FastQCFastQC Report
Fri 10 Feb 2017
ERR1633271.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1633271.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences396079
Sequences flagged as poor quality0
Sequence length43
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT14090.3557371130506793No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT10300.2600491316126328No Hit
GTACATGCTAAGACTTCACCAGTCAAAGCGAACTACTATACTC7270.18354924143920784No Hit
GTATTAGAGGCACCGCCTGCCCAGTGACACATGTTTAACGGCC7180.18127696747366057No Hit
GTATGAATGGCTCCACGAGGGTTCAGCTGTCTCTTACTTTTAA6340.16006907712855264No Hit
AGCATGTACTGCTCGGAGGTTGGGTTCTGCTCCGAGGTCGCCC6140.15501957942733646No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT6100.15400967988709321No Hit
TTATTACCCTCACTGTCAACCCAACACAGGCATGCTCATAAGG5710.14416315936972168No Hit
CCCCTATACCTTCTGCATAATGAATTAACTAGAAATAACTTTG5670.14315325982947846No Hit
GATAGAAACCGACCTGGATTACTCCGGTCTGAACTCAGATCAC5270.13305426442704613No Hit
CTCTAATACTGGTGATGCTAGAGGTGATGTTTTTGGTAAACAG5180.13078199046149883No Hit
CCATAGGGTCTTCTCGTCTTGCTGTGTCATGCCCGCCTCTTCA5000.1262374425304043No Hit
TGCTAAGACTTCACCAGTCAAAGCGAACTACTATACTCAATTG4940.12472259322003944No Hit
ACTATACTCAATTGATCCAATAACTTGACCAACGGAACAAGTT4910.12396516856485701No Hit
GTTAGGGTACCGCGGCCGTTAAACATGTGTCACTGGGCAGGCG4830.12194536948437054No Hit
CCCTATAGAAGAACTAATGTTAGTATAAGTAACATGAAAACAT4720.11916814574870166No Hit
CCTTTAATAGCGGCTGCACCATCGGGATGTCCTGATCCAACAT4610.11639092201303275No Hit
ATCTATCACCCTATAGAAGAACTAATGTTAGTATAAGTAACAT4470.11285627362218142No Hit
GTACCCTAACCGTGCAAAGGTAGCATAATCACTTGTTCCTTAA4430.1118463740819382No Hit
GCATAATATAGCAAGGACTAACCCCTATACCTTCTGCATAATG4400.11108894942675578No Hit
ATATAGTACCGCAAGGGAAAGATGAAAAATTATAGCCAAGCAT4360.11007904988651254No Hit
GGATTACTCCGGTCTGAACTCAGATCACGTAGGACTTTAATCG4250.10730182615084365No Hit
GTTAGGTACTGTTTGCATTAATAAATTAAAGCTCCATAGGGTC4200.1060394517255396No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CTACGCT200.001840479937.028
ACGCTGC200.001840479937.030
TGCGGTA250.005492049329.59999836
ATCTCCG358.859091E-426.42857210
GGTATCA3200.024.281251
AATCGTG400.00192896523.1257
AACCACG502.6977717E-422.19999935
GTGCTAG502.6977717E-422.19999927
CCACGTT603.7183523E-521.58333437
CGCCTAT450.00382144720.55555736
GCTAGTA555.134917E-420.18181829
ACGTCGG555.134917E-420.18181827
GTATCAA7550.019.8476831
CCCTAGA801.6123147E-518.51
TAACCAC801.6123147E-518.534
CTAGTAA609.2222483E-418.530
AACTCTC609.2222483E-418.519
ATGGGCG500.00702694318.49999821
TTGTCCG500.00702694318.49999813
CATCTCG500.00702694318.49999836
Produced by FastQC (version 0.11.5)