33.97977060210571	37.0	33.0	37.0	27.0	37.0
37	33.99067245069012	37.0	33.0	37.0	27.0	37.0
38	33.96410875880761	37.0	33.0	37.0	27.0	37.0
39	33.84179335641069	37.0	33.0	37.0	27.0	37.0
40	33.63296336913956	37.0	33.0	37.0	27.0	37.0
41	33.83377347247759	37.0	33.0	37.0	27.0	37.0
42	33.87429486926824	37.0	33.0	37.0	27.0	37.0
43	33.41053505308333	37.0	33.0	37.0	22.0	37.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
14	4.0
15	26.0
16	40.0
17	41.0
18	39.0
19	65.0
20	188.0
21	463.0
22	1080.0
23	1953.0
24	3366.0
25	5302.0
26	7245.0
27	9946.0
28	12566.0
29	15887.0
30	19207.0
31	23685.0
32	28930.0
33	37631.0
34	52320.0
35	86967.0
36	124352.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	40.133270577760875	22.16724669200075	13.895103906070675	23.804378824167696
2	15.998729431513345	23.769368634115693	37.82908999009977	22.402811944271196
3	18.37524895491105	28.04200295383988	30.776971178034927	22.805776913214142
4	13.415626601252484	19.623327451930546	38.62551384989207	28.335532096924897
5	14.132292147283929	37.870592135923005	35.17944461318377	12.817671103609296
6	28.236761626976858	40.86987570223254	17.75828130108068	13.135081369709924
7	25.06103597702775	33.328077940566146	24.053159843543867	17.55772623886224
8	23.806001813110505	36.860629302369794	21.08888646728634	18.24448241723336
9	25.556047604584247	15.82182363674725	20.6847622205271	37.9373665381414
10	14.277897441010149	29.18713758077268	34.53813212521128	21.99683285300589
11	32.67888236344287	24.288261384687797	24.961801795953193	18.07105445591614
12	21.743878433491073	26.597310939177333	31.227234681882578	20.431575945449023
13	27.491577846664644	22.790242590475838	27.18529664760041	22.532882915259112
14	21.317032341532517	21.968314618725117	28.71855748742763	27.99609555231473
15	23.253953716992463	29.565989571136765	25.120159145658622	22.059897566212154
16	22.069171788742484	29.40021284340707	25.806451612903224	22.72416375494722
17	21.610793340180802	28.66244844111912	27.166980058103	22.559778160597073
18	22.03833499882913	28.051972743059984	28.508960058242117	21.40073219986877
19	23.751747611308062	27.258794861153294	28.788577867531647	20.200879660007
20	23.457291045970003	27.008390852834317	28.602397850235217	20.931920250960463
21	22.158204325033676	28.027396053354604	27.958303095503624	21.856096526108097
22	21.899685372000658	27.895006526734107	28.05220459862324	22.153103502641994
23	21.77888862354308	27.826609135572905	28.611672072765547	21.78283016811847
24	22.44871934579634	28.036438420321673	27.770268233701135	21.744574000180847
25	22.464949235224424	27.514995258553732	28.50061325796482	21.519442248257025
26	22.31470683023304	27.774209778276525	28.03551099806864	21.875572393421795
27	22.160986591792778	27.701870842539932	27.853968092037384	22.283174473629906
28	21.55445243830903	27.988676174290468	28.530986336751656	21.925885050648848
29	22.603598862052895	27.928625583406564	27.804582857063366	21.66319269747718
30	22.162377725172327	28.011398019489782	28.135672601396234	21.69055165394166
31	22.41440472243411	27.52334205883103	28.316056229611203	21.746196989123657
32	21.647426519175614	27.585247494221	28.440562667080915	22.32676331952247
33	21.687073820492785	27.50201134701127	28.713920376162466	22.096994456333483
34	21.778193056853304	27.63486458475828	28.303304173631993	22.283638184756423
35	21.9022357831965	28.14680166843263	28.138686723718592	21.812275824652275
36	22.381017521324914	27.95737567325059	27.825913568883127	21.835693236541363
37	22.70028263193161	27.246274660737345	27.991458441049566	22.061984266281478
38	22.4684270686733	27.88318189300793	27.955984539871043	21.692406498447728
39	22.13896031328324	27.603332228155146	28.10877735605827	22.148930102503346
40	22.04900035473901	27.78023802292124	28.304231595885028	21.86653002645472
41	22.05873828839586	27.631154895746146	28.17787031390925	22.132236501948746
42	22.04900035473901	27.623503662158623	27.992385863302598	22.33511011979977
43	22.159363602849968	27.417615921985238	28.09486602226277	22.328154452902023
>>END_MODULE
>>Per sequence GC content	pass
#GC Content	Count
0	16.0
1	18.0
2	20.0
3	94.5
4	169.0
5	169.0
6	219.5
7	270.0
8	280.0
9	290.0
10	425.5
11	561.0
12	561.0
13	855.0
14	1149.0
15	2214.5
16	3280.0
17	3707.5
18	4135.0
19	4135.0
20	4280.0
21	4425.0
22	5173.5
23	5922.0
24	7303.5
25	8685.0
26	8685.0
27	10609.0
28	12533.0
29	15063.5
30	17594.0
31	19034.0
32	20474.0
33	20474.0
34	22557.5
35	24641.0
36	26150.0
37	27659.0
38	29374.0
39	31089.0
40	31089.0
41	32121.0
42	33153.0
43	32080.5
44	31008.0
45	31044.5
46	31081.0
47	31081.0
48	30181.0
49	29281.0
50	29763.5
51	30246.0
52	29716.5
53	29187.0
54	29187.0
55	26171.5
56	23156.0
57	20511.0
58	17866.0
59	15815.0
60	13764.0
61	13764.0
62	12067.0
63	10370.0
64	8521.0
65	6672.0
66	5688.5
67	4705.0
68	4705.0
69	3857.0
70	3009.0
71	2502.5
72	1996.0
73	1681.5
74	1367.0
75	1367.0
76	1002.5
77	638.0
78	520.5
79	403.0
80	313.0
81	223.0
82	223.0
83	164.5
84	106.0
85	98.0
86	90.0
87	66.5
88	43.0
89	43.0
90	30.5
91	18.0
92	12.5
93	7.0
94	4.0
95	1.0
96	1.0
97	0.5
98	0.0
99	0.5
100	1.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
43	431303.0
>>END_MODULE
>>Sequence Duplication Levels	warn
#Total Deduplicated Percentage	67.32736519137443
#Duplication Level	Percentage of deduplicated	Percentage of total
1	90.22759712102112	60.747863817071924
2	5.623521239336032	7.572337362844552
3	1.3941944855920014	2.8160232383775923
4	0.6527255749080937	1.757851726063482
5	0.3896662458716419	1.3117600819275967
6	0.2663896199030201	1.0761186733441235
7	0.17413896028774778	0.8207022161337609
8	0.14441265624828198	0.7778338916387599
9	0.11327795119824395	0.6864035389609375
>10	0.8644275328919546	11.913540475234575
>50	0.10569587334829643	4.883186244320341
>100	0.04290624559853441	4.6255460297409385
>500	3.488312650287351E-4	0.19728147575437363
>1k	6.976625300574702E-4	0.8135512285870836
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT	1894	0.4391344368112441	No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT	1570	0.36401323431555077	No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT	840	0.19475867313698259	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	6.955666897749378E-4	0.0	0.0
2	0.0	0.0	6.955666897749378E-4	0.0	0.0
3	0.0	0.0	9.274222530332504E-4	0.0	0.0
4	0.0	0.0	9.274222530332504E-4	2.318555632583126E-4	0.0
5	0.0	0.0	0.0013911333795498756	9.274222530332504E-4	0.0
6	0.0	0.0	0.0020867000693248136	0.001159277816291563	0.0
7	0.0	0.0	0.0032459778856163764	0.001159277816291563	0.0
8	0.0	0.0	0.0032459778856163764	0.001159277816291563	0.0
9	0.0	0.0	0.003941544575391314	0.002318555632583126	0.0
10	4.637111265166252E-4	0.0	0.00533267795494119	0.002550411195841439	0.0
11	4.637111265166252E-4	0.0	0.009506078093590817	0.002550411195841439	0.0
12	6.955666897749378E-4	0.0	0.009969789220107442	0.002550411195841439	0.0
13	6.955666897749378E-4	0.0	0.010897211473140692	0.002550411195841439	0.0
14	6.955666897749378E-4	0.0	0.011824633726173943	0.002550411195841439	0.0
15	6.955666897749378E-4	0.0	0.013447622668982131	0.002550411195841439	0.0
16	6.955666897749378E-4	0.0	0.014143189358757068	0.002550411195841439	0.0
17	6.955666897749378E-4	0.0	0.01437504492201538	0.002550411195841439	0.0
18	6.955666897749378E-4	0.0	0.01437504492201538	0.002782266759099751	0.0
19	6.955666897749378E-4	0.0	0.014838756048532006	0.003014122322358064	0.0
20	6.955666897749378E-4	0.0	0.015302467175048632	0.003014122322358064	0.0
21	6.955666897749378E-4	0.0	0.015302467175048632	0.0032459778856163764	0.0
22	6.955666897749378E-4	0.0	0.015766178301565258	0.0032459778856163764	0.0
23	6.955666897749378E-4	0.0	0.015766178301565258	0.003477833448874689	0.0
24	6.955666897749378E-4	0.0	0.015766178301565258	0.004173400138649627	0.0
25	6.955666897749378E-4	0.0	0.01599803386482357	0.004173400138649627	0.0
26	6.955666897749378E-4	0.0	0.01599803386482357	0.004637111265166252	0.0
27	6.955666897749378E-4	0.0	0.01599803386482357	0.006028244644716128	0.0
28	6.955666897749378E-4	0.0	0.01599803386482357	0.01252020041594888	0.0
29	9.274222530332504E-4	0.0	0.01599803386482357	0.02504040083189776	0.0
30	9.274222530332504E-4	0.0	0.01599803386482357	0.042893279202787835	0.0
31	9.274222530332504E-4	0.0	0.01599803386482357	0.09691562544197467	0.0
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GGTATCA	415	0.0	28.084337	1
TCCGCCG	35	8.860376E-4	26.42857	31
CATTAGT	45	1.3213401E-4	24.666668	23
TTCGCCG	60	3.7193415E-5	21.583334	24
TGCTCGC	70	5.089736E-6	21.142857	10
GCGGGTA	45	0.003821991	20.555557	22
GTATCAA	1015	0.0	20.231527	1
AGCTTCG	75	9.243779E-6	19.733334	21
GCCGGCA	75	9.243779E-6	19.733334	15
GCTTCGC	75	9.243779E-6	19.733334	22
GCCGCTC	80	1.6128577E-5	18.5	27
CGCTCTC	70	1.21695746E-4	18.5	29
CCGGCAG	75	2.0634959E-4	17.266666	16
TCAACCC	75	2.0634959E-4	17.266666	16
GTATATA	120	1.036733E-7	16.958334	1
GTACCAG	100	5.860684E-6	16.65	1
GTATAAA	100	5.860684E-6	16.65	1
TTATACT	125	1.650551E-7	16.279999	4
TACTTGT	80	3.3764404E-4	16.1875	7
TCGCCGG	105	9.315978E-6	15.857142	13
>>END_MODULE