##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	ERR1633271.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	396079
Sequences flagged as poor quality	0
Sequence length	43
%GC	44
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	30.99577357042408	33.0	33.0	33.0	27.0	33.0
2	31.311326780768482	33.0	33.0	33.0	27.0	33.0
3	31.068238911934234	33.0	33.0	33.0	27.0	33.0
4	31.395577649913275	33.0	33.0	33.0	27.0	33.0
5	31.500996013421563	33.0	33.0	33.0	27.0	33.0
6	34.15874863347968	37.0	33.0	37.0	27.0	37.0
7	34.32036790640251	37.0	33.0	37.0	27.0	37.0
8	34.4660610635757	37.0	37.0	37.0	27.0	37.0
9	34.57974040532318	37.0	37.0	37.0	27.0	37.0
10	34.577293923686945	37.0	37.0	37.0	27.0	37.0
11	34.59632548052282	37.0	37.0	37.0	27.0	37.0
12	34.64100343618318	37.0	37.0	37.0	27.0	37.0
13	34.630573698681324	37.0	37.0	37.0	27.0	37.0
14	34.63234607237445	37.0	37.0	37.0	27.0	37.0
15	34.61897752721048	37.0	37.0	37.0	27.0	37.0
16	34.601635027355655	37.0	37.0	37.0	27.0	37.0
17	34.6257034581485	37.0	37.0	37.0	27.0	37.0
18	34.610756944952904	37.0	37.0	37.0	27.0	37.0
19	34.59316197021301	37.0	37.0	37.0	27.0	37.0
20	34.57935159400019	37.0	37.0	37.0	27.0	37.0
21	34.58304025207092	37.0	37.0	37.0	27.0	37.0
22	34.47862926335403	37.0	37.0	37.0	27.0	37.0
23	34.5329063141444	37.0	37.0	37.0	27.0	37.0
24	34.52945245771677	37.0	37.0	37.0	27.0	37.0
25	34.52658939252018	37.0	37.0	37.0	27.0	37.0
26	34.394024929370154	37.0	37.0	37.0	27.0	37.0
27	34.4097869364445	37.0	37.0	37.0	27.0	37.0
28	34.42855086990222	37.0	37.0	37.0	27.0	37.0
29	34.42301157092398	37.0	37.0	37.0	27.0	37.0
30	34.41702286665034	37.0	37.0	37.0	27.0	37.0
31	34.38615276245395	37.0	37.0	37.0	27.0	37.0
32	34.38244643114126	37.0	37.0	37.0	27.0	37.0
33	34.37357193893138	37.0	37.0	37.0	27.0	37.0
34	34.35445201588572	37.0	37.0	37.0	27.0	37.0
35	34.289565970425095	37.0	37.0	37.0	27.0	37.0
36	34.32473319716521	37.0	37.0	37.0	27.0	37.0
37	34.336498526809045	37.0	37.0	37.0	27.0	37.0
38	34.327810866014104	37.0	37.0	37.0	27.0	37.0
39	34.2289820970059	37.0	37.0	37.0	27.0	37.0
40	33.99449604750568	37.0	37.0	37.0	27.0	37.0
41	34.18936626279101	37.0	37.0	37.0	27.0	37.0
42	34.22557621080643	37.0	37.0	37.0	27.0	37.0
43	33.80542265558134	37.0	33.0	37.0	27.0	37.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
15	29.0
16	25.0
17	33.0
18	32.0
19	56.0
20	137.0
21	310.0
22	742.0
23	1486.0
24	2485.0
25	3907.0
26	5808.0
27	7789.0
28	10290.0
29	12647.0
30	15979.0
31	19294.0
32	24177.0
33	30973.0
34	43364.0
35	76856.0
36	139660.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	39.822863620641336	22.174869154890818	13.527099391788003	24.475167832679844
2	16.385620040446476	22.271567035869108	38.05553942521568	23.28727349846874
3	17.62653410052035	28.306979163247735	29.97003123114328	24.09645550508863
4	12.664645184420278	19.205512031690645	39.77842804087064	28.351414743018438
5	13.603094332191304	37.98434150762853	36.04255716662585	12.370006993554316
6	28.84879026658823	41.01732230186402	17.45510365356406	12.678783777983686
7	26.16624461281714	32.63919571600616	24.313836381125988	16.88072329005072
