FastQCFastQC Report
Fri 10 Feb 2017
ERR1633270.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1633270.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences683970
Sequences flagged as poor quality0
Sequence length43
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT26540.3880287147097095No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT18200.2660935421144202No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT11450.16740500314341272No Hit
GTATTAGAGGCACCGCCTGCCCAGTGACACATGTTTAACGGCC8550.1250054826966095No Hit
GTACATGCTAAGACTTCACCAGTCAAAGCGAACTACTATACTC7750.11330906326300862No Hit
TTATTACCCTCACTGTCAACCCAACACAGGCATGCTCATAAGG7670.11213942131964852No Hit
CCCCTATACCTTCTGCATAATGAATTAACTAGAAATAACTTTG7150.10453674868780795No Hit
AGCATGTACTGCTCGGAGGTTGGGTTCTGCTCCGAGGTCGCCC7070.10336710674444785No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA4950.026.535351
CGTCCGC551.9011986E-523.54545429
GACGGAT400.001930391623.1250027
TTAACGG2000.023.12500235
CGAGTCG502.7005523E-422.221
ACTACCG502.7005523E-422.25
TAACGGC2100.022.0238136
GTATCAA12850.021.5953311
AACGGCC2150.021.5116337
GAGTCGC555.140168E-420.18181822
CGCCGTC555.140168E-420.18181826
AACCCCG609.231604E-418.530
TTGTCCG701.2182817E-418.513
TGCGGGT609.231604E-418.521
TGACCGG500.007032040518.510
ATCTTAC1205.162292E-918.51
GTGACAC2550.018.13725524
TTTAACG2650.017.45283134
TATACTG1401.8644641E-917.1785725
TCGCCGT650.0015790517.07692325
Produced by FastQC (version 0.11.5)