##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	ERR1633270.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	683970
Sequences flagged as poor quality	0
Sequence length	43
%GC	45
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.469507434536602	33.0	33.0	33.0	27.0	33.0
2	31.66496483763908	33.0	33.0	33.0	27.0	33.0
3	31.397925347602964	33.0	33.0	33.0	27.0	33.0
4	31.71469216486103	33.0	33.0	33.0	27.0	33.0
5	31.813123382604502	33.0	33.0	33.0	33.0	33.0
6	34.84751231779172	37.0	37.0	37.0	27.0	37.0
7	35.0113309063263	37.0	37.0	37.0	33.0	37.0
8	35.15977016535813	37.0	37.0	37.0	33.0	37.0
9	35.289903065923944	37.0	37.0	37.0	33.0	37.0
10	35.28994400339196	37.0	37.0	37.0	33.0	37.0
11	35.2988581370528	37.0	37.0	37.0	33.0	37.0
12	35.297124142871766	37.0	37.0	37.0	33.0	37.0
13	35.30470049855988	37.0	37.0	37.0	33.0	37.0
14	35.28805064549615	37.0	37.0	37.0	33.0	37.0
15	35.275327865257246	37.0	37.0	37.0	33.0	37.0
16	35.26584060704417	37.0	37.0	37.0	33.0	37.0
17	35.27088468792491	37.0	37.0	37.0	33.0	37.0
18	35.253419009605686	37.0	37.0	37.0	33.0	37.0
19	35.240215214117576	37.0	37.0	37.0	33.0	37.0
20	35.2395002704797	37.0	37.0	37.0	33.0	37.0
21	35.229897510124715	37.0	37.0	37.0	33.0	37.0
22	35.1397605158121	37.0	37.0	37.0	33.0	37.0
23	35.18223460093279	37.0	37.0	37.0	33.0	37.0
24	35.17403687296226	37.0	37.0	37.0	33.0	37.0
25	35.191362194248285	37.0	37.0	37.0	33.0	37.0
26	35.075184584119185	37.0	37.0	37.0	33.0	37.0
27	35.08099039431554	37.0	37.0	37.0	33.0	37.0
28	35.101505914002075	37.0	37.0	37.0	33.0	37.0
29	35.10556603359797	37.0	37.0	37.0	33.0	37.0
30	35.10295042180213	37.0	37.0	37.0	33.0	37.0
31	35.0939763439917	37.0	37.0	37.0	33.0	37.0
32	35.084850212728625	37.0	37.0	37.0	33.0	37.0
33	35.07292571311607	37.0	37.0	37.0	33.0	37.0
34	35.06095735193064	37.0	37.0	37.0	27.0	37.0
35	34.99445297308362	37.0	37.0	37.0	27.0	37.0
36	35.01339678640876	37.0	37.0	37.0	27.0	37.0
37	35.0224615114698	37.0	37.0	37.0	27.0	37.0
38	35.00567568753016	37.0	37.0	37.0	27.0	37.0
39	34.903940231296694	37.0	37.0	37.0	27.0	37.0
40	34.68335745719842	37.0	37.0	37.0	27.0	37.0
41	34.86413877801658	37.0	37.0	37.0	27.0	37.0
42	34.90234074593915	37.0	37.0	37.0	27.0	37.0
43	34.53971957834408	37.0	37.0	37.0	27.0	37.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
14	4.0
15	19.0
16	42.0
17	20.0
18	27.0
19	39.0
20	95.0
21	224.0
22	565.0
23	1247.0
24	2421.0
25	4168.0
26	6473.0
27	9220.0
28	12488.0
29	16364.0
30	20867.0
31	25812.0
32	32717.0
33	42036.0
34	60282.0
35	115957.0
36	332883.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	40.66859657587321	21.132067195929647	13.639925727736596	24.559410500460547
2	16.18974516426159	21.795254177814815	38.3582613272512	23.656739330672398
3	17.821834290977677	27.258505490006872	30.00029241048584	24.919367808529614
4	12.51648464113923	18.128280480138017	39.54208517917452	29.813149699548227
5	13.80981622000965	37.443893738029445	35.53869321753878	13.207596824422124
6	29.443104229717676	40.22325540593886	17.285553459947074	13.048086904396392
7	26.404228255625245	32.32583300437153	23.748117607497406	17.52182113250581
8	24.418761056771494	36.176586692398786	20.470927087445357	18.93372516338436
9	25.728467622848957	15.38400807052941	20.53233328947176	38.35519101714988
10	14.774770823281722	28.26249689313859	34.1608550082606	22.801877275319093
11	33.84680614646841	23.726771642031082	24.284106028042167	18.14231618345834
12	22.6543561852128	26.002602453323973	30.440662602160913	20.90237875930231
13	27.53483339912569	22.574528122578474	26.346184774186003	23.54445370410983
14	21.61162039270728	21.115692208722606	27.927394476365926	29.34529292220419