8	23.860643962441834	36.83053128290064	20.609272392628743	18.699552362028786
9	25.292681510506743	15.86501682745109	21.025351003208957	37.816950658833214
10	14.962671588243758	28.48547890698573	34.374455601029084	22.177393903741425
11	33.36783823429164	24.153767303997434	24.496880672795072	17.981513788915848
12	22.64043284294295	26.597471716501005	30.096773623443806	20.665321817112243
13	26.655540940064988	22.92168986490069	26.613377634259834	23.809391560774493
14	21.418454399248635	21.264949669131664	28.15448433267101	29.16211159894869
15	23.506421698701523	29.2322996169956	25.14296390366568	22.118314780637196
16	22.670982304035306	29.057082046763398	25.44896346435938	22.822972184841912
17	21.861042872760233	28.414028514513518	26.805258546906046	22.919670065820203
18	21.92618139310592	28.326672204282477	28.435741354628746	21.311405047982852
19	24.13129703922702	26.810055569722202	27.930034159851946	21.128613231198827
20	23.056763928408223	27.179678801451225	28.399132496294932	21.364424773845624
21	22.31246796724896	27.953261849277546	28.18326646956794	21.55100371390556
22	22.095844515866784	27.58717326593937	27.92927673519677	22.387705482997077
23	22.109478159660068	27.418267567833688	28.64302323526367	21.829231037242568
24	22.471527144837268	28.48143930882475	26.457600630177314	22.589432916160664
25	22.416740094779072	27.622267274962827	28.08050919134819	21.880483438909913
26	22.382403510410803	28.072429995026244	28.22063275255694	21.324533742006015
27	22.327868935237667	27.732093849964272	27.029203769954986	22.910833444843075
28	21.606043238848816	27.375851787143475	29.11590869498257	21.902196279025144
29	24.07095554169749	27.146352116623202	27.287485577372188	21.49520676430712
30	22.97900166380949	26.798189250124345	28.16029125502741	22.062517831038758
31	22.377858962479706	27.3518666730627	28.193617939855432	22.076656424602163
32	21.40456828057029	27.144332317542712	28.457201719858915	22.99389768202808
33	21.36669704781117	27.448312079155922	28.836166522335187	22.34882435069771
34	22.723749555013015	26.980223642253186	28.617523271872532	21.678503530861267
35	22.546512185700326	27.956039073013212	27.67730679990608	21.82014194138038
36	23.592263159622195	27.556623804847014	27.057733431966856	21.793379603563938
37	22.261972990236796	27.00850082938	27.721489904791717	23.008036275591486
38	22.704813938633457	27.47381204254707	27.515722873467162	22.305651145352314
39	21.84109735684043	27.65887613329664	27.99113308203666	22.50889342782627
40	22.21375028719018	28.153726908015823	28.19891991244171	21.433602892352283
41	21.952186306267187	26.963307824954114	28.20548425945329	22.879021609325413
42	21.62346400591801	28.175692223016114	27.89796984944923	22.302873921616648
43	22.755056440760555	26.45053133339561	27.98886080807112	22.80555141777272
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	19.0
1	18.0
2	17.0
3	79.5
4	142.0
5	142.0
6	171.0
7	200.0
8	195.5
9	191.0
10	271.5
11	352.0
12	352.0
13	546.5
14	741.0
15	1410.0
16	2079.0
17	2322.0
18	2565.0
19	2565.0
20	2570.0
21	2575.0
22	2911.0
23	3247.0
24	4337.0
25	5427.0
26	5427.0
27	7101.5
28	8776.0
29	10407.5
30	12039.0
31	14539.0
32	17039.0
33	17039.0
34	20956.0
35	24873.0
36	26922.0
37	28971.0
38	30995.0
39	33019.0
40	33019.0
41	34049.5
42	35080.0
43	35339.0
44	35598.0
45	33725.0
46	31852.0
47	31852.0
48	29634.0
49	27416.0
50	27036.0
51	26656.0
52	24800.5
53	22945.0
54	22945.0
55	22364.5
56	21784.0
57	18123.5
58	14463.0
59	13163.0
60	11863.0
61	11863.0
62	10582.5
63	9302.0
64	7460.5
65	5619.0
66	4589.0
67	3559.0
68	3559.0
69	2981.5
70	2404.0
71	2047.0
72	1690.0
73	1625.0
74	1560.0
75	1560.0
76	1305.0
77	1050.0
78	773.5
79	497.0
80	336.5
81	176.0
82	176.0
83	155.5
84	135.0
85	109.0
86	83.0
87	67.5
88	52.0
89	52.0
90	36.0
91	20.0
92	11.0
93	2.0
94	1.5
95	1.0
96	1.0
97	0.5
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
43	396079.0
>>END_MODULE
>>Sequence Duplication Levels	warn
#Total Deduplicated Percentage	57.6778229705015
#Duplication Level	Percentage of deduplicated	Percentage of total
1	89.0270467708329	51.34886243234658
2	5.762027480626067	6.646824019574302
3	1.528589817565507	2.644971986761635
4	0.8076856637287028	1.863422029134245
5	0.48885926961101034	1.4098169205056261
6	0.35608655731510847	1.2322978448997701
7	0.2640679706526888	1.0661605964439758
8	0.1943131121355644	0.8966045826081834
9	0.1836333683546783	0.9532415620294227
>10	1.1372844377041633	13.318325857048125
>50	0.1507770549725536	6.1347467993153995
>100	0.09473602569069417	10.451695830796124
>500	0.004002930662987078	1.4073438546892036
>1k	8.895401473304617E-4	0.625685683847424
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT	1409	0.3557371130506793	No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT	1030	0.2600491316126328	No Hit
GTACATGCTAAGACTTCACCAGTCAAAGCGAACTACTATACTC	727	0.18354924143920784	No Hit
GTATTAGAGGCACCGCCTGCCCAGTGACACATGTTTAACGGCC	718	0.18127696747366057	No Hit
GTATGAATGGCTCCACGAGGGTTCAGCTGTCTCTTACTTTTAA	634	0.16006907712855264	No Hit
AGCATGTACTGCTCGGAGGTTGGGTTCTGCTCCGAGGTCGCCC	614	0.15501957942733646	No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT	610	0.15400967988709321	No Hit
TTATTACCCTCACTGTCAACCCAACACAGGCATGCTCATAAGG	571	0.14416315936972168	No Hit
CCCCTATACCTTCTGCATAATGAATTAACTAGAAATAACTTTG	567	0.14315325982947846	No Hit
GATAGAAACCGACCTGGATTACTCCGGTCTGAACTCAGATCAC	527	0.13305426442704613	No Hit
CTCTAATACTGGTGATGCTAGAGGTGATGTTTTTGGTAAACAG	518	0.13078199046149883	No Hit
CCATAGGGTCTTCTCGTCTTGCTGTGTCATGCCCGCCTCTTCA	500	0.1262374425304043	No Hit
TGCTAAGACTTCACCAGTCAAAGCGAACTACTATACTCAATTG	494	0.12472259322003944	No Hit
ACTATACTCAATTGATCCAATAACTTGACCAACGGAACAAGTT	491	0.12396516856485701	No Hit
GTTAGGGTACCGCGGCCGTTAAACATGTGTCACTGGGCAGGCG	483	0.12194536948437054	No Hit
CCCTATAGAAGAACTAATGTTAGTATAAGTAACATGAAAACAT	472	0.11916814574870166	No Hit
CCTTTAATAGCGGCTGCACCATCGGGATGTCCTGATCCAACAT	461	0.11639092201303275	No Hit
ATCTATCACCCTATAGAAGAACTAATGTTAGTATAAGTAACAT	447	0.11285627362218142	No Hit
GTACCCTAACCGTGCAAAGGTAGCATAATCACTTGTTCCTTAA	443	0.1118463740819382	No Hit
GCATAATATAGCAAGGACTAACCCCTATACCTTCTGCATAATG	440	0.11108894942675578	No Hit
