FastQCFastQC Report
Fri 10 Feb 2017
ERR1633272.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1633272.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences336722
Sequences flagged as poor quality0
Sequence length43
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT14240.4229007905631352No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT12460.37003819174274327No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT7630.22659642078628658No Hit
GTACATGCTAAGACTTCACCAGTCAAAGCGAACTACTATACTC6470.19214663728535705No Hit
AGCATGTACTGCTCGGAGGTTGGGTTCTGCTCCGAGGTCGCCC5890.1749217455348923No Hit
GTATGAATGGCTCCACGAGGGTTCAGCTGTCTCTTACTTTTAA5480.1627455289526672No Hit
ACTATACTCAATTGATCCAATAACTTGACCAACGGAACAAGTT5290.15710289199992872No Hit
TGCTAAGACTTCACCAGTCAAAGCGAACTACTATACTCAATTG5280.15680591110767933No Hit
GTATTAGAGGCACCGCCTGCCCAGTGACACATGTTTAACGGCC5240.15561798753868178No Hit
GATAGAAACCGACCTGGATTACTCCGGTCTGAACTCAGATCAC5030.1493813888014445No Hit
CCATAGGGTCTTCTCGTCTTGCTGTGTCATGCCCGCCTCTTCA4720.14017498114171334No Hit
GTTAGGGTACCGCGGCCGTTAAACATGTGTCACTGGGCAGGCG4570.13572026775797247No Hit
CCCCTATACCTTCTGCATAATGAATTAACTAGAAATAACTTTG4510.13393838240447611No Hit
TTATTACCCTCACTGTCAACCCAACACAGGCATGCTCATAAGG4210.12502895563699432No Hit
GTTAGGTACTGTTTGCATTAATAAATTAAAGCTCCATAGGGTC4140.12295008939124856No Hit
GGATTACTCCGGTCTGAACTCAGATCACGTAGGACTTTAATCG3980.11819839511525829No Hit
CCTTTAATAGCGGCTGCACCATCGGGATGTCCTGATCCAACAT3870.11493160530051495No Hit
CTACTATACTCAATTGATCCAATAACTTGACCAACGGAACAAG3810.1131497199470186No Hit
CCCTATAGAAGAACTAATGTTAGTATAAGTAACATGAAAACAT3800.11285273905476921No Hit
ATCTATCACCCTATAGAAGAACTAATGTTAGTATAAGTAACAT3670.1089919874555271No Hit
GTACCCTAACCGTGCAAAGGTAGCATAATCACTTGTTCCTTAA3670.1089919874555271No Hit
ACCCTATGGAGCTTTAATTTATTAATGCAAACAGTACCTAACA3410.1012704842570429No Hit
ATATAGTACCGCAAGGGAAAGATGAAAAATTATAGCCAAGCAT3370.10008256068804533No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGGCGTA250.00549089429.612
GTACAAT400.001928367223.1250021
AGTATTA753.7245263E-722.215
GGTATCA4850.021.3608251
CACATGT1750.021.14285728
AACGGCC1500.020.96666737
TTAACGG1500.020.96666735
GCACCGC1700.020.67647210
GTGACAC1700.020.67647224
TCTACTC450.003820276620.5555553
AGTGACA1750.020.08571423
CACCGCC1850.020.011
TAACGGC1600.019.6562536
AGGCACC1800.019.5277778
CCTCTAG1153.0267984E-919.3043481
GGCACCG1850.019.09
ACCGCCT1950.018.9743612
GTATTAG2150.018.9302351
AAATCTA500.007024807418.518
ATTAGAG2000.018.53
Produced by FastQC (version 0.11.5)