15	23.762445721303564	28.97261575800108	24.551807827828707	22.713130692866645
16	22.741494509993128	28.70125882714154	25.420266970773568	23.136979692091757
17	22.165007237159525	27.975057385557843	26.70175592496747	23.15817945231516
18	22.65537962191324	27.680453821074025	27.970525023027328	21.693641533985407
19	24.43528224922146	26.670468002982588	27.587028670848134	21.30722107694782
20	23.727941283974445	26.71447578110151	27.76101290992295	21.796570025001095
21	22.66546778367472	27.274003245756397	27.823150138163953	22.23737883240493
22	22.453470181440707	27.155576998991183	27.62781993362282	22.76313288594529
23	22.48914426071319	26.97282044534117	28.16059183882334	22.3774434551223
24	22.76108601254441	27.63381434858254	26.673830723569747	22.931268915303303
25	22.80757927979297	27.158501103849588	27.846104361302398	22.187815255055046
26	22.831703144874776	27.417869204789685	27.741128996885827	22.00929865344971
27	22.695732268959166	27.288769975291316	27.182332558445545	22.833165197303977
28	22.197757211573606	27.105867216398384	28.08149480240361	22.6148807696244
29	23.625012792958756	26.90746670175593	27.178677427372545	22.288843077912773
30	23.247803266225127	26.791379738877435	27.60106437416846	22.35975262072898
31	22.981417313624867	26.937584981797446	27.547699460502656	22.53329824407503
32	21.856514174598303	26.81623463017384	28.040557334386012	23.28669386084185
33	22.012076553065192	27.02808602716493	28.27682500694475	22.683012412825125
34	22.681257949910083	26.797374153837154	28.05590888489261	22.46545901136015
35	22.647045923066802	27.49842829363861	27.485854642747487	22.3686711405471
36	23.494743921517024	27.11361609427314	27.200462008567627	22.191177975642205
37	22.65377136424112	26.554381040104097	27.584250771232654	23.207596824422126
38	22.843399564308374	27.21464391713087	27.401201807096804	22.540754711463954
39	22.341623170606898	27.058642922935217	27.77373276605699	22.826001140400894
40	22.563416524116555	27.625334444493177	27.826659063994036	21.984589967396232
41	22.591341725514276	26.612424521543343	27.77987338625963	23.01636036668275
42	22.21135429916517	27.49842829363861	27.75545711069199	22.534760296504235
43	22.93287717297542	26.486834217875057	27.64185563694314	22.938432972206382
>>END_MODULE
>>Per sequence GC content	pass
#GC Content	Count
0	23.0
1	33.5
2	44.0
3	149.0
4	254.0
5	254.0
6	324.5
7	395.0
8	390.0
9	385.0
10	523.5
11	662.0
12	662.0
13	1093.0
14	1524.0
15	2605.0
16	3686.0
17	4359.0
18	5032.0
19	5032.0
20	5044.0
21	5056.0
22	5568.5
23	6081.0
24	8028.5
25	9976.0
26	9976.0
27	12725.5
28	15475.0
29	18245.5
30	21016.0
31	24434.5
32	27853.0
33	27853.0
34	32855.5
35	37858.0
36	41217.0
37	44576.0
38	47656.0
39	50736.0
40	50736.0
41	52378.0
42	54020.0
43	55170.0
44	56320.0
45	54798.5
46	53277.0
47	53277.0
48	50965.5
49	48654.0
50	48341.5
51	48029.0
52	45526.0
53	43023.0
54	43023.0
55	41476.0
56	39929.0
57	34698.5
58	29468.0
59	27355.5
60	25243.0
61	25243.0
62	22351.5
63	19460.0
64	16121.0
65	12782.0
66	10732.5
67	8683.0
68	8683.0
69	7198.5
70	5714.0
71	4621.0
72	3528.0
73	2851.5
74	2175.0
75	2175.0
76	1675.5
77	1176.0
78	945.5
79	715.0
80	589.0
81	463.0
82	463.0
83	376.5
84	290.0
85	246.0
86	202.0
87	162.0
88	122.0
89	122.0
90	89.5
91	57.0
92	32.5
93	8.0
94	4.0
95	0.0
96	0.0
97	0.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
43	683970.0
>>END_MODULE
>>Sequence Duplication Levels	warn
#Total Deduplicated Percentage	61.43315155602915
#Duplication Level	Percentage of deduplicated	Percentage of total
1	86.37608812483144	53.063553125897
2	7.914076194267286	9.72373284536769
3	2.1709144438225567	4.000983481275715
4	0.9500120375811436	2.3344893393909785
5	0.5513385412158934	1.6935232080598002
6	0.3570463951225514	1.316069118245854
7	0.2479200170818866	1.066135558821541
8	0.1879465702666011	0.9236920108499189
9	0.14302386815608562	0.7907766271706106
>10	0.9287390091933632	11.075425043685486
>50	0.10171703733319647	4.400156782649466
>100	0.06757077998975472	7.640952690438343
>500	0.002885584910570205	1.1404407000599364
>1k	7.213962276425512E-4	0.830069468087666
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT	2654	0.3880287147097095	No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT	1820	0.2660935421144202	No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT	1145	0.16740500314341272	No Hit
GTATTAGAGGCACCGCCTGCCCAGTGACACATGTTTAACGGCC	855	0.1250054826966095	No Hit
GTACATGCTAAGACTTCACCAGTCAAAGCGAACTACTATACTC	775	0.11330906326300862	No Hit
TTATTACCCTCACTGTCAACCCAACACAGGCATGCTCATAAGG	767	0.11213942131964852	No Hit
CCCCTATACCTTCTGCATAATGAATTAACTAGAAATAACTTTG	715	0.10453674868780795	No Hit
AGCATGTACTGCTCGGAGGTTGGGTTCTGCTCCGAGGTCGCCC	707	0.10336710674444785	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	0.0	0.0
3	0.0	0.0	2.9241048584002224E-4	0.0	0.0
4	0.0	0.0	2.9241048584002224E-4	0.0	0.0
5	0.0	0.0	4.3861572876003333E-4	0.0	0.0
6	0.0	0.0	7.310262146000556E-4	0.0	0.0
7	0.0	0.0	0.0017544629150401333	1.4620524292001112E-4	0.0
8	0.0	0.0	0.0021930786438001666	2.9241048584002224E-4	0.0
9	0.0	0.0	0.0029241048584002223	8.772314575200667E-4	0.0
10	0.0	0.0	0.0035089258300802667	8.772314575200667E-4	0.0
11	0.0	0.0	0.0045323625305203445	8.772314575200667E-4	0.0
12	0.0	0.0	0.004678567773440356	8.772314575200667E-4	0.0
13	0.0	0.0	0.004970978259280378	8.772314575200667E-4	0.0
14	0.0	0.0	0.0052633887451204	8.772314575200667E-4	0.0
15	0.0	0.0	0.005848209716800445	8.772314575200667E-4	0.0
16	0.0	0.0	0.006140620202640466	8.772314575200667E-4	0.0
17	0.0	0.0	0.0065792359314005	8.772314575200667E-4	0.0
18	0.0	0.0	0.0067254411743205116	8.772314575200667E-4	0.0
19	0.0	0.0	0.007017851660160533	8.772314575200667E-4	0.0
20	0.0	0.0	0.007310262146000556	8.772314575200667E-4	0.0
21	1.4620524292001112E-4	0.0	0.007310262146000556	0.001169641943360089	0.0
22	1.4620524292001112E-4	0.0	0.007456467388920566	0.0016082576721201222	0.0
23	1.4620524292001112E-4	0.0	0.007602672631840578	0.0019006681579601444	0.0
24	1.4620524292001112E-4	0.0	0.007602672631840578	0.0026316943725602	0.0
25	1.4620524292001112E-4	0.0	0.007602672631840578	0.003070310101320233	0.0
26	1.4620524292001112E-4	0.0	0.007602672631840578	0.0032165153442402445	0.0
27	1.4620524292001112E-4	0.0	0.007602672631840578	0.0040937468017603115	0.0
28	1.4620524292001112E-4	0.0	0.007602672631840578	0.011111598461920844	0.0
29	1.4620524292001112E-4	0.0	0.007602672631840578	0.02090734973756159	0.0
30	2.9241048584002224E-4	0.0	0.007602672631840578	0.041376083746363146	0.0
31	2.9241048584002224E-4	0.0	0.007602672631840578	0.0881617614807667	0.0
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GGTATCA	495	0.0	26.53535	1
CGTCCGC	55	1.9011986E-5	23.545454	29
GACGGAT	40	0.0019303916	23.125002	7
TTAACGG	200	0.0	23.125002	35
CGAGTCG	50	2.7005523E-4	22.2	21
ACTACCG	50	2.7005523E-4	22.2	5
TAACGGC	210	0.0	22.02381	36
GTATCAA	1285	0.0	21.595331	1
AACGGCC	215	0.0	21.51163	37
GAGTCGC	55	5.140168E-4	20.181818	22
CGCCGTC	55	5.140168E-4	20.181818	26
AACCCCG	60	9.231604E-4	18.5	30
TTGTCCG	70	1.2182817E-4	18.5	13
TGCGGGT	60	9.231604E-4	18.5	21
TGACCGG	50	0.0070320405	18.5	10
ATCTTAC	120	5.162292E-9	18.5	1
GTGACAC	255	0.0	18.137255	24
TTTAACG	265	0.0	17.452831	34
TATACTG	140	1.8644641E-9	17.178572	5
TCGCCGT	65	0.00157905	17.076923	25
>>END_MODULE