ATATAGTACCGCAAGGGAAAGATGAAAAATTATAGCCAAGCAT	436	0.11007904988651254	No Hit
GGATTACTCCGGTCTGAACTCAGATCACGTAGGACTTTAATCG	425	0.10730182615084365	No Hit
GTTAGGTACTGTTTGCATTAATAAATTAAAGCTCCATAGGGTC	420	0.1060394517255396	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	5.049497701216172E-4	0.0	0.0
2	0.0	0.0	5.049497701216172E-4	0.0	0.0
3	0.0	0.0	5.049497701216172E-4	0.0	0.0
4	0.0	0.0	5.049497701216172E-4	0.0	0.0
5	0.0	0.0	5.049497701216172E-4	0.0	0.0
6	0.0	0.0	0.0010098995402432343	0.0	0.0
7	0.0	0.0	0.0015148493103648514	2.524748850608086E-4	0.0
8	0.0	0.0	0.00176732419542566	2.524748850608086E-4	0.0
9	0.0	0.0	0.002272273965547277	7.574246551824257E-4	0.0
10	7.574246551824257E-4	0.0	0.002272273965547277	7.574246551824257E-4	0.0
11	7.574246551824257E-4	0.0	0.004039598160972937	7.574246551824257E-4	0.0
12	7.574246551824257E-4	0.0	0.004292073046033746	0.0010098995402432343	0.0
13	7.574246551824257E-4	0.0	0.004292073046033746	0.0010098995402432343	0.0
14	7.574246551824257E-4	0.0	0.004797022816155363	0.0010098995402432343	0.0
15	7.574246551824257E-4	0.0	0.005049497701216172	0.0010098995402432343	0.0
16	7.574246551824257E-4	0.0	0.00530197258627698	0.0010098995402432343	0.0
17	7.574246551824257E-4	0.0	0.005554447471337789	0.0010098995402432343	0.0
18	7.574246551824257E-4	0.0	0.005806922356398597	0.0010098995402432343	0.0
19	7.574246551824257E-4	0.0	0.006059397241459406	0.0010098995402432343	0.0
20	7.574246551824257E-4	0.0	0.006059397241459406	0.001262374425304043	0.0
21	7.574246551824257E-4	0.0	0.006311872126520214	0.001262374425304043	0.0
22	7.574246551824257E-4	0.0	0.006311872126520214	0.002272273965547277	0.0
23	7.574246551824257E-4	0.0	0.006311872126520214	0.003029698620729703	0.0
24	7.574246551824257E-4	0.0	0.006311872126520214	0.004039598160972937	0.0
25	7.574246551824257E-4	0.0	0.006311872126520214	0.004544547931094554	0.0
26	7.574246551824257E-4	0.0	0.006311872126520214	0.00530197258627698	0.0
27	7.574246551824257E-4	0.0	0.006311872126520214	0.006059397241459406	0.0
28	7.574246551824257E-4	0.0	0.006311872126520214	0.011613844712797194	0.0
29	7.574246551824257E-4	0.0	0.006311872126520214	0.02449006385089843	0.0
30	7.574246551824257E-4	0.0	0.006311872126520214	0.04064845649479018	0.0
31	0.0010098995402432343	0.0	0.006311872126520214	0.08786126000116139	0.0
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
CTACGCT	20	0.0018404799	37.0	28
ACGCTGC	20	0.0018404799	37.0	30
TGCGGTA	25	0.0054920493	29.599998	36
ATCTCCG	35	8.859091E-4	26.428572	10
GGTATCA	320	0.0	24.28125	1
AATCGTG	40	0.001928965	23.125	7
AACCACG	50	2.6977717E-4	22.199999	35
GTGCTAG	50	2.6977717E-4	22.199999	27
CCACGTT	60	3.7183523E-5	21.583334	37
CGCCTAT	45	0.003821447	20.555557	36
GCTAGTA	55	5.134917E-4	20.181818	29
ACGTCGG	55	5.134917E-4	20.181818	27
GTATCAA	755	0.0	19.847683	1
CCCTAGA	80	1.6123147E-5	18.5	1
TAACCAC	80	1.6123147E-5	18.5	34
CTAGTAA	60	9.2222483E-4	18.5	30
AACTCTC	60	9.2222483E-4	18.5	19
ATGGGCG	50	0.007026943	18.499998	21
TTGTCCG	50	0.007026943	18.499998	13
CATCTCG	50	0.007026943	18.499998	36
>>END_MODULE