FastQCFastQC Report
Fri 10 Feb 2017
ERR1633273.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1633273.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences785591
Sequences flagged as poor quality0
Sequence length43
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT88811.130486474514092No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT64470.820656041120634No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT34150.43470457273568563No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT16120.2051958334553222No Hit
ATCATTAACTGAATCCATAGGTTAATGAGGCGAACCGGGGGAA11140.1418040685292983No Hit
GTATGAAACTGCTCGCCGGCAGCTTCGCCGCTCTCTTCCTGAG11010.14014926342078765No Hit
GTACTGGTTCACTATCGGTCAGTCAGGAGTATTTAGCCTTGGA9610.12232828532913437No Hit
ATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTT8920.1135450889839624No Hit
CCGTATTACCGCGGCTGCTGGCACGGAGTTAGCCGGTGCTTCT8200.10438001453682642No Hit
GTTAATGATAGTGTGTCGAAACACACTGGGTTTCCCCATTCGG7890.10043394081653177No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTAAAT303.5984511E-430.83333215
ATCGGCG358.866886E-426.4285710
ACGGTAC701.9213076E-723.7857133
GTATCAA36200.022.997241
GGTATCA13500.022.7481481
CACGGTA705.098531E-621.1428572
TTCGCCG2800.021.14285724
GCCGGCA2750.020.85454615
CGGCGTA450.00382474520.55555712
GCTTCGC3150.020.55555522
GATTAAT656.898575E-519.9230771
CGCTCTC2850.019.47368429
CCGCTCT2900.019.1379328
GCCGCTC2900.019.1379327
AGCTTCG3400.019.04411721
CGAGCTC2650.018.84905628
CCGGCAG3150.018.79365216
TCGGCGT500.007032947618.511
ACGGCGT500.007032947618.56
CGGCAGC3250.018.21538517
Produced by FastQC (version 0.11.